LOCUS       BBa_K1387006            1567 bp    DNA     linear       23-Dec-2024
DEFINITION  J23106 - tetO - B0034 - lpp ompA-LC-Cutinase - B1002
ACCESSION   BBa_K1387006
VERSION     BBa_K1387006.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     promoter        44..58
                     /label=BBa_K079036
     misc_feature    71..74
                     /label=conserved
     RBS             67..78
                     /label=BBa_B0034
     misc_feature    87..93
                     /label=NdeI
     CDS             90..524
                     /label=OmpA
     CDS             525..550
                     /label=linker
     misc_feature    545..550
                     /label=SacI
     misc_feature    90..92
                     /label=ATG
     misc_feature    87..550
                     /label=BBa_K103006
     misc_feature    557..622
                     /label=pelB
     CDS             623..1501
                     /label=LC-Cutinase
     misc_feature    1502..1519
                     /label=Polyhistidine-tag
     CDS             557..1525
                     /label=BBa_K936014
     misc_feature    1534..1567
                     /label=B1002
     stem_loop       1543..1558
                     /label=stem loop
     polyA_site      1558..1564
                     /label=Poly A tail
     polyA_site      1537..1542
                     /label=Poly A tail
     terminator      1534..1567
                     /label=BBa_B1002
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagcctatca gtgatagata
       61 ctagagaaag aggagaaata ctagagcata tgaaagctac taaactggta ctgggcgcgg
      121 taatcctggg ttctactctg ctggcaggtt gctccagcaa cgctaaaatc gatcagggaa
      181 ttaacccgta tgttggcttt gaaatgggtt acgactggtt aggtcgtatg ccgtacaaag
      241 gcagcgttga aaacggtgca tacaaagctc agggcgttca actgaccgct aaactgggtt
      301 acccaatcac tgacgacctg gacatctaca ctcgtctggg tggcatggta tggcgtgcag
      361 acactaaatc caacgtttat ggtaaaaacc acgacaccgg cgtttctccg gtcttcgctg
      421 gcggtgttga gtacgcgatc actcctgaaa tcgctacccg tctggaatac cagtggacca
      481 acaacatcgg tgacgcacac accatcggca ctcgtccgga caacggcgga ggttctggag
      541 gagggagctc tactagatga aatacctgct gccgaccgct gctgctggtc tgctgctcct
      601 cgctgcccag ccggcgatgg ccatggacgg agttctctgg cgagtgcgaa ccgcggcgct
      661 catggccgcg ctgctcgccc tcgcagcctg ggcgctggtc tgggcttcgc ccagcgtcga
      721 ggctcaatcc aacccgtacc agcgcggtcc caaccccacg cggagcgcgc tcacggccga
      781 cgggccgttc tcggtggcaa cctacaccgt ttcgcggctc tcggtgagcg gcttcggggg
      841 cggggtgatc tactacccca caggcacctc gctgaccttc ggcgggatcg ccatgtcgcc
      901 ggggtacacg gccgacgcca gttcgctggc gtggctcggg cggcggttgg catcgcacgg
      961 cttcgtggtg ctcgtcatca acacgaactc gcgattcgac taccctgact cccgcgcaag
     1021 ccagctatcg gcggcgctga actacctgcg gacgagcagc ccctcagccg tacgcgcccg
     1081 gctcgatgcg aaccgcctgg ccgttgcggg gcattcgatg ggcggcggcg ggaccctccg
     1141 catcgcagag cagaacccgt cgctgaaagc ggccgtaccg ctcacgccgt ggcacaccga
     1201 caagacgttc aacacgtcgg taccggtgct catcgtggga gcggaagcgg acacggtcgc
     1261 gcccgtgagc cagcacgcca ttccgttcta ccagaacttg ccctcgacca cgccgaaggt
     1321 gtacgtggag ctcgacaatg cgtcgcactt cgcgcccaac agcaacaacg cggcgatctc
     1381 cgtgtacacc atctcgtgga tgaagctgtg ggtggataac gacacccgct accggcagtt
     1441 cctctgcaac gtgaacgatc cggcgctgag cgacttccgg acgaacaacc gccactgcca
     1501 gcatcaccac catcaccatt agtaatacta gagcgcaaaa aaccccgctt cggcggggtt
     1561 ttttcgc
//