BBa_K1378026
1
BBa_K1378026
TorA-MlrA-Terminator
2014-09-28T11:00:00Z
2015-05-08T01:10:12Z
BBa_K1378023 and BBa_B0015 Terminator is assembled by standard assembly.
MlrA coding sequence fused with TorA secretion signal peptide and terminator.
false
false
_1755_
0
21439
9
It's complicated
false
This part is used to improve the degradation efficiency of MlrA. The terminator added can make the part easier to use.
false
Jin Yuji
component2389650
1
BBa_K1378023
component2389657
1
BBa_B0015
annotation2389657
1
BBa_B0015
range2389657
1
1152
1280
annotation2389650
1
BBa_K1378023
range2389650
1
1
1143
BBa_K1378023
1
BBa_K1378023
TorA-MlrA
2014-09-28T11:00:00Z
2015-05-08T01:10:12Z
MlrA coding sequence is obtained by DNA synthesis and is fused with TorA signal peptide by PCR.
MlrA coding sequence fused with TorA secretion signal peptide.
false
false
_1755_
0
21439
9
It's complicated
false
This part is used to test the secretion efficiency of MlrA by different signal peptide and thus choose a best way of secretion.
false
Jin Yuji
annotation2389625
1
TorA
range2389625
1
1
126
annotation2389627
1
Scar
range2389627
1
127
132
annotation2389626
1
MlrA
range2389626
1
133
1143
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1378026_sequence
1
atgaacaataacgatctctttcaggcatcacgtcggcgttttctggcacaactcggcggcttaaccgtcgccgggatgctggggccgtcattgttaacgccgcgacgtgcgactgcggcgcaagcgggatctatgcgtgagttcgttcgtcagcgtccgctgctgtgcttctacgttctggctatcctgatcgctctggctgctcacgctctgcgtgctatgtctccgaccccgctggacccgatgttcaaaatgcggcaggaaacccacgctcacctgaacatcatcaccgctgttcgttctaccttcgaatacccgggtgcttacaccctgctgctgttcccggctgctccgatgttcgctgctctgatcgctaccggtatcggttacggtcaggctggtttccgtgaactgctgtctcgttgcgctccgtggcgttctccggtttcttggcgtcagggtgttaccgttatcgctgtttgcttcctggctttcttcgctctgaccggtatcatgtgggttcagacctacctgtacgctccgccgggtaccctggaccgtaccttcctgcgttacggttctgacccggttgctatctacgttatgctggctgcttctctgctgctgtctccgggtccgctgctggaagaactgggttggcgtggtttcgctctgccgcagctgctgaaaaaattcgacccgctgaccgctgctgttatcctgggtatcatgtggtgggcttggcacctgccgcgtgacctgccgaccctgttctctggtgctccgggtgctgcttggtctgttatcgttaaacagctggttatcaccccgggtttcatcgcttctaccatcatcgctgttttcgtttgcaacaaactgggtggttctatgtggggtggtgttctgacccacgctatccacaacgaactgggtgttaacgttaccgctgaatgggctccgaccgttgctggtctgggttggcgtccgtgggacctgatcgagttcgctgttgctatcggtctggttctgatctgcggtcgttctctgggtgctgcttctccggacaacgctcgtctggcttggggtaacgttccgccgaaactgccgggtggtgttggtgacaaatctggtgctaacgcttgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1378023_sequence
1
atgaacaataacgatctctttcaggcatcacgtcggcgttttctggcacaactcggcggcttaaccgtcgccgggatgctggggccgtcattgttaacgccgcgacgtgcgactgcggcgcaagcgggatctatgcgtgagttcgttcgtcagcgtccgctgctgtgcttctacgttctggctatcctgatcgctctggctgctcacgctctgcgtgctatgtctccgaccccgctggacccgatgttcaaaatgcggcaggaaacccacgctcacctgaacatcatcaccgctgttcgttctaccttcgaatacccgggtgcttacaccctgctgctgttcccggctgctccgatgttcgctgctctgatcgctaccggtatcggttacggtcaggctggtttccgtgaactgctgtctcgttgcgctccgtggcgttctccggtttcttggcgtcagggtgttaccgttatcgctgtttgcttcctggctttcttcgctctgaccggtatcatgtgggttcagacctacctgtacgctccgccgggtaccctggaccgtaccttcctgcgttacggttctgacccggttgctatctacgttatgctggctgcttctctgctgctgtctccgggtccgctgctggaagaactgggttggcgtggtttcgctctgccgcagctgctgaaaaaattcgacccgctgaccgctgctgttatcctgggtatcatgtggtgggcttggcacctgccgcgtgacctgccgaccctgttctctggtgctccgggtgctgcttggtctgttatcgttaaacagctggttatcaccccgggtttcatcgcttctaccatcatcgctgttttcgtttgcaacaaactgggtggttctatgtggggtggtgttctgacccacgctatccacaacgaactgggtgttaacgttaccgctgaatgggctccgaccgttgctggtctgggttggcgtccgtgggacctgatcgagttcgctgttgctatcggtctggttctgatctgcggtcgttctctgggtgctgcttctccggacaacgctcgtctggcttggggtaacgttccgccgaaactgccgggtggtgttggtgacaaatctggtgctaacgcttga
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z