##gff-version 3
##sequence-region BBa_K1379008 1 1780
BBa_K1379008	.	promoter	1	35	.	+	0	ID=BBa_J23102;Name=BBa_J23102
BBa_K1379008	.	engineered_region	44	1780	.	+	0	ID=BBa_J45219;Name=BBa_J45219
BBa_K1379008	.	terminator	1740	1780	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J45219
BBa_K1379008	.	stem_loop	1747	1766	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1379008	.	polyA_site	1767	1780	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1379008	.	stop_codon	1773	1773	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1379008	.	engineered_region	1740	1780	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1379008	.	CDS	63	1643	.	+	0	ID=BBa_J45014;Name=BBa_J45014;Parent=BBa_J45219
BBa_K1379008	.	sequence_alteration	1210	1210	.	+	0	ID=annotation1961301;Name=t -> c;Parent=BBa_J45014
BBa_K1379008	.	CDS	63	1643	.	+	0	ID=annotation1961300;Name=ATF1;Parent=BBa_J45014
BBa_K1379008	.	stop_codon	1638	1643	.	+	0	ID=annotation1961302;Name=double TAA stop codon;Parent=BBa_J45014
BBa_K1379008	.	point_mutation	476	476	.	+	0	ID=annotation1961305;Name=a->g to eliminate EcoRI site;Parent=BBa_J45014
BBa_K1379008	.	ribosome_entry_site	44	56	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J45219
BBa_K1379008	.	engineered_region	44	56	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1379008	.	ribosome_entry_site	50	53	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1379008	.	ribosome_entry_site	44	56	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1379008	.	terminator	1652	1731	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J45219
BBa_K1379008	.	engineered_region	1652	1731	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1379008	.	stem_loop	1663	1706	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K1379008
ttgacagctagctcagtcctaggtactgtgctagctactagagtcacacaggaaagtactagatgaatgaaatcgatga
gaaaaatcaggcccccgtgcaacaagaatgcctgaaagagatgattcagaatgggcatgctcggcgtatgggatctgtt
gaagatctgtatgttgctctcaacagacaaaacttatatcgaaacttctgcacatatggagaattgagtgattactgta
ctagggatcagctcacattagctttgagggaaatctgcctgaaaaatccaactcttttacatattgttctaccaacaag
atggccaaatcatgaaaattattatcgcagttccgaatactattcacggccacatccagtgcatgattatatttcagta
ttacaagaattgaaactgagtggtgtggttctcaatgaacaacctgagtacagtgcagtaatgaagcaaatattagaag
agttcaaaaatagtaagggttcctatactgcaaaaatttttaaacttactaccactttgactattccttactttggacc
aacaggaccgagttggcggctaatttgtcttccagaagagcacacagaaaagtggaaaaaatttatctttgtatctaat
cattgcatgtctgatggtcggtcttcgatccacttttttcatgatttaagagacgaattaaataatattaaaactccac
caaaaaaattagattacattttcaagtacgaggaggattaccaattattgaggaaacttccagaaccgatcgaaaaggt
gatagactttagaccaccgtacttgtttattccgaagtcacttctttcgggtttcatctacaatcatttgagattttct
tcaaaaggtgtctgtatgagaatggatgatgtggaaaaaaccgatgatgttgtcaccgagatcatcaatatttcaccaa
cagaatttcaagcgattaaagcaaatattaaatcaaatatccaaggtaagtgtactatcactccgtttttacatgtttg
ttggtttgtatctcttcataaatggggtaaatttttcaaaccattgaacttcgaatggcttacggatatttttatcccc
gcagattgccgctcacaactaccagatgatgatgaaatgagacagatgtacagatatggcgctaacgttggatttattg
acttcaccccctggataagcgaatctgacatgaatgataacaaagaaaatttttggccacttattgagcactaccatga
agtaatttcggaagctttaagaaataaaaagcatctccatggcttagggttcaatatacaaggcttcgttcaaaaatat
gtgaacattgacaaggtaatgtgcgatcgtgccatcgggaaaagacgcggaggtacattgttaagcaatgtaggtctgt
ttaatcagttagaggagcccgatgccaaatattctatatgcgatttggcatttggccaatttcaaggatcctggcacca
agcattttccttgggtgtttgttcgactaatgtaaaggggatgaatattgttgttgcttcaacaaagaatgttgttggt
agtcaagaatctctcgaagagctttgctccatttacaaagctctccttttaggcccttaataatactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttata