LOCUS       BBa_K1379008            1780 bp    DNA     linear       25-Nov-2024
DEFINITION  Constitutive banana odor enzyme (ATF1) generator
ACCESSION   BBa_K1379008
VERSION     BBa_K1379008.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23102
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             63..1643
                     /label=ATF1
     misc_feature    1210..1210
                     /label=t -> c
     misc_feature    1638..1643
                     /label=double TAA stop codon
     misc_feature    476..476
                     /label=a->g to eliminate EcoRI site
     CDS             63..1643
                     /label=BBa_J45014
     stem_loop       1663..1706
                     /label=stem_loop
     misc_feature    1652..1731
                     /label=BBa_B0010
     terminator      1652..1731
                     /label=BBa_B0010
     stem_loop       1747..1766
                     /label=T7 TE
     misc_feature    1773..1773
                     /label=stop
     polyA_site      1767..1780
                     /label=polya
     misc_feature    1740..1780
                     /label=BBa_B0012
     terminator      1740..1780
                     /label=BBa_B0012
     misc_feature    44..1780
                     /label=BBa_J45219
ORIGIN
        1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagtcacaca ggaaagtact
       61 agatgaatga aatcgatgag aaaaatcagg cccccgtgca acaagaatgc ctgaaagaga
      121 tgattcagaa tgggcatgct cggcgtatgg gatctgttga agatctgtat gttgctctca
      181 acagacaaaa cttatatcga aacttctgca catatggaga attgagtgat tactgtacta
      241 gggatcagct cacattagct ttgagggaaa tctgcctgaa aaatccaact cttttacata
      301 ttgttctacc aacaagatgg ccaaatcatg aaaattatta tcgcagttcc gaatactatt
      361 cacggccaca tccagtgcat gattatattt cagtattaca agaattgaaa ctgagtggtg
      421 tggttctcaa tgaacaacct gagtacagtg cagtaatgaa gcaaatatta gaagagttca
      481 aaaatagtaa gggttcctat actgcaaaaa tttttaaact tactaccact ttgactattc
      541 cttactttgg accaacagga ccgagttggc ggctaatttg tcttccagaa gagcacacag
      601 aaaagtggaa aaaatttatc tttgtatcta atcattgcat gtctgatggt cggtcttcga
      661 tccacttttt tcatgattta agagacgaat taaataatat taaaactcca ccaaaaaaat
      721 tagattacat tttcaagtac gaggaggatt accaattatt gaggaaactt ccagaaccga
      781 tcgaaaaggt gatagacttt agaccaccgt acttgtttat tccgaagtca cttctttcgg
      841 gtttcatcta caatcatttg agattttctt caaaaggtgt ctgtatgaga atggatgatg
      901 tggaaaaaac cgatgatgtt gtcaccgaga tcatcaatat ttcaccaaca gaatttcaag
      961 cgattaaagc aaatattaaa tcaaatatcc aaggtaagtg tactatcact ccgtttttac
     1021 atgtttgttg gtttgtatct cttcataaat ggggtaaatt tttcaaacca ttgaacttcg
     1081 aatggcttac ggatattttt atccccgcag attgccgctc acaactacca gatgatgatg
     1141 aaatgagaca gatgtacaga tatggcgcta acgttggatt tattgacttc accccctgga
     1201 taagcgaatc tgacatgaat gataacaaag aaaatttttg gccacttatt gagcactacc
     1261 atgaagtaat ttcggaagct ttaagaaata aaaagcatct ccatggctta gggttcaata
     1321 tacaaggctt cgttcaaaaa tatgtgaaca ttgacaaggt aatgtgcgat cgtgccatcg
     1381 ggaaaagacg cggaggtaca ttgttaagca atgtaggtct gtttaatcag ttagaggagc
     1441 ccgatgccaa atattctata tgcgatttgg catttggcca atttcaagga tcctggcacc
     1501 aagcattttc cttgggtgtt tgttcgacta atgtaaaggg gatgaatatt gttgttgctt
     1561 caacaaagaa tgttgttggt agtcaagaat ctctcgaaga gctttgctcc atttacaaag
     1621 ctctcctttt aggcccttaa taatactaga gccaggcatc aaataaaacg aaaggctcag
     1681 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1741 cacactggct caccttcggg tgggcctttc tgcgtttata
//