BBa_K1378005
1
BBa_K1378005
INPNC-linker2-MVN-linker1-mRFP
2014-10-02T11:00:00Z
2015-05-08T01:10:12Z
We fused K523013, K1378005 and mRFP.
This composite part is fused by INP,MVN and mRFP.It can display MVN protein on the surface of E.coli through INP and emission red fluorecence. We use this part to detect whether MVN is translocated on the out membrane.
false
false
_1755_
0
21647
9
It's complicated
true
we set a TEV protease cutting site into linker2
false
Meng Liuyi & Zhang Zijian
annotation2407778
1
linker1
range2407778
1
1331
1360
annotation2407773
1
J23105
range2407773
1
1
35
annotation2407772
1
MVN
range2407772
1
1007
1330
annotation2407781
1
mRFP
range2407781
1
1361
2041
annotation2407782
1
B0015
range2407782
1
2048
2176
annotation2407775
1
INPNC
range2407775
1
62
985
annotation2407776
1
linker2
range2407776
1
986
1006
annotation2407774
1
B0034
range2407774
1
44
55
BBa_K1378005_sequence
1
tttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaatactagatgaccctggataaagcgctggtgctgcgcacttgtgccaataatatggccgaccattgtggcctgatctggcctgcttctggtacagttgaaagccgctattggcagtctacacgtcgtcacgaaaacgggctggttgggctgctgtggggtgccggaacatccgcctttctgagcgttcatgccgatgctcgttggattgtttgtgaggttgctgttgccgacatcattagcctggaagaaccgggtatggtgaaatttcctcgtgctgaagtggtccatgttggtgatcgtattagcgcctcccatttcatttcagctcgtcaggctgatccagcaagcacatcaacaagcacctctaccagtacactgacaccaatgccaaccgctatccctactcctatgccggctgtagcctctgttactctgcctgtggccgaacaagctcgccacgaagtctttgacgttgcctctgtgtctgctgctgctgctcctgttaacactctgcctgttaccacgcctcaaaatctgcaaacggcgacctatggctcaacactgagtggtgataaccattctcgtctgattgccgggtatggctctaacgaaaccgccggaaaccattctgatctgatcggtggtcacgattgtacgctgatggcaggtgatcaatctcgtctgaccgctggcaaaaattccgttctgaccgctggggcacgttctaaactgatcgggagcgaaggctctactctgtccgccggagaagattctaccctgatctttcgtctgtgggatggtaaacgttatcgtcagctggtcgctcgtacaggtgaaaatggtgtggaagccgacattccgtattatgtgaatgaggacgatgatattgtggacaaaccggacgaagatgatgattggatcgaagtgaaagaaaacctttatttccaaggtatgccgaacttctctcacacctgctcttctatcaactacgacccgaactctaccatcctgtctgctgaatgccaggctcgtgacggtgaatggatgccgaccgaactgcgtctgtctgaccacatcggtaacatcgacggtgaactccagttcggtgaccagaacttccaggaaacctgccaggactgccacctggagttcggtgacggtgaccagtctgtttggctggtttgcacctgccagaccatggacggtgaatggaaaccgacccagatcctgctggactctcagatcgacaacaacgactctcagctggaaatcggtggtgaaaatttgtattttcaatctggtggtatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z