BBa_K1382001
1
BBa_K1382001
GST-melittin
2014-06-22T11:00:00Z
2015-05-08T01:10:13Z
Melittin- Apis Mellifera
This consists of melittin fused to GST (Glutathione S-Tranferase) with a TEV cleavage site. Melittin is a toxin which causes cell death. GST-melittin is an inactive form of melittin, which ensures that melittin can be produced in a cell without killing the host cell. To activate melittin, TEV protease is added to cleave melittin from GST.
false
false
_1759_
0
21714
9
It's complicated
false
Thrombin was the initial cleavage site, however we decided to use TEV because it was more efficient. As a result, we have both thrombin and TEV cleavage sites.
false
Maryam Ige, Janki Patel
annotation2379460
1
Thrombin cleavage site
range2379460
1
661
678
annotation2379461
1
GST
range2379461
1
1
654
annotation2379463
1
Melittin
range2379463
1
709
792
annotation2379462
1
TEV cleavage site
range2379462
1
685
705
BBa_K1382001_sequence
1
atgtcccctatactaggttattggaaaattaagggccttgtgcaacccactcgacttcttttggaatatcttgaagaaaaatatgaagagcatttgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattgggtttggagtttcccaatcttccttattatattgatggtgatgttaaattaacacagtctatggccatcatacgttatatagctgacaagcacaacatgttgggtggttgtccaaaagagcgtgcagagatttcaatgcttgaaggagcggttttggatattagatacggtgtttcgagaattgcatatagtaaagactttgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaatgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgtaacccatcctgacttcatgttgtatgacgctcttgatgttgttttatacatggacccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaacgtattgaagctatcccacaaattgataagtacttgaaatccagcaagtatatagcatggcctttgcagggctggcaagccacgtttggtggtggcgaccatcctccaaaatcggatctggttccgcgtggatcgcatatggagaatctttattttcagggcatgggaattggagcagttctgaaggtattaaccacaggattgcccgccctcataagttggattaaacgtaagaggcaacagggttaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z