LOCUS       BBa_K1385001             957 bp    DNA     linear       05-Nov-2024
DEFINITION  Hybrid CpcG2 promoter -> TetR
ACCESSION   BBa_K1385001
VERSION     BBa_K1385001.1  
FEATURES             Location/Qualifiers
     promoter        1..174
                     /label=Truncated cpcG2 Promoter
     misc_feature    105..126
                     /label=G-Box (CcaR binding site)
     misc_feature    175..177
                     /label=ACC Replaced with AAA Spacer
     promoter        178..240
                     /label=trc1O Promoter
     misc_feature    215..231
                     /label=Lac Operator site
     CDS             329..952
                     /label=TetR 
     misc_feature    950..952
                     /label=Stop Codon
     misc_feature    329..331
                     /label=Start Codon
     misc_feature    213..213
                     /label=TSS (+1)
ORIGIN
        1 agcccattgt gcttttctct atcaacctca gcttacctga aggggtgaac aggtctgggt
       61 taattcatgt tgcgaaatgt aacagtttta gtcgcatcag ctaactttcc gatttcttta
      121 cgattttctc ccccttttct tcaattttac tttgttagga tcgcattttt aatgaaattg
      181 acaattaatc atccggctcg tataatgtgt ggaattgtga gcggataaca atttcacaca
      241 atgatagatc ccgtcgtttt acaacgtcgt gactgggaag gctagctcag tcctaggtac
      301 agtgctagct cacacaggaa aggcctcgat gtccagatta gataaaagta aagtgattaa
      361 cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc
      421 ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt
      481 gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt gccctttaga
      541 aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact
      601 aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga
      661 aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt
      721 atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca
      781 tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg
      841 acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga
      901 attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtcct aataatc
//