LOCUS BBa_K1379048 2403 bp DNA linear 22-Nov-2024 DEFINITION J23102-Lock 1-GFP-B0015-pBad/araC-Key 1-B0015 ACCESSION BBa_K1379048 VERSION BBa_K1379048.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23102 misc_feature 44..83 /label=BBa_J01010 CDS 90..809 /label=GFP protein CDS 90..809 /label=BBa_E0040 stem_loop 829..872 /label=stem_loop misc_feature 818..897 /label=BBa_B0010 terminator 818..897 /label=BBa_B0010 stem_loop 913..932 /label=T7 TE misc_feature 939..939 /label=stop polyA_site 933..946 /label=polya misc_feature 906..946 /label=BBa_B0012 terminator 906..946 /label=BBa_B0012 misc_feature 90..946 /label=BBa_I13401 promoter 2034..2164 /label=Pbad promoter 1997..2068 /label=PC misc_feature 955..2033 /label=AraC promoter 955..2164 /label=BBa_I0500 misc_feature 2173..2266 /label=BBa_J01008 stem_loop 2286..2329 /label=stem_loop misc_feature 2275..2354 /label=BBa_B0010 terminator 2275..2354 /label=BBa_B0010 stem_loop 2370..2389 /label=T7 TE misc_feature 2396..2396 /label=stop polyA_site 2390..2403 /label=polya misc_feature 2363..2403 /label=BBa_B0012 terminator 2363..2403 /label=BBa_B0012 terminator 2275..2403 /label=BBa_B0015 ORIGIN 1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagaactaga atcacctctt 61 ggatttgggt attaaagagg agatactaga tgcgtaaagg agaagaactt ttcactggag 121 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg 181 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg 241 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct 301 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag 361 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg 421 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta 481 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat 541 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca 601 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg 661 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc 721 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac 781 atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag 841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 901 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagttatga 961 caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct 1021 ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt 1081 gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat 1141 acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagatgtga 1201 cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc 1261 tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg 1321 gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca gatttatcgc 1381 cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc 1441 gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga 1501 cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata 1561 ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa 1621 aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg 1681 aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag 1741 ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc 1801 cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga 1861 agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt 1921 ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa 1981 agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga 2041 ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga 2101 tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt ttttttgggc 2161 tagctactag agacccaaat ccaggaggtg aatctagtag gtggttaatg aaaattaact 2221 tacttactag aaatatctct aaaaagccag attattaatc cggctttact agagccaggc 2281 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 2341 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 2401 ata //