BBa_K1379005
type: ComponentDefinition
persistent identity: BBa K1379005
display id: BBa_K1379005
version: 1
was generated by: iGEM to SBOL conversion
description: σx Generator + PcelA-E0240
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:12Z
mutable provenance: Both comX gene and PCelA are found in genomic sequence of Streptococcus pneumoniae NCTC7465 strain, while the RBS+GFP+DTT which is downstream of PCelA is BBa_E0240. The promoter encoding comX is BBa_K880005 and the double terminator for it is BBa_B0015
owned by: james
owned by: myers
mutable description: SigmaX generator, SigmaX inducible PCelA encoding GFP
discontinued: false
dominant: false
experience: experience/None
group u list: _1756_
m user id: 0
owner id: 21235
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Jordy Evan, Nadia Benedicta, Angeline Widjaja
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_K1379006
component: BBa_E0240
component: BBa_K1379000
sequence annotation: BBa_K1379006
sequence annotation: BBa_K1379000
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K1379005_sequence/1

BBa_K1379006
type: Component
persistent identity: component2407436
display id: component2407436
version: 1
top level: BBa_K1379005
definition: ComX
access: public

BBa_E0240
type: Component
persistent identity: component2407450
display id: component2407450
version: 1
top level: BBa_K1379005
definition: GFP report
access: public

BBa_K1379000
type: Component
persistent identity: component2407439
display id: component2407439
version: 1
top level: BBa_K1379005
definition: BBa_K1379000
access: public

BBa_K1379006
type: SequenceAnnotation
persistent identity: annotation2407436
display id: annotation2407436
version: 1
top level: BBa_K1379005
location: https://synbiohub.org/public/igem/BBa_K1379005/annotation2407436/range2407436/1
component: BBa_K1379006

https://synbiohub.org/public/igem/BBa_K1379005/annotation2407436/range2407436/1
type: Range
persistent identity: range2407436
display id: range2407436
version: 1
top level: BBa_K1379005
direction: direction/reverse
start: 1
end: 711
orientation: inline

BBa_K1379000
type: SequenceAnnotation
persistent identity: annotation2407439
display id: annotation2407439
version: 1
top level: BBa_K1379005
location: https://synbiohub.org/public/igem/BBa_K1379005/annotation2407439/range2407439/1
component: BBa_K1379000

https://synbiohub.org/public/igem/BBa_K1379005/annotation2407439/range2407439/1
type: Range
persistent identity: range2407439
display id: range2407439
version: 1
top level: BBa_K1379005
direction: direction/reverse
start: 720
end: 819
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2407450
display id: annotation2407450
version: 1
top level: BBa_K1379005
location: https://synbiohub.org/public/igem/BBa_K1379005/annotation2407450/range2407450/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K1379005/annotation2407450/range2407450/1
type: Range
persistent identity: range2407450
display id: range2407450
version: 1
top level: BBa_K1379005
direction: direction/reverse
start: 828
end: 1703
orientation: inline

GFP report
type: ComponentDefinition
persistent identity: BBa E0240
display id: BBa_E0240
version: 1
was generated by: iGEM to SBOL conversion
description: GFP generator
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0032.E0040.B0015
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0032
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0240_sequence/1

BBa_B0032
type: Component
persistent identity: component1249213
display id: component1249213
version: 1
top level: GFP report
definition: BBa_B0032
access: public

BBa_B0010
type: Component
persistent identity: component1249221
display id: component1249221
version: 1
top level: GFP report
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1249216
display id: component1249216
version: 1
top level: GFP report
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component1249231
display id: component1249231
version: 1
top level: GFP report
definition: BBa_B0012
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1249213
display id: annotation1249213
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
type: Range
persistent identity: range1249213
display id: range1249213
version: 1
top level: GFP report
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249216
display id: annotation1249216
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
type: Range
persistent identity: range1249216
display id: range1249216
version: 1
top level: GFP report
direction: direction/forward
start: 20
end: 739
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249221
display id: annotation1249221
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
type: Range
persistent identity: range1249221
display id: range1249221
version: 1
top level: GFP report
direction: direction/forward
start: 748
end: 827
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249231
display id: annotation1249231
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
type: Range
persistent identity: range1249231
display id: range1249231
version: 1
top level: GFP report
direction: direction/forward
start: 836
end: 876
orientation: inline

