BBa_K1378007 1 Binding INPNC-MVN & GFP (the Binding Plasmid) 2014-10-05T11:00:00Z 2015-05-08T01:10:12Z This is a composite part so we constructed it using various different smaller parts. INPNC comes from the existing part BBa_K523013. The sequence of MVN can be found in the genomic sequence of Microcystis aeruginosa PCC7806 and the gene was synthesized by a company (sequence can be found in the part BBa_K1378003). GFP is common and comes from the existing part BBa_E0040. This plasmid is the final testing plasmid for our E. coli- M. aeruginosa binding assay. We inserted two transcription units into one plasmid and they are expressed under the promoters J23105 and J23117 respectively. After this plasmid is transformed into E. coli, the mannan binding protein MVN (Microvirin) will be displayed on the cell surface by INPNC (Ice Nucleation Protein N and C terminal) and the E. coli cell will express GFP as well. In this way, the E. coli cells will be able to bind to M. aeruginosa cells in a "visible" way. false false _1755_ 0 20995 9 It's complicated true We optimized the sequence of MVN beforehand and chose two different promoters to avoid possible homologous recombination. false Zhang Zijian & Meng Liuyi annotation2396861 1 B0034 range2396861 1 44 55 annotation2421133 1 J23117 range2421133 1 1480 1523 annotation2421099 1 INPNC range2421099 1 62 985 annotation2421100 1 Linker1 range2421100 1 986 1015 annotation2421101 1 MVN range2421101 1 1016 1345 annotation2421136 1 B0015 range2421136 1 2281 2409 annotation2421134 1 B0030 range2421134 1 1532 1546 annotation2396862 1 J23105 range2396862 1 1 35 annotation2421102 1 B0015 range2421102 1 1352 1480 annotation2421135 1 GFP range2421135 1 1553 2272 BBa_K1378007_sequence 1 tttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaatactagatgaccctggataaagcgctggtgctgcgcacttgtgccaataatatggccgaccattgtggcctgatctggcctgcttctggtacagttgaaagccgctattggcagtctacacgtcgtcacgaaaacgggctggttgggctgctgtggggtgccggaacatccgcctttctgagcgttcatgccgatgctcgttggattgtttgtgaggttgctgttgccgacatcattagcctggaagaaccgggtatggtgaaatttcctcgtgctgaagtggtccatgttggtgatcgtattagcgcctcccatttcatttcagctcgtcaggctgatccagcaagcacatcaacaagcacctctaccagtacactgacaccaatgccaaccgctatccctactcctatgccggctgtagcctctgttactctgcctgtggccgaacaagctcgccacgaagtctttgacgttgcctctgtgtctgctgctgctgctcctgttaacactctgcctgttaccacgcctcaaaatctgcaaacggcgacctatggctcaacactgagtggtgataaccattctcgtctgattgccgggtatggctctaacgaaaccgccggaaaccattctgatctgatcggtggtcacgattgtacgctgatggcaggtgatcaatctcgtctgaccgctggcaaaaattccgttctgaccgctggggcacgttctaaactgatcgggagcgaaggctctactctgtccgccggagaagattctaccctgatctttcgtctgtgggatggtaaacgttatcgtcagctggtcgctcgtacaggtgaaaatggtgtggaagccgacattccgtattatgtgaatgaggacgatgatattgtggacaaaccggacgaagatgatgattggatcgaagtgaaaggtgaaaatttgtattttcaatctggtggtatgccgaacttctctcacacctgctcttctatcaactacgacccgaactctaccatcctgtctgctgaatgccaggctcgtgacggtgaatggatgccgaccgaactgcgtctgtctgaccacatcggtaacatcgacggtgaactccagttcggtgaccagaacttccaggaaacctgccaggactgccacctggagttcggtgacggtgaccagtctgtttggctggtttgcacctgccagaccatggacggtgaatggaaaccgacccagatcctgctggactctcagatcgacaacaacgactctcagctggaaatcggttagtagactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctagggattgtgctagctactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z