BBa_K1382001 1 BBa_K1382001 GST-melittin 2014-06-22T11:00:00Z 2015-05-08T01:10:13Z Melittin- Apis Mellifera This consists of melittin fused to GST (Glutathione S-Tranferase) with a TEV cleavage site. Melittin is a toxin which causes cell death. GST-melittin is an inactive form of melittin, which ensures that melittin can be produced in a cell without killing the host cell. To activate melittin, TEV protease is added to cleave melittin from GST. false false _1759_ 0 21714 9 It's complicated false Thrombin was the initial cleavage site, however we decided to use TEV because it was more efficient. As a result, we have both thrombin and TEV cleavage sites. false Maryam Ige, Janki Patel annotation2379463 1 Melittin range2379463 1 709 792 annotation2379461 1 GST range2379461 1 1 654 annotation2379460 1 Thrombin cleavage site range2379460 1 661 678 annotation2379462 1 TEV cleavage site range2379462 1 685 705 BBa_K1382001_sequence 1 atgtcccctatactaggttattggaaaattaagggccttgtgcaacccactcgacttcttttggaatatcttgaagaaaaatatgaagagcatttgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattgggtttggagtttcccaatcttccttattatattgatggtgatgttaaattaacacagtctatggccatcatacgttatatagctgacaagcacaacatgttgggtggttgtccaaaagagcgtgcagagatttcaatgcttgaaggagcggttttggatattagatacggtgtttcgagaattgcatatagtaaagactttgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaatgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgtaacccatcctgacttcatgttgtatgacgctcttgatgttgttttatacatggacccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaacgtattgaagctatcccacaaattgataagtacttgaaatccagcaagtatatagcatggcctttgcagggctggcaagccacgtttggtggtggcgaccatcctccaaaatcggatctggttccgcgtggatcgcatatggagaatctttattttcagggcatgggaattggagcagttctgaaggtattaaccacaggattgcccgccctcataagttggattaaacgtaagaggcaacagggttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z