BBa_K1381021 1 BBa_K1381021 J23116-B0034-pelB-CFY-X6 his 2014-10-06T11:00:00Z 2015-05-08T01:10:13Z Colicin FY Promotor - RBS - Export - Bacteriocin - His tag false false _1758_ 0 17571 9 It's complicated false Export tag is silver standard and Bacteriocin is freiburg so they were not compatible false Alexander Wirtanen, Niklas Handin, Marcus Hong and Jonas Mattisson component2398909 1 BBa_J32015 component2398907 1 BBa_B0034 component2398905 1 BBa_J23116 component2398910 1 BBa_K1381017 annotation2398905 1 BBa_J23116 range2398905 1 1 35 annotation2398910 1 BBa_K1381017 range2398910 1 134 1477 annotation2398907 1 BBa_B0034 range2398907 1 44 55 annotation2398909 1 BBa_J32015 range2398909 1 62 127 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J32015 1 PelB PelB leader sequence; directs protein to E. coli periplasmic membrane 2006-08-24T11:00:00Z 2015-08-31T04:08:46Z Novagen Released HQ 2013 The pelB leader sequence is a sequence of amino acids which when attached to a protein, directs the protein to the periplasmic membrane of E. coli, where the sequence is removed by pelB peptidase. It is used to direct coat protein-antigen fusions to the cell surface. false true _50_ 0 495 50 In stock true . true Austen Heinz annotation1898182 1 PelB range1898182 1 1 66 BBa_J23116 1 BBa_J23116 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Later false false _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1381017 1 CFY CFY-X6 his 2014-10-02T11:00:00Z 2015-05-08T01:10:13Z - Insert source - - Insert description - false false _1758_ 0 17260 9 In stock false - Insert design considerations - false Alexander Wirtanen, Niklas Handin, Marcus Hong and Jonas Mattisson annotation2417861 1 ATG range2417861 1 1 3 annotation2417863 1 Colicin FY range2417863 1 10 1341 annotation2417862 1 NgoMIV-site range2417862 1 4 9 annotation2417864 1 Extra TAA range2417864 1 1342 1344 BBa_J23116_sequence 1 ttgacagctagctcagtcctagggactatgctagc BBa_K1381017_sequence 1 atggccggcacggattataaagacgtggacccgtttgaacggcaggttatggaatctctgggttatgggtgggtcagtcaaggagaaggatcgtggcagaagggggaaatttcaggtgcgatcgataatgacactatgacggtaaccgggtggaacggtgccattccgacatctccttccggacgcccgcatatctgggaccagaaaattagcgagctgaacaacgcgcggcaaaacagtatcgatgacaacgacgacgacgtcacccccaccagtgatcatgaaaaaaatcaacgtcgtgaagcctcggccaaagcgttgaaatatacctcggacattgaaagcaaagtgaacgaacaattaaatgtcatcaacaatgatattcagaaaacagaggccgaatataaagaaaagcaagggggcctgtcagaagtggataaagcgaaatatcagcttgtcttatctaaactgcaattacaaaaacagaatgccaaaatcctgtccgaagaagcgaagaagcgccgcgttcacgcggaatgggaacagattaacgatgagaatgttctgaatgcccgtgatcgccctagcgcggaatggcgtcagcagcgtgaaattaacagcaaaaaaagcctcgctaaactggccgaagaacgcgctgttcaagaacaagttgaacttattcgccaggggctcgcccagctggaaaagaatattctcgccaataaagagaatgtgaaacgctggacggaggagatgaacaaaaacaaggagaaggaagagaacgaagttaaagacgcgctcaaatttaccgctgatttttacaagacggtcacggaaaagtatggcgaaaaatcctcgaaaattgcacaagaactggccgatgcagctaaaggtaaacagattcggtcagttgatgaggcgttgaaggcgttcgataagtataaagatgtcctgaataagaagttcagcgtcaaagatcgggaagctatcgctaaggcactggaaagcgttgatcgcgaactgatggccaaaaacttagcgaaattcagcaaggcgtttaattatgtaggaaaaaccattgatcgttatgataccctgaaagagattcaaaaagcgttagaaaccaacaactggcgcccagtgtttgttcaattggagtccttagccgtcggacgggccgccggtgccatcaccgctttcgccttctctatcattatcggcgcgcctcttggcattttgggcgtggccctgatcatggcattagtgtcggtgttcgtggatgatcgctttattgagaaaatcaacgaaatggtcggcgtgcaccatcaccatcaccactaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_J32015_sequence 1 atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcc BBa_K1381021_sequence 1 ttgacagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcctactagatggccggcacggattataaagacgtggacccgtttgaacggcaggttatggaatctctgggttatgggtgggtcagtcaaggagaaggatcgtggcagaagggggaaatttcaggtgcgatcgataatgacactatgacggtaaccgggtggaacggtgccattccgacatctccttccggacgcccgcatatctgggaccagaaaattagcgagctgaacaacgcgcggcaaaacagtatcgatgacaacgacgacgacgtcacccccaccagtgatcatgaaaaaaatcaacgtcgtgaagcctcggccaaagcgttgaaatatacctcggacattgaaagcaaagtgaacgaacaattaaatgtcatcaacaatgatattcagaaaacagaggccgaatataaagaaaagcaagggggcctgtcagaagtggataaagcgaaatatcagcttgtcttatctaaactgcaattacaaaaacagaatgccaaaatcctgtccgaagaagcgaagaagcgccgcgttcacgcggaatgggaacagattaacgatgagaatgttctgaatgcccgtgatcgccctagcgcggaatggcgtcagcagcgtgaaattaacagcaaaaaaagcctcgctaaactggccgaagaacgcgctgttcaagaacaagttgaacttattcgccaggggctcgcccagctggaaaagaatattctcgccaataaagagaatgtgaaacgctggacggaggagatgaacaaaaacaaggagaaggaagagaacgaagttaaagacgcgctcaaatttaccgctgatttttacaagacggtcacggaaaagtatggcgaaaaatcctcgaaaattgcacaagaactggccgatgcagctaaaggtaaacagattcggtcagttgatgaggcgttgaaggcgttcgataagtataaagatgtcctgaataagaagttcagcgtcaaagatcgggaagctatcgctaaggcactggaaagcgttgatcgcgaactgatggccaaaaacttagcgaaattcagcaaggcgtttaattatgtaggaaaaaccattgatcgttatgataccctgaaagagattcaaaaagcgttagaaaccaacaactggcgcccagtgtttgttcaattggagtccttagccgtcggacgggccgccggtgccatcaccgctttcgccttctctatcattatcggcgcgcctcttggcattttgggcgtggccctgatcatggcattagtgtcggtgttcgtggatgatcgctttattgagaaaatcaacgaaatggtcggcgtgcaccatcaccatcaccactaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z