LOCUS BBa_K1377001 7772 bp DNA linear 26-Dec-2024 DEFINITION spaA backward + sortase A + spaB + spaC ACCESSION BBa_K1377001 VERSION BBa_K1377001.1 FEATURES Location/Qualifiers ORIGIN 1 agtcgtcgag tttgaggcac ctgtgaccaa gcagccagaa aacggccagc tagataacca 61 agcatgggta ctaccgagta accccactgc tcagtgggat ccagaagaaa gcggcgatgc 121 tgcgttgcgc ggtatgccgt cgtcacgcgt gagttccaag ttcggccaga tcacgattga 181 gaaatccttt gacggcaaca ccccaggtgc cgatcgcacc gcgacgttcc agctgcatcg 241 ttgcgaggcc gacggcagcc tagtcaagtc cgacccaccg atctcccttg atggcaagca 301 ggagtttgtt accggccaag atggtaaggc cgtactcagc ggtatccact tgggcacctt 361 gcagctcgag tccaatgtta tgaaatatac cgacgcgtgg gccggcaagg gaaccgaatt 421 ctgcctggta gaaacagcta ctgccagcgg atatgagctg ttgcctaagc cagtgatagt 481 aaagctggaa gccaacgagt ctaccaacgt gttggtagaa caaaaagtaa agatcgacaa 541 caagaaaaag aacgccggat ttgaactgcc actcacaggt ggttcggggc gcatcgccat 601 cactatcggc atcgttggtt tgctcgtcgc cctcgcaagc tatgtcttat cgcgtaggaa 661 ggacaaccgc tagatgaggc accgggcagg tgaacatcga aacgtttttg ccatcctcgc 721 attcgtcatc gcgatcgtct cagtgggatt tttgctctac cccgtggccg caaccgcatg 781 gaacaacgcg cgccaagcac gcgtcgcaca gtcctacgag aacagctacg aggtagacag 841 cccagcggta cgagacagcg ttcttgaggc ggccagacag tacaacacgt cggtagtagg 901 cttcccgatc ctcgatccgt ggctgaacag ggcgtcgaaa aacagcgggc catacctcga 961 ctacctgcaa caactcaacc cgcagcgcgc cgaacgtccc gtcatagcgt cgataagcat 1021 ccctactatc gacgcccacc tgcccatcta ccacggcacc gacaccgcca ccctcgagca 1081 cggactcggc cacctatacg gctccgcgct acccgttggc ggcaccggca cccaccccgt 1141 gatcaccgga cacagcggcc ttgccaacgc caccctcttt gacaacctcg aagacgtcaa 1201 agaacacgac cccatctaca tcaccgtcca aggcgaaacc ctcaaatacg aagtagacgc 1261 catcaacgta gtcctacccg aagacaccaa actcctcgcc ccagacccca acaaagacca 1321 aataacactc atcacctgca ccccctacgc cgtcaactcc caccgactcc tcgtacgagc 1381 ccaccgcgta gacctcgacc ccaacgaccc caacctcaca caaaccggca ccaaaatctg 1441 gcaaccctgg atgctgtgga ccgcagccct agcactcacc gccatcgcca tcatcatcac 1501 cctcgtgctt cgcaggaaga ggacaaccac ccatgaaaaa taacgccatc aacgccacag 1561 tgctcagcct cgcactgctc accggaacca cacccgcaca cgcacaagaa gcaaacacat 1621 tggtcattga cctcgaacca cctgcagcat tcgccgacga ccaaccccaa ggccacaaca 1681 tcgacgtcac cgtagcaaaa cttcacaaca tcgaccccga agaccacgaa cgcatccgcg 1741 cccttcaacg caacggcgta cccagcacca tcagcgaaga acaaccccac accgcgcgta 1801 ccgacgccac cggcaccgcc accatcacca acctgccacc agggacctac gtaatccgcg 1861 acaccaacac cacgaagccg cgctttagcc cccttgtgat cccactcggt atcgacacaa 1921 ccagccccac catgaccctg cgccccaagc ttatcgacgc cacccccggt gcaccgaacg 1981 tgcctagcgt acctagtccg ccgagcgtga cttctccggc tccgaaaaag acaccaccgc 2041 gactggcctt caccggcgca agcatcctcg gactgctggc catcgccacc atcagcaccc 2101 tcatcggaat cgcactacta cgcactcgac gtgcagaaaa gaaaggataa gacgtgagta 2161 acctacgcac catcaagaaa cgagcgtcta tccccgcagc actcgtcgcc atcctcgcaa 2221 tggtcatgag cattgttctt gtgccgttaa ttgcagcgcc atcagcgaat gcggagccac 2281 tgccgaaaaa agagtttgaa acctgtggcg gttctgttgc gatttccttt gacttgtcca 2341 attccctgag cgcttcggac gtggaaaaat ctaagcaagc agcgttggag ctggtcaaga 2401 gcttgaaagg atctccctat cgttttggta tttatacctt tgcttcacac tcgcctgctg 2461 ctggaaacaa aaatttcacg ccagtaagtc ttgctaatga tgacggatac aacaaagttg 2521 ttgccgctat caatgacatc cagatgccag cgatccgaga gaacaaaaag ggttctccca 2581 acggtggtac caactgggag ggcgggctcc aagcaatcgc gaatgacata gacagaggca 2641 tcaagtatga cgccgtttac ttcatcactg atggtcaacc aacttgggat aacaatggga 2701 gaaattggtt gggaaccacc accgaggttg tggaattaga aaatgccgtt acccaagcca 2761 aacttatttc tgataaaggc gcaaaactta ttccggtggg tattggccag ctttctgatg 2821 ataagccgtt tgatctctat aaaccgattc ttccttccga agacgattac tattggtcgc 2881 gttatccatg gaaaatagat cgttccctga ccggcaaaca aatgctggag aagataacct 2941 caccgggcct agagccaatt attttgcccg actattccac attgccgcag cgaatgggac 3001 aacagatttt taccggatgt ttccaaatcg ctaaaaacat tattgatgca gacggaaacg 3061 tgatagaaaa tccagctggc tggaatttcg atattacagc ggctggtgtg caaggcattc 3121 ctccgtcgat cgagacagat aaaaatggcc aagacacctt tgctatgaag tcgattaata 3181 aggaatcctt taagatcact atcactgagc gacctaccgg agatcaaaaa caaaacttcc 3241 ggtttaaaaa cgcacgctgc cagcgctact cctatgggca agcacctact gatattccga 3301 tcaaaactag cgatacttca attactttga ctgcagacac caaaagcttg atttcttgcg 3361 gttttaacaa tctgccagtt gtaccagtct cggtttcgaa aaaagtaaac gtaaatacgc 3421 cacaactttt agaagagctc aacaatcaaa cctttgattt cacctatagc tgtgaaaaag 3481 gagctaatga aaaagaaatc aagggagaaa ttaagggcgt ccacaacgga gaatccaaag 3541 aaatcggaaa agttgctgtt ggaactcagt gcgaaatcaa ggaagttacc cccaaagtcg 3601 acgattctcg gatgaagctt tccaccactt ggagcagtga aaacaccact gcagatgcta 3661 atcaggataa cggtacatac cgctttaaag ccgacaccga tgcgtttaaa aacaagaaaa 3721 cagttctagc tacagcagaa aataactatg aggctcaaac agccactatt aagctgacca 3781 agaccatcat taaccgtgac aaaattccag caacaaaact gcccgagaag tttcctgtca 3841 cttacacctg tcgttacgta ccacatccta atgctcgccc cgaacatggt gggctcccag 3901 aaaccaatcc gtattttgta gactctaaaa ccgttgtcgt tcctcgtgat ggaagtatag 3961 aaatcggacc ttttccagtg ggaacacagt gcagttttga agaaactgca cgactcgatc 4021 cgaatgttca agcagacgct aaaattcctg gttttagttt gaaaactgag tggaattcca 4081 acatctgttt tggaaacact atcgataata attctcaaga ttgttctact aactcagtat 4141 ggattcccaa accaggtcaa tattcgctca acgtagaaaa tacatacacg cgtgagcttg 4201 cgagcgtgga gatcgaaaag acggtgagcg gcgatgcctc tgatctcacg aattcacacg 4261 agttttcatt caatcttcga tgtgaagatt ccggagtaga agtctattcg caagacaata 4321 tcgtggtgaa gaaggacgga cggcaagtca tcgaaaacat tcctgtcgat gccaattgta 4381 cgttgagcga aaaacagcct gaacaaaaag gcgtggattt tgtggtcccc gcgccgttcc 4441 atcttcgtgc ttcaactgcc ggcgacattg tcaaagtggt tgtagataac accgcaaaac 4501 gtcaggtagc tcctatttca atacagaaaa aagttcataa aaaagacaca ttttctcctg 4561 aaatttctgc atcaatcgat gcattaacat acagtgtggt ggcagaatgt acggttcctg 4621 gtgtagaaac gcctcgaaaa gttctaaaaa cagtaagtga taatcaaact gttgaatttg 4681 gaaactttcc agtgggaact acttgtagct ttagcgagct caccgaagcc cctgccggaa 4741 ccgaaatgag ttataaattc gcggatggtc cagaggtgac aattgaggac tccactccta 4801 taaataaggt gctgacgaat acgtttgaaa atgcacgtgg cgagctaaaa gtaaccaaaa 4861 aagtactcga tggtgatatg cctcaagcat tagtagacca gattccatcg agttttacag 4921 tcaacgtcgc atgctcaatc accggtaatc attccatcac tttgcaaaaa gatgagcaga 4981 aagccgtacc tggggttgtt gcaggtgaaa gctgcacatt aagtgaggaa gtaactccta 5041 taactggggc tacccatcac aagcactgga ttaaaggcga gctgcttgaa gttgcagatt 5101 ctacggacat cacgattaac cctaatggta gtaacgcaat tcgattggaa aaccattacg 5161 aaaccgatgc tgtatctttg gaacttacca aacgtgttcg ggtcattgac caagttggaa 5221 atgacgttaa ctcggaacta aaaaatgcag ttgtccgtcc agaacaaccc ttcctattcc 5281 gataccgttg tgaaatcaat ggtcaagtag ttgcagaaaa taccttaagc gccgatgcga 5341 ttaacactgg tgccactaag gtgccacggg gatctatttg tacggttgaa gaagattcct 5401 cttcagtgga gttgtctaat gcaacgttat ctcacgttga gttcttcgtt cacggaacaa 5461 aaacgaatga taaggcatcg gtagcgataa actcggatca taaccgacta gatgctacta 5521 atactttcac gttgaagact ggctcattta accttaaaaa gaaagtcgat ggtgaaggag 5581 tatctaccat ccatgaggat cgacgctttg aaatttcgta tcgttgtacc ttaggcgact 5641 ggaagaaaaa cggcaccatt acgctgggac gttttgatag tgccgaatcg cattctgtta 5701 aagacattcc cgtgggtgca tcatgtgaga ttattgagga ctctgagaaa gcccaagagc 5761 caaacgcaca agtgacagct cgttggactc atacagacag cacgaatggc tggggcgata 5821 ccgaagcagc atgcgaaaat catgcagcgt gcgaagtgga tccaaaaaat gagtttgcaa 5881 ccacagtggt tattgctgga aatgagaaag agaatttcca aggaaccttt atcgtatgga 5941 acacctacac ttacgataaa acaaaggtag agatcaacaa ggtgttgacg aatgatggtc 6001 cagaacttgc tggtaaagat aactttgcct tcaccttgaa atgtactgat cctcgttttg 6061 caggaagtga tttggcagat aagcattcca ttccagaccc cacaattaca gttgcattaa 6121 atgctaaagg ccaaagccga gcgtcgtacc aagttgcaga cgaacggcac gatagcgttg 6181 aggttcctgt tgggtataac tgcactgtga ccgaaaaccc gattgcactt tatgatgcca 6241 aagcgacgac ccaattcagt ggtccggcag tggtggaaaa tacggctgtg caacgcacat 6301 catcaaactc cgcctcggct cgttttgtca cggagaaaca agaaaataat ggcactcaaa 6361 aaattcaggt aactaatgat tacattcgtc cgcgcgccga tgtcatggtg cataagacaa 6421 tcgcaaaacc agaacactcg gtagatcctt ggttgcttaa cactacatac agcatcactt 6481 ataagtgcga cgatccatac atcaaggatc gttcctattc aaacgacgta gatatacaag 6541 ctgatgcaga aaaaccaacg ccaattttcg ctgatcctac ggctcacgta aaaattcctg 6601 cgtcggcagt atgtactttc agtgaaaaca ccgaagggca tttaccagga gaggtaaaag 6661 gcgtagtgga tgaaacgaat aaagttgctg aattcgctgg ggaacatgaa aagcgctcct 6721 atttcacccc agaaattaaa gatgttgttt tgtcggaatc tgaaccaaca cgaattgaat 6781 tcaccaattc atacgtgatg cctcaacgaa ttttgagcct acaaaaatat gttgagggcg 6841 accccggcca tgctgtgatt gctccagaag aaacatttga attctcctac acctgcacca 6901 tgccgcatct attcccaaat caacccaatc ctatgtcgca agaagtagga aacaaggttg 6961 cacgtggcgt cattaagatt cgagaaggtg agacatggcg atctcctgaa gtccctattg 7021 gtacgtcctg cacgatcaag gaagaagacg accccgcctt gcgcaccaag ttggaaaaca 7081 atgcgctgcg catggtgcct acctacttgt tccccacgga gcgtgcagga gctgctagtg 7141 cgccagtgat tccgccgttg acagaccgtc cgatttataa cggcacggag cctcgcctcc 7201 agatgccaga atcaggcatt gagcttaacg acgcccactc gcacaccgtg gtgatcaaca 7261 acgtgtacac cactgacgct gagatcaaca ttgccaaggt gaacgccgat aactctccgc 7321 tgcccggcgc gcacttcgcc atctatggga taggggagaa tggccagcgt aaagagttgc 7381 ctgaggttgc ggatgcgccg gcgaagtcgg cgaagtcggt ggagcaggcg ttgtttgcag 7441 tgcgcttgcg ccctggtagt tacgagttgg tggagactca ggctcctcag ggtgggcagt 7501 tgctgcctaa gccgtggcgt tttgatgtca aggctgcgaa tgcgggtgcg ataggtgatc 7561 ttgaggtgac cttggataac tatgatgctg attcggggtt gatcacggtg gagcacccgc 7621 agggtaagcc gtggttgatc aaggtggcta atgtgtcggc atccacactg ccgttgactg 7681 gttcgaatgg ttacttgcgg tggctgttgg ccggtgctgc gggcctgttg gtggctgcag 7741 cattgtggtt agtggcgcgt cgtaagcgtt ag //