BBa_K1381024 1 BBa_K1381024 B0034-PgsA-(EAAAR)x4 2014-10-06T11:00:00Z 2015-05-08T01:10:13Z PgsA gene from Bacillus Subtilis Anchor tag to make fusion protein for cell surface display false false _1758_ 0 17571 9 It's complicated false Designed with freiburg fusion standard but ony got it to work by using normal E, X, S, P restriction false Viktor Blomkvist, Tim Hagelby Edstrom, Laura Pacoste and Tersea Reinli annotation2417832 1 TAA stop range2417832 1 1225 1227 annotation2417828 1 PgsA range2417828 1 19 1158 annotation2417827 1 B0034 range2417827 1 1 12 annotation2417829 1 TACTAG range2417829 1 13 18 annotation2417831 1 AgeI-site range2417831 1 1219 1224 annotation2417830 1 (EAAAR)x4 range2417830 1 1159 1218 BBa_K1381024_sequence 1 aaagaggagaaatactagatgaagaaagagctcagcttccacgagaaactgttaaagttaacgaaacagcagaaaaagaaaactaataagcatgtatttattgccattccgattgtttttgttctgatgttcgctttcatgtgggcgggtaaagccgaaaccccaaaagttaaaacgtatagcgacgacgttctgagcgcatcattcgtgggagacattatgatggggcgttacgtcgaaaaagttacagaacaaaaaggtgccgattccatttttcagtatgtggagcctatcttccgtgcaagtgattatgtggccggtaacttcgaaaacccggtaacgtaccagaaaaactataaacaggccgataaagagatccatttacaaactaacaaagagtcagttaaagtgctcaaagatatgaactttactgtgttaaattctgcaaacaaccacgcaatggattatggtgttcaggggatgaaggataccttgggcgagtttgcgaaacagaacctggacatcgttggtgctggttatagtttgtccgatgccaagaagaaaatttcataccagaaggtgaatggcgtgaccattgccaccttggggttcacggacgtaagcggcaaaggattcgcagcaaaaaagaacacgccgggggtcttgcccgcggacccagaaatttttatcccgatgatttcggaggcaaaaaaacatgcggatattgtggttgttcaaagccattggggccaggaatatgataatgatccgaatgatcgtcaacggcagttggcgcgtgctatgtctgacgccggtgcggacattattgtgggccatcatccacacgtattggagccaattgaagtttataatggtacggtgatcttctactctctggggaacttcgtttttgatcaaggctggacgcgcacccgtgacagcgccctggtccaataccacctgaaaaaaaacggcacaggtcgttttgaggtaaccccgatcgatattcatgaagcgacacctgcgccagtaaaaaaagactccttgaagcagaagaccattatccgtgaactgaccaaagattctaacttcgcgtggaaagtggaggacggaaagctgacatttgatattgatcacagtgacaagctgaagagcaaagaagctgcggctcgcgaggcggctgcgcgcgaggcggcggcccgtgaggcggcagcacgtaccggttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z