BBa_K138001 1 BBa_K138001 nifHp driving mutant NifL in Leucine Landing Pad 2008-10-11T11:00:00Z 2015-05-08T01:10:13Z BBa_K138000 was created by the Michigan Team for iGEM 2008. NifL was a PCR product from pIM18. We received pIM18 form Dr. Ray Dixon, of the John Innes' Center in the United Kingdom BBa_K138000 was cut with NdeI/SpeI and ligated with a mutant form of NifL digested with NdeI/SpeI. The mutant form of NifL binds and sequesters NifA with a higher affinity than the wild-type NifL does. false false _204_ 0 3575 9 It's complicated false NifA and NifL are proteins involved in nitrogen fixation in Azobacter vinelandii. false Amrit Misra annotation1989982 1 BBa_K138000 range1989982 1 1 284 BBa_K138001_sequence 1 aacgcgttatgaagagagtcgccgcgcagcgcgccaagagattgcgtggaataagacacagggggcgacaagctgttgaacaggcgacaaagcgcccatggccccggcaggcgcaattgttctgtttcccacatttggtcgccttattgtgccgttttgttttacgtcctgcgcggcgacaaataactaacttcataaaaatcataagaatacataaacaggcacggctggtatgttccctgcacttctctgctggcaaacactcaacaacaggagaagtcacccatatgatgaccccggccaacccgaccctgagcaacgagccgcaagcgcctcacgccgagagcgacgagctgcttcccgagatctttcgccagacggtggagcatgcgcccatcgccatttccatcaccgacctcaaggccaacattctttacgccaatcgcgctttccgcaccatcaccggctacggcagcgaggaagtgctcggcaagaacgaatcgatcctctccaacggcaccacgccgcgcctggtctaccaggccctgtggggccggctggcgcagaagaagccctggtccggcgtgctggtcaaccgccgcaaggacaagaccctgtacctggccgaactgaccgtggcgccggtgctcaacgaggccggcgagaccatctactacctgggcatgcaccgcgacaccagcgaattgcacgaactggaacaacgcgtcaacaaccagcgcctgatgatcgaggcggtggtcaacgccgccccggcggcgatggtggtgctcgaccgccagcaccgggtgatgctctccaacccgagcttctgccgcctggcccgcgacctggtcgaggatggcagcagcgagagcctggtggcgctgctgcgggaaaacctcgccgcccccttcgagacgctggaaaaccagggcagcgccttctccggcaaggagatctccttcgacctgggcggccgctcgccgcgctggctgtcctgccacggccgggccatccacatcgagaacgagcaggcccacgtgttcttcgcgcccaccgaggaacgctacctgctgctgaccatcaacgacatctccgagctgcgccagaagcagcaggattcgcggctcaacgcgctgaaggcgctgatggccgaggaagagctgctgcaaggcatgcgcgagaccttcaacgccgccatccatcgcctgcagggcccggtcaacctgatcagcgcggcgatgcgcatgctcgaacggcgcctcggcgacaaggccggcaacgacccggtgctgagcgccatgcgcgaagccagcacggccggaatggaggcactggagaacctcagtggctccattccggtgcgcatggccgagtccaagatgccggtcaacctcaaccagttgatccgcgaggtgatcaccctgtgcaccgaccagttgctggcccagggcatcgtcgtcgactggcagccggcgctgcgcctgccctgggtgatgggcggggaaagcagcctgcgcagcatgatcaagcacctggtcgacaacgccatcgagtccatgagccagaaccaggtcagccgccgcgagctgttcatcagcacccgcgtggagaaccacctggtgcgcatggagatcaccgacagcggcccgggcattccgcccgacctggtgctgaaggtgttcgagccgttcttcagcaccaagccgccacaccgcgtcgggcgcggcatgggcctgccggtggtgcaggagatcgtcgccaagcacgccggcatggtgcacgtagacaccgactatcgcgaaggctgccggatcgtcgtcgagctgcccttctcggcctccacctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z