BBa_K1379000
type: ComponentDefinition
persistent identity: BBa K1379000
display id: BBa_K1379000
version: 1
was generated by: iGEM to SBOL conversion
description: PcelA
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:12Z
mutable provenance: This promoter is found in genomic sequence of Streptococcus pneumoniae NCTC7465 strain
owned by: james
owned by: myers
mutable description: A sigmaX-dependent promoter initiating the transcription of competence CelA protein
discontinued: false
dominant: false
experience: experience/Works
group u list: _1756_
m user id: 0
owner id: 21235
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: false
creator: Ng Siu Wang Edward, Jason Jang, Joo Min Jung
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PcelA
sequence annotation: Com-Box
sequence: https://synbiohub.org/public/igem/BBa_K1379000_sequence/1

PcelA
type: SequenceAnnotation
persistent identity: annotation2386511
display id: annotation2386511
version: 1
top level: BBa_K1379000
location: https://synbiohub.org/public/igem/BBa_K1379000/annotation2386511/range2386511/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1379000/annotation2386511/range2386511/1
type: Range
persistent identity: range2386511
display id: range2386511
version: 1
top level: BBa_K1379000
direction: direction/forward
start: 1
end: 100
orientation: inline

Com-Box
type: SequenceAnnotation
persistent identity: annotation2386512
display id: annotation2386512
version: 1
top level: BBa_K1379000
location: https://synbiohub.org/public/igem/BBa_K1379000/annotation2386512/range2386512/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_K1379000/annotation2386512/range2386512/1
type: Range
persistent identity: range2386512
display id: range2386512
version: 1
top level: BBa_K1379000
direction: direction/forward
start: 74
end: 81
orientation: inline

ComX
type: ComponentDefinition
persistent identity: BBa K1379006
display id: BBa_K1379006
version: 1
was generated by: iGEM to SBOL conversion
description: S. pneumoniae σx Generator
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:12Z
mutable provenance: The protein sequence is found in genomic sequence of Streptococcus pneumoniae NCTC7465 strain while the promoter is BBa_K880005 and the double terminator is BBa_B0015
owned by: james
owned by: myers
mutable description: SigmaX Generator
discontinued: false
dominant: false
experience: experience/None
group u list: _1756_
m user id: 0
owner id: 21235
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: false
creator: Jordy Evan, Nadia Benedicta, Angeline Widjaja
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K880005
component: BBa_B0015
component: BBa_K1379004
sequence annotation: BBa_K880005
sequence annotation: BBa_K1379004
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1379006_sequence/1

BBa_K880005
type: Component
persistent identity: component2401497
display id: component2401497
version: 1
top level: ComX
definition: BBa_K880005
access: public

BBa_B0015
type: Component
persistent identity: component2401508
display id: component2401508
version: 1
top level: ComX
definition: BBa_B0015
access: public

BBa_K1379004
type: Component
persistent identity: component2401501
display id: component2401501
version: 1
top level: ComX
definition: ComX
access: public

BBa_K880005
type: SequenceAnnotation
persistent identity: annotation2401497
display id: annotation2401497
version: 1
top level: ComX
location: https://synbiohub.org/public/igem/BBa_K1379006/annotation2401497/range2401497/1
component: BBa_K880005

https://synbiohub.org/public/igem/BBa_K1379006/annotation2401497/range2401497/1
type: Range
persistent identity: range2401497
display id: range2401497
version: 1
top level: ComX
direction: direction/reverse
start: 1
end: 55
orientation: inline

