BBa_K1381007
1
BBa_K1381007
J23101-B0034-YenR
2014-10-01T11:00:00Z
2015-05-08T01:10:13Z
The source of this part is Yersinia enterocolitica.
The activator YenR coupled to the constitutive Anderson promoter J23101. YenR creates the induction of expression in the Yen system, a homologous system to the Lux system.
false
false
_1758_
0
17260
9
It's complicated
false
The coding sequence for YenR was codon optimized for E. coli.
false
Stephanie Herman, Gunta Celma, Megha Biradar, Christoffer Andersson and Martin Friberg
component2392234
1
BBa_K1381004
component2392229
1
BBa_J23101
component2392231
1
BBa_B0034
annotation2392229
1
BBa_J23101
range2392229
1
1
35
annotation2392234
1
BBa_K1381004
range2392234
1
64
816
annotation2392231
1
BBa_B0034
range2392231
1
44
55
BBa_J23101
1
BBa_J23101
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
later
Released HQ 2013
later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1381004
1
YenR
B0034-YenR
2014-10-01T11:00:00Z
2015-05-08T01:10:13Z
The source of this part is Yersinia enterocolitica.
YenR is an activator originated from Yersinia enterocolitica. It induces the expression of the yenbox_WT, the yenbox fused with a wildtype promoter from Y. enterocolitica.
Both YenR and the yenbox_WT are parts in the Yen system, which is a homolougous system to the Lux system. It is the quorum sensing system derivated from Y. enterocolitica and it senses the presence of Y. enterocoliticas unique quorum sensing molecules, the OHHL:s (3-oxo-hexanoyl homoserine lactone). When the OHHL enters the host, it interacts with YenR, making it loose its shape and thereby preventing it from binding to the yenbox. So in the presence of Y. enterocolitica the induction of the expression will be lost.
false
false
_1758_
0
17260
9
In stock
false
The coding sequence of YenR was codon optimized for E. coli.
false
Stephanie Herman, Gunta Celma, Megha Biradar, Christoffer Andersson and Martin Friberg
annotation2392210
1
YenR
range2392210
1
19
753
annotation2392209
1
B0034
range2392209
1
1
12
BBa_K1381007_sequence
1
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagagaaagaggagaaatactagatgatcattgactattttgataatgaaagcattaacgaagacattaaaaattatatccagcgccgtattaaagcgtacggcgacctgcgctactcttatctggttatgaataaaaagactccattgcacccgaccattatttctaactatccgcttgattgggttaagaaatataaaaaaaatagctaccacctgattgatcctgtaatccttaccgcgaaggataaagtggcgcctttcgcgtgggatgataactcggtgatcaataaaaaatccaccgactccgctgtttttaaactggcgcgcgagtacaatattgttaacggctacaccttcgtgttacacgataattctaacaacatggcaacactcaatattagcaacggtagcgacgacagtatctcatttgacgaatcaattgagattaacaaagagaaaattcagatgctgctgattctgactcatgaaaaaatgttgggcctgtatcaaagtaatagcgacaagaacgaaaaccgtaatccaaaaatcgaacgtgacattttcagcccgcgggaaaatgaaatcctgtactgggcctctgtgggaaaaacatatgcggaaatctcaatcatcctgggtatcaaacgctcgacagtcaaattccatattggcaacgtcgttcgtaaactcggcgtgttgaacgcgaagcatgcaatccggcttggcatcgaattgaagctgattaaacccatctaa
BBa_K1381004_sequence
1
aaagaggagaaatactagatgatcattgactattttgataatgaaagcattaacgaagacattaaaaattatatccagcgccgtattaaagcgtacggcgacctgcgctactcttatctggttatgaataaaaagactccattgcacccgaccattatttctaactatccgcttgattgggttaagaaatataaaaaaaatagctaccacctgattgatcctgtaatccttaccgcgaaggataaagtggcgcctttcgcgtgggatgataactcggtgatcaataaaaaatccaccgactccgctgtttttaaactggcgcgcgagtacaatattgttaacggctacaccttcgtgttacacgataattctaacaacatggcaacactcaatattagcaacggtagcgacgacagtatctcatttgacgaatcaattgagattaacaaagagaaaattcagatgctgctgattctgactcatgaaaaaatgttgggcctgtatcaaagtaatagcgacaagaacgaaaaccgtaatccaaaaatcgaacgtgacattttcagcccgcgggaaaatgaaatcctgtactgggcctctgtgggaaaaacatatgcggaaatctcaatcatcctgggtatcaaacgctcgacagtcaaattccatattggcaacgtcgttcgtaaactcggcgtgttgaacgcgaagcatgcaatccggcttggcatcgaattgaagctgattaaacccatctaa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_J23101_sequence
1
tttacagctagctcagtcctaggtattatgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z