BBa_K1377001 1 BBa_K1377001 spaA backward + sortase A + spaB + spaC 2014-10-08T11:00:00Z 2015-05-08T01:10:12Z Genomic DNA of Corynebacterium diphtheriae Pilin A gene cluster is coding for pili A of Corynebacterium diphtheriae. It has four coding regions and one regulatory region. Regulatory region is its own promoter. Four coding regions are sortase A, enzyme that makes pili A and pilin spa A & B & C that are components of pili A . WIth these all regions, we can make pili A. And we are going to modifying this part to generate "Our Favorite Protein Whip", especially spa A false false _1754_ 0 15620 9 It's complicated false Just include all cluster that related to make pili A And there is no good restriction enzyme unique cut site, so we slice the cluster to two false Jade Minseob Yoo BBa_K1377001_sequence 1 agtcgtcgagtttgaggcacctgtgaccaagcagccagaaaacggccagctagataaccaagcatgggtactaccgagtaaccccactgctcagtgggatccagaagaaagcggcgatgctgcgttgcgcggtatgccgtcgtcacgcgtgagttccaagttcggccagatcacgattgagaaatcctttgacggcaacaccccaggtgccgatcgcaccgcgacgttccagctgcatcgttgcgaggccgacggcagcctagtcaagtccgacccaccgatctcccttgatggcaagcaggagtttgttaccggccaagatggtaaggccgtactcagcggtatccacttgggcaccttgcagctcgagtccaatgttatgaaatataccgacgcgtgggccggcaagggaaccgaattctgcctggtagaaacagctactgccagcggatatgagctgttgcctaagccagtgatagtaaagctggaagccaacgagtctaccaacgtgttggtagaacaaaaagtaaagatcgacaacaagaaaaagaacgccggatttgaactgccactcacaggtggttcggggcgcatcgccatcactatcggcatcgttggtttgctcgtcgccctcgcaagctatgtcttatcgcgtaggaaggacaaccgctagatgaggcaccgggcaggtgaacatcgaaacgtttttgccatcctcgcattcgtcatcgcgatcgtctcagtgggatttttgctctaccccgtggccgcaaccgcatggaacaacgcgcgccaagcacgcgtcgcacagtcctacgagaacagctacgaggtagacagcccagcggtacgagacagcgttcttgaggcggccagacagtacaacacgtcggtagtaggcttcccgatcctcgatccgtggctgaacagggcgtcgaaaaacagcgggccatacctcgactacctgcaacaactcaacccgcagcgcgccgaacgtcccgtcatagcgtcgataagcatccctactatcgacgcccacctgcccatctaccacggcaccgacaccgccaccctcgagcacggactcggccacctatacggctccgcgctacccgttggcggcaccggcacccaccccgtgatcaccggacacagcggccttgccaacgccaccctctttgacaacctcgaagacgtcaaagaacacgaccccatctacatcaccgtccaaggcgaaaccctcaaatacgaagtagacgccatcaacgtagtcctacccgaagacaccaaactcctcgccccagaccccaacaaagaccaaataacactcatcacctgcaccccctacgccgtcaactcccaccgactcctcgtacgagcccaccgcgtagacctcgaccccaacgaccccaacctcacacaaaccggcaccaaaatctggcaaccctggatgctgtggaccgcagccctagcactcaccgccatcgccatcatcatcaccctcgtgcttcgcaggaagaggacaaccacccatgaaaaataacgccatcaacgccacagtgctcagcctcgcactgctcaccggaaccacacccgcacacgcacaagaagcaaacacattggtcattgacctcgaaccacctgcagcattcgccgacgaccaaccccaaggccacaacatcgacgtcaccgtagcaaaacttcacaacatcgaccccgaagaccacgaacgcatccgcgcccttcaacgcaacggcgtacccagcaccatcagcgaagaacaaccccacaccgcgcgtaccgacgccaccggcaccgccaccatcaccaacctgccaccagggacctacgtaatccgcgacaccaacaccacgaagccgcgctttagcccccttgtgatcccactcggtatcgacacaaccagccccaccatgaccctgcgccccaagcttatcgacgccacccccggtgcaccgaacgtgcctagcgtacctagtccgccgagcgtgacttctccggctccgaaaaagacaccaccgcgactggccttcaccggcgcaagcatcctcggactgctggccatcgccaccatcagcaccctcatcggaatcgcactactacgcactcgacgtgcagaaaagaaaggataagacgtgagtaacctacgcaccatcaagaaacgagcgtctatccccgcagcactcgtcgccatcctcgcaatggtcatgagcattgttcttgtgccgttaattgcagcgccatcagcgaatgcggagccactgccgaaaaaagagtttgaaacctgtggcggttctgttgcgatttcctttgacttgtccaattccctgagcgcttcggacgtggaaaaatctaagcaagcagcgttggagctggtcaagagcttgaaaggatctccctatcgttttggtatttatacctttgcttcacactcgcctgctgctggaaacaaaaatttcacgccagtaagtcttgctaatgatgacggatacaacaaagttgttgccgctatcaatgacatccagatgccagcgatccgagagaacaaaaagggttctcccaacggtggtaccaactgggagggcgggctccaagcaatcgcgaatgacatagacagaggcatcaagtatgacgccgtttacttcatcactgatggtcaaccaacttgggataacaatgggagaaattggttgggaaccaccaccgaggttgtggaattagaaaatgccgttacccaagccaaacttatttctgataaaggcgcaaaacttattccggtgggtattggccagctttctgatgataagccgtttgatctctataaaccgattcttccttccgaagacgattactattggtcgcgttatccatggaaaatagatcgttccctgaccggcaaacaaatgctggagaagataacctcaccgggcctagagccaattattttgcccgactattccacattgccgcagcgaatgggacaacagatttttaccggatgtttccaaatcgctaaaaacattattgatgcagacggaaacgtgatagaaaatccagctggctggaatttcgatattacagcggctggtgtgcaaggcattcctccgtcgatcgagacagataaaaatggccaagacacctttgctatgaagtcgattaataaggaatcctttaagatcactatcactgagcgacctaccggagatcaaaaacaaaacttccggtttaaaaacgcacgctgccagcgctactcctatgggcaagcacctactgatattccgatcaaaactagcgatacttcaattactttgactgcagacaccaaaagcttgatttcttgcggttttaacaatctgccagttgtaccagtctcggtttcgaaaaaagtaaacgtaaatacgccacaacttttagaagagctcaacaatcaaacctttgatttcacctatagctgtgaaaaaggagctaatgaaaaagaaatcaagggagaaattaagggcgtccacaacggagaatccaaagaaatcggaaaagttgctgttggaactcagtgcgaaatcaaggaagttacccccaaagtcgacgattctcggatgaagctttccaccacttggagcagtgaaaacaccactgcagatgctaatcaggataacggtacataccgctttaaagccgacaccgatgcgtttaaaaacaagaaaacagttctagctacagcagaaaataactatgaggctcaaacagccactattaagctgaccaagaccatcattaaccgtgacaaaattccagcaacaaaactgcccgagaagtttcctgtcacttacacctgtcgttacgtaccacatcctaatgctcgccccgaacatggtgggctcccagaaaccaatccgtattttgtagactctaaaaccgttgtcgttcctcgtgatggaagtatagaaatcggaccttttccagtgggaacacagtgcagttttgaagaaactgcacgactcgatccgaatgttcaagcagacgctaaaattcctggttttagtttgaaaactgagtggaattccaacatctgttttggaaacactatcgataataattctcaagattgttctactaactcagtatggattcccaaaccaggtcaatattcgctcaacgtagaaaatacatacacgcgtgagcttgcgagcgtggagatcgaaaagacggtgagcggcgatgcctctgatctcacgaattcacacgagttttcattcaatcttcgatgtgaagattccggagtagaagtctattcgcaagacaatatcgtggtgaagaaggacggacggcaagtcatcgaaaacattcctgtcgatgccaattgtacgttgagcgaaaaacagcctgaacaaaaaggcgtggattttgtggtccccgcgccgttccatcttcgtgcttcaactgccggcgacattgtcaaagtggttgtagataacaccgcaaaacgtcaggtagctcctatttcaatacagaaaaaagttcataaaaaagacacattttctcctgaaatttctgcatcaatcgatgcattaacatacagtgtggtggcagaatgtacggttcctggtgtagaaacgcctcgaaaagttctaaaaacagtaagtgataatcaaactgttgaatttggaaactttccagtgggaactacttgtagctttagcgagctcaccgaagcccctgccggaaccgaaatgagttataaattcgcggatggtccagaggtgacaattgaggactccactcctataaataaggtgctgacgaatacgtttgaaaatgcacgtggcgagctaaaagtaaccaaaaaagtactcgatggtgatatgcctcaagcattagtagaccagattccatcgagttttacagtcaacgtcgcatgctcaatcaccggtaatcattccatcactttgcaaaaagatgagcagaaagccgtacctggggttgtt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igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z