BBa_K1379004
type: SequenceAnnotation
persistent identity: annotation2401501
display id: annotation2401501
version: 1
top level: ComX
location: https://synbiohub.org/public/igem/BBa_K1379006/annotation2401501/range2401501/1
component: BBa_K1379004

https://synbiohub.org/public/igem/BBa_K1379006/annotation2401501/range2401501/1
type: Range
persistent identity: range2401501
display id: range2401501
version: 1
top level: ComX
direction: direction/reverse
start: 62
end: 574
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2401508
display id: annotation2401508
version: 1
top level: ComX
location: https://synbiohub.org/public/igem/BBa_K1379006/annotation2401508/range2401508/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1379006/annotation2401508/range2401508/1
type: Range
persistent identity: range2401508
display id: range2401508
version: 1
top level: ComX
direction: direction/reverse
start: 583
end: 711
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K880005
type: ComponentDefinition
persistent identity: BBa K880005
display id: BBa_K880005
version: 1
was generated by: iGEM to SBOL conversion
description: Strong promoter, strong RBS combination for high expression levels of proteins
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:40Z
mutable provenance: It is a combination of strong promoter (J23100) and RBS (B0034).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -J23100+B0034 -Strong promoter, strong RBS combination for high expression levels of proteins Consensus constitutive promoter and RBS sequence-produces strongest possible expression.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1142_
m user id: 0
owner id: 9403
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Enter design considerations.
star: false
creator: Josh Atkinson, Mike Ferguson, and Ben Parker
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0034
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K880005_sequence/1

BBa_B0034
type: Component
persistent identity: component2204230
display id: component2204230
version: 1
top level: BBa_K880005
definition: BBa_B0034
access: public

BBa_J23100
type: Component
persistent identity: component2204228
display id: component2204228
version: 1
top level: BBa_K880005
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2204228
display id: annotation2204228
version: 1
top level: BBa_K880005
location: https://synbiohub.org/public/igem/BBa_K880005/annotation2204228/range2204228/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K880005/annotation2204228/range2204228/1
type: Range
persistent identity: range2204228
display id: range2204228
version: 1
top level: BBa_K880005
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2204230
display id: annotation2204230
version: 1
top level: BBa_K880005
location: https://synbiohub.org/public/igem/BBa_K880005/annotation2204230/range2204230/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K880005/annotation2204230/range2204230/1
type: Range
persistent identity: range2204230
display id: range2204230
version: 1
top level: BBa_K880005
direction: direction/reverse
start: 44
end: 55
orientation: inline

ComX
type: ComponentDefinition
persistent identity: BBa K1379004
display id: BBa_K1379004
version: 1
was generated by: iGEM to SBOL conversion
description: S. pneumoniae σx CDS
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:12Z
mutable provenance: The promoter is found genomic sequence of Streptococcus pneumoniae NCTC7465 strain.
owned by: james
owned by: myers
mutable description: Gene sequence of competence protein comX which produce SigmaX factor.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1756_
m user id: 0
owner id: 21235
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Jordy Evan, Nadia Benedicta, Angeline Widjaja
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: start codon
sequence annotation: comX
sequence annotation: stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1379004_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2401331
display id: annotation2401331
version: 1
top level: ComX
location: https://synbiohub.org/public/igem/BBa_K1379004/annotation2401331/range2401331/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1379004/annotation2401331/range2401331/1
type: Range
persistent identity: range2401331
display id: range2401331
version: 1
top level: ComX
direction: direction/forward
start: 1
end: 3
orientation: inline

comX
type: SequenceAnnotation
persistent identity: annotation2386502
display id: annotation2386502
version: 1
top level: ComX
location: https://synbiohub.org/public/igem/BBa_K1379004/annotation2386502/range2386502/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1379004/annotation2386502/range2386502/1
type: Range
persistent identity: range2386502
display id: range2386502
version: 1
top level: ComX
direction: direction/forward
start: 1
end: 513
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2401332
display id: annotation2401332
version: 1
top level: ComX
location: https://synbiohub.org/public/igem/BBa_K1379004/annotation2401332/range2401332/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1379004/annotation2401332/range2401332/1
type: Range
persistent identity: range2401332
display id: range2401332
version: 1
top level: ComX
direction: direction/forward
start: 507
end: 513
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

https://synbiohub.org/public/igem/BBa_K1379005_sequence/1
type: Sequence
persistent identity: BBa K1379005 sequence
display id: BBa_K1379005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgattaaagaattgtatgaagaagtccaagggactgtttataagtgtagaaatgaatattaccttcatttatgggaattgtcggattgggaccaagaaggcatgctctgcttacatgaattgattagtagagaagaaggactggtagacgatattccacgtttaaggaaatatttcaaaaccaagtttcgaaatcgaattttagactatatccgtaagcaggaaagtcagaagcgtagatacgataaagaaccctatgaagaagtgggagagatcagtcatcgtataagtgaggggggtctctggctagatgattattatctctttcatgaaacactaagagattatagaaacaaacaaagtaaagagaaacaagaagaactagaacgcgtcttaagcaatgaacgatttcgagggcgtcaaagagtattaagagacttacgcattgtgtttaaggagtttactatccgtacccacgaacaaaaactcatctcagaagaggatctgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgaccaaggaagactattttgcaaataaataagcagttgaaaagaaatttttcgactgttttttcttcctcttacgaataatctaagagaggagaaaattactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0240_sequence/1
type: Sequence
persistent identity: BBa E0240 sequence
display id: BBa_E0240_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1379000_sequence/1
type: Sequence
persistent identity: BBa K1379000 sequence
display id: BBa_K1379000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgaccaaggaagactattttgcaaataaataagcagttgaaaagaaatttttcgactgttttttcttcctcttacgaataatctaagagaggagaaaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1379006_sequence/1
type: Sequence
persistent identity: BBa K1379006 sequence
display id: BBa_K1379006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgattaaagaattgtatgaagaagtccaagggactgtttataagtgtagaaatgaatattaccttcatttatgggaattgtcggattgggaccaagaaggcatgctctgcttacatgaattgattagtagagaagaaggactggtagacgatattccacgtttaaggaaatatttcaaaaccaagtttcgaaatcgaattttagactatatccgtaagcaggaaagtcagaagcgtagatacgataaagaaccctatgaagaagtgggagagatcagtcatcgtataagtgaggggggtctctggctagatgattattatctctttcatgaaacactaagagattatagaaacaaacaaagtaaagagaaacaagaagaactagaacgcgtcttaagcaatgaacgatttcgagggcgtcaaagagtattaagagacttacgcattgtgtttaaggagtttactatccgtacccacgaacaaaaactcatctcagaagaggatctgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K880005_sequence/1
type: Sequence
persistent identity: BBa K880005 sequence
display id: BBa_K880005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1379004_sequence/1
type: Sequence
persistent identity: BBa K1379004 sequence
display id: BBa_K1379004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattaaagaattgtatgaagaagtccaagggactgtttataagtgtagaaatgaatattaccttcatttatgggaattgtcggattgggaccaagaaggcatgctctgcttacatgaattgattagtagagaagaaggactggtagacgatattccacgtttaaggaaatatttcaaaaccaagtttcgaaatcgaattttagactatatccgtaagcaggaaagtcagaagcgtagatacgataaagaaccctatgaagaagtgggagagatcagtcatcgtataagtgaggggggtctctggctagatgattattatctctttcatgaaacactaagagattatagaaacaaacaaagtaaagagaaacaagaagaactagaacgcgtcttaagcaatgaacgatttcgagggcgtcaaagagtattaagagacttacgcattgtgtttaaggagtttactatccgtacccacgaacaaaaactcatctcagaagaggatctgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z