LOCUS       BBa_K1381008             966 bp    DNA     linear       16-Jun-2024
DEFINITION  yenbox_WT-B0032-GFP
ACCESSION   BBa_K1381008
VERSION     BBa_K1381008.1  
FEATURES             Location/Qualifiers
     promoter        46..82
                     /label=Wildtype promoter
     misc_feature    1..48
                     /label=yenbox
     promoter        1..82
                     /label=BBa_K1381000
     RBS             91..103
                     /label=RBS-3\Weak
     RBS             97..100
                     /label=RBS
     misc_feature    91..103
                     /label=BBa_B0032
     RBS             91..103
                     /label=BBa_B0032
     CDS             110..829
                     /label=GFP protein
     CDS             110..829
                     /label=BBa_E0040
     stem_loop       849..892
                     /label=stem_loop
     misc_feature    838..917
                     /label=BBa_B0010
     terminator      838..917
                     /label=BBa_B0010
     stem_loop       933..952
                     /label=T7 TE
     misc_feature    959..959
                     /label=stop
     polyA_site      953..966
                     /label=polya
     misc_feature    926..966
                     /label=BBa_B0012
     terminator      926..966
                     /label=BBa_B0012
     misc_feature    91..966
                     /label=BBa_E0240
ORIGIN
        1 aaacctagac caaagtatag tttagataac tagacctaag gctagctatt attatacccc
       61 tatgttaatt aaactgtttt tgtactagag tcacacagga aagtactaga tgcgtaaagg
      121 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      181 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      241 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      301 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
      361 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
      421 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
      481 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
      541 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa
      601 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
      661 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca
      721 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt
      781 aacagctgct gggattacac atggcatgga tgaactatac aaataataat actagagcca
      841 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      901 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      961 tttata
//