BBa_K936014
1
BBa_K936014
pelB-LC-Cutinase with polyhistidine-tag
2012-08-08T11:00:00Z
2015-05-08T01:13:47Z
This part was created from other BioBrick parts.
pelB leader sequence attached to enzyme LC-Cutinase protein coding sequence in order to allow the cell to bring the enzyme to the periplasm for easier secretion. The polyhistidine-tag allows for us to purify the produced enzyme.
false
true
_1201_
0
10185
9
Not in stock
false
We designed the pelB tag to be immediately in front of the LC-cutinase gene.
false
Nicholas Csicsery
annotation2181454
1
pelB
range2181454
1
1
66
annotation2181456
1
Polyhistidine-tag
range2181456
1
946
963
annotation2181455
1
LC-Cutinase
range2181455
1
67
945
BBa_B1002
1
BBa_B1002
Terminator (artificial, small, %T~=85%)
2006-08-29T11:00:00Z
2015-08-31T04:07:21Z
antiquity
Artifical terminator, estimated %T~=85
false
true
_41_
0
745
41
In stock
false
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues.
true
Haiyao Huang
annotation1898412
1
B1002
range1898412
1
1
34
annotation1898414
1
Poly A tail
range1898414
1
25
31
annotation1898415
1
Poly A tail
range1898415
1
4
9
annotation1898413
1
stem loop
range1898413
1
10
25
BBa_K1387006
1
BBa_K1387006
J23106 - tetO - B0034 - lpp ompA-LC-Cutinase - B1002
2014-09-28T11:00:00Z
2015-05-08T01:10:13Z
This part, we desgned through synthetic gene (G-Blocks)
The casette of degradation module is comprised of constitutive promoter, tet operator, RBS, outer membrane protein A (ompA) fused with LC Cutinase, and double terminator. By using constitutive promoter, we expect the recombinant protein, in this case ompA-LC Cutinase, will be synthesized indefinitely. ompA is outer membrane protein. It comprises of β???strand B3-B7. Rather than using full sequence of ompA, we use ompA (46-159) fused with lipoprotein signal sequence, thus exposing it on the cell surface of bacteria1. We fuse lpp-ompA with LC Cutinase, an enzyme that exhibit esterase activity. LC-Cutinase esterase activity will cleave the ester bond on PET and releasing terephthalic acid and ethylene glycol as a product. Fusion strategy of lpp-ompA on LC Cutinase gene is one of our way to create a whole cell biocatalyst which will be an effective molecular machinery to degrade PET on the environment. Due to constitutive expression of the recombinant protein in E.coli, there is a possibility of formation of insoluble protein inside the cell (inclusion body), because of that, we put tet operator upstream of RBS for further regulation.
false
false
_1764_
0
20698
9
It's complicated
false
Fusion strategy of lpp-ompA on LC Cutinase gene is one of our way to create a whole cell biocatalyst
false
Tri Ekawati Heryanto, Joko Pebrianto Trinugroho, Pande Putu Erawijantari, Nimas Ghasani, Risma Wiharyanti, Gilang Tirta Widi, Oktira Roka Aji
component2388102
1
BBa_K103006
component2388093
1
BBa_J23106
component2388096
1
BBa_B0034
component2388111
1
BBa_B1002
component2388106
1
BBa_K936014
component2388094
1
BBa_K079036
annotation2388102
1
BBa_K103006
range2388102
1
87
550
annotation2388094
1
BBa_K079036
range2388094
1
44
58
annotation2388111
1
BBa_B1002
range2388111
1
1534
1567
annotation2388096
1
BBa_B0034
range2388096
1
67
78
annotation2388106
1
BBa_K936014
range2388106
1
557
1525
annotation2388093
1
BBa_J23106
range2388093
1
1
35
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K103006
1
OmpA
OmpA outer membrane protein A fused to linker; displays proteins on cell surface
2008-09-13T11:00:00Z
2015-05-08T01:08:46Z
One of our basic bricks used to create fusions attached to outer membrane
false
true
_180_
0
2582
9
In stock
true
true
Michael Lower
annotation1990001
1
linker
range1990001
1
439
464
annotation1990000
1
OmpA
range1990000
1
4
438
annotation1989999
1
NdeI
range1989999
1
1
7
annotation1990002
1
SacI
range1990002
1
459
464
annotation1993480
1
ATG
range1993480
1
4
6
BBa_K079036
1
BBa_K079036
Tet O operator library member
2008-10-24T11:00:00Z
2015-05-08T01:08:34Z
..
..
false
false
_185_
0
3530
9
Not in stock
true
..
false
Francesca Ceroni
BBa_J23106
1
BBa_J23106
constitutive promoter family member
2006-08-13T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K103006_sequence
1
catatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctc
BBa_B1002_sequence
1
cgcaaaaaaccccgcttcggcggggttttttcgc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K079036_sequence
1
cctatcagtgataga
BBa_K936014_sequence
1
atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatggacggagttctctggcgagtgcgaaccgcggcgctcatggccgcgctgctcgccctcgcagcctgggcgctggtctgggcttcgcccagcgtcgaggctcaatccaacccgtaccagcgcggtcccaaccccacgcggagcgcgctcacggccgacgggccgttctcggtggcaacctacaccgtttcgcggctctcggtