BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_J32015
1
PelB
PelB leader sequence; directs protein to E. coli periplasmic membrane
2006-08-24T11:00:00Z
2015-08-31T04:08:46Z
Novagen
Released HQ 2013
The pelB leader sequence is a sequence of amino acids which when attached to a protein, directs the protein to the periplasmic membrane of E. coli, where the sequence is removed by pelB peptidase. It is used to direct coat protein-antigen fusions to the cell surface.
false
true
_50_
0
495
50
In stock
true
.
true
Austen Heinz
annotation1898182
1
PelB
range1898182
1
1
66
BBa_K1381017
1
CFY
CFY-X6 his
2014-10-02T11:00:00Z
2015-05-08T01:10:13Z
- Insert source -
- Insert description -
false
false
_1758_
0
17260
9
In stock
false
- Insert design considerations -
false
Alexander Wirtanen, Niklas Handin, Marcus Hong and Jonas Mattisson
annotation2417863
1
Colicin FY
range2417863
1
10
1341
annotation2417861
1
ATG
range2417861
1
1
3
annotation2417864
1
Extra TAA
range2417864
1
1342
1344
annotation2417862
1
NgoMIV-site
range2417862
1
4
9
BBa_J23116
1
BBa_J23116
constitutive promoter family member
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
Later
Later
false
false
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K1381021
1
BBa_K1381021
J23116-B0034-pelB-CFY-X6 his
2014-10-06T11:00:00Z
2015-05-08T01:10:13Z
Colicin FY
Promotor - RBS - Export - Bacteriocin - His tag
false
false
_1758_
0
17571
9
It's complicated
false
Export tag is silver standard and Bacteriocin is freiburg so they were not compatible
false
Alexander Wirtanen, Niklas Handin, Marcus Hong and Jonas Mattisson
component2398907
1
BBa_B0034
component2398905
1
BBa_J23116
component2398910
1
BBa_K1381017
component2398909
1
BBa_J32015
annotation2398907
1
BBa_B0034
range2398907
1
44
55
annotation2398909
1
BBa_J32015
range2398909
1
62
127
annotation2398910
1
BBa_K1381017
range2398910
1
134
1477
annotation2398905
1
BBa_J23116
range2398905
1
1
35
BBa_J23116_sequence
1
ttgacagctagctcagtcctagggactatgctagc
BBa_K1381017_sequence
1
atggccggcacggattataaagacgtggacccgtttgaacggcaggttatggaatctctgggttatgggtgggtcagtcaaggagaaggatcgtggcagaagggggaaatttcaggtgcgatcgataatgacactatgacggtaaccgggtggaacggtgccattccgacatctccttccggacgcccgcatatctgggaccagaaaattagcgagctgaacaacgcgcggcaaaacagtatcgatgacaacgacgacgacgtcacccccaccagtgatcatgaaaaaaatcaacgtcgtgaagcctcggccaaagcgttgaaatatacctcggacattgaaagcaaagtgaacgaacaattaaatgtcatcaacaatgatattcagaaaacagaggccgaatataaagaaaagcaagggggcctgtcagaagtggataaagcgaaatatcagcttgtcttatctaaactgcaattacaaaaacagaatgccaaaatcctgtccgaagaagcgaagaagcgccgcgttcacgcggaatgggaacagattaacgatgagaatgttctgaatgcccgtgatcgccctagcgcggaatggcgtcagcagcgtgaaattaacagcaaaaaaagcctcgctaaactggccgaagaacgcgctgttcaagaacaagttgaacttattcgccaggggctcgcccagctggaaaagaatattctcgccaataaagagaatgtgaaacgctggacggaggagatgaacaaaaacaaggagaaggaagagaacgaagttaaagacgcgctcaaatttaccgctgatttttacaagacggtcacggaaaagtatggcgaaaaatcctcgaaaattgcacaagaactggccgatgcagctaaaggtaaacagattcggtcagttgatgaggcgttgaaggcgttcgataagtataaagatgtcctgaataagaagttcagcgtcaaagatcgggaagctatcgctaaggcactggaaagcgttgatcgcgaactgatggccaaaaacttagcgaaattcagcaaggcgtttaattatgtaggaaaaaccattgatcgttatgataccctgaaagagattcaaaaagcgttagaaaccaacaactggcgcccagtgtttgttcaattggagtccttagccgtcggacgggccgccggtgccatcaccgctttcgccttctctatcattatcggcgcgcctcttggcattttgggcgtggccctgatcatggcattagtgtcggtgttcgtggatgatcgctttattgagaaaatcaacgaaatggtcggcgtgcaccatcaccatcaccactaataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_J32015_sequence
1
atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcc
BBa_K1381021_sequence
1
ttgacagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcctactagatggccggcacggattataaagacgtggacccgtttgaacggcaggttatggaatctctgggttatgggtgggtcagtcaaggagaaggatcgtggcagaagggggaaatttcaggtgcgatcgataatgacactatgacggtaaccgggtggaacggtgccattccgacatctccttccggacgcccgcatatctgggaccagaaaattagcgagctgaacaacgcgcggcaaaacagtatcgatgacaacgacgacgacgtcacccccaccagtgatcatgaaaaaaatcaacgtcgtgaagcctcggccaaagcgttgaaatatacctcggacattgaaagcaaagtgaacgaacaattaaatgtcatcaacaatgatattcagaaaacagaggccgaatataaagaaaagcaagggggcctgtcagaagtggataaagcgaaatatcagcttgtcttatctaaactgcaattacaaaaacagaatgccaaaatcctgtccgaagaagcgaagaagcgccgcgttcacgcggaatgggaacagattaacgatgagaatgttctgaatgcccgtgatcgccctagcgcggaatggcgtcagcagcgtgaaattaacagcaaaaaaagcctcgctaaactggccgaagaacgcgctgttcaagaacaagttgaacttattcgccaggggctcgcccagctggaaaagaatattctcgccaataaagagaatgtgaaacgctggacggaggagatgaacaaaaacaaggagaaggaagagaacgaagttaaagacgcgctcaaatttaccgctgatttttacaagacggtcacggaaaagtatggcgaaaaatcctcgaaaattgcacaagaactggccgatgcagctaaaggtaaacagattcggtcagttgatgaggcgttgaaggcgttcgataagtataaagatgtcctgaataagaagttcagcgtcaaagatcgggaagctatcgctaaggcactggaaagcgttgatcgcgaactgatggccaaaaacttagcgaaattcagcaaggcgtttaattatgtaggaaaaaccattgatcgttatgataccctgaaagagattcaaaaagcgttagaaaccaacaactggcgcccagtgtttgttcaattggagtccttagccgtcggacgggccgccggtgccatcaccgctttcgccttctctatcattatcggcgcgcctcttggcattttgggcgtggccctgatcatggcattagtgtcggtgttcgtggatgatcgctttattgagaaaatcaacgaaatggtcggcgtgcaccatcaccatcaccactaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z