BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K1378022 1 BBa_K1378022 PelB-MlrA 2014-09-28T11:00:00Z 2015-05-08T01:10:12Z MlrA coding sequence is obtained by DNA synthesis and is fused with PelB signal peptide by PCR. MlrA coding sequence fused with PelB secretion signal peptide. false false _1755_ 0 21439 9 It's complicated false This part is used to test the secretion efficiency of MlrA by different signal peptide and thus choose a best way of secretion. false Jin Yuji annotation2389623 1 PelB range2389623 1 1 66 annotation2389624 1 MlrA range2389624 1 67 1077 BBa_K1378025 1 BBa_K1378025 PelB-MlrA-Terminator 2014-09-28T11:00:00Z 2015-05-08T01:10:12Z BBa_K1378022 and BBa_B0015 Terminator is assembled by standard assembly. MlrA coding sequence fused with PelB secretion signal peptide and terminator. false false _1755_ 0 21439 9 It's complicated false This part is used to improve the degradation efficiency of MlrA. The terminator added can make the part easier to use. false Jin Yuji component2389639 1 BBa_K1378022 component2389646 1 BBa_B0015 annotation2389646 1 BBa_B0015 range2389646 1 1086 1214 annotation2389639 1 BBa_K1378022 range2389639 1 1 1077 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1378025_sequence 1 atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatgcgtgagttcgttcgtcagcgtccgctgctgtgcttctacgttctggctatcctgatcgctctggctgctcacgctctgcgtgctatgtctccgaccccgctggacccgatgttcaaaatgcggcaggaaacccacgctcacctgaacatcatcaccgctgttcgttctaccttcgaatacccgggtgcttacaccctgctgctgttcccggctgctccgatgttcgctgctctgatcgctaccggtatcggttacggtcaggctggtttccgtgaactgctgtctcgttgcgctccgtggcgttctccggtttcttggcgtcagggtgttaccgttatcgctgtttgcttcctggctttcttcgctctgaccggtatcatgtgggttcagacctacctgtacgctccgccgggtaccctggaccgtaccttcctgcgttacggttctgacccggttgctatctacgttatgctggctgcttctctgctgctgtctccgggtccgctgctggaagaactgggttggcgtggtttcgctctgccgcagctgctgaaaaaattcgacccgctgaccgctgctgttatcctgggtatcatgtggtgggcttggcacctgccgcgtgacctgccgaccctgttctctggtgctccgggtgctgcttggtctgttatcgttaaacagctggttatcaccccgggtttcatcgcttctaccatcatcgctgttttcgtttgcaacaaactgggtggttctatgtggggtggtgttctgacccacgctatccacaacgaactgggtgttaacgttaccgctgaatgggctccgaccgttgctggtctgggttggcgtccgtgggacctgatcgagttcgctgttgctatcggtctggttctgatctgcggtcgttctctgggtgctgcttctccggacaacgctcgtctggcttggggtaacgttccgccgaaactgccgggtggtgttggtgacaaatctggtgctaacgcttgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1378022_sequence 1 atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatgcgtgagttcgttcgtcagcgtccgctgctgtgcttctacgttctggctatcctgatcgctctggctgctcacgctctgcgtgctatgtctccgaccccgctggacccgatgttcaaaatgcggcaggaaacccacgctcacctgaacatcatcaccgctgttcgttctaccttcgaatacccgggtgcttacaccctgctgctgttcccggctgctccgatgttcgctgctctgatcgctaccggtatcggttacggtcaggctggtttccgtgaactgctgtctcgttgcgctccgtggcgttctccggtttcttggcgtcagggtgttaccgttatcgctgtttgcttcctggctttcttcgctctgaccggtatcatgtgggttcagacctacctgtacgctccgccgggtaccctggaccgtaccttcctgcgttacggttctgacccggttgctatctacgttatgctggctgcttctctgctgctgtctccgggtccgctgctggaagaactgggttggcgtggtttcgctctgccgcagctgctgaaaaaattcgacccgctgaccgctgctgttatcctgggtatcatgtggtgggcttggcacctgccgcgtgacctgccgaccctgttctctggtgctccgggtgctgcttggtctgttatcgttaaacagctggttatcaccccgggtttcatcgcttctaccatcatcgctgttttcgtttgcaacaaactgggtggttctatgtggggtggtgttctgacccacgctatccacaacgaactgggtgttaacgttaccgctgaatgggctccgaccgttgctggtctgggttggcgtccgtgggacctgatcgagttcgctgttgctatcggtctggttctgatctgcggtcgttctctgggtgctgcttctccggacaacgctcgtctggcttggggtaacgttccgccgaaactgccgggtggtgttggtgacaaatctggtgctaacgcttga BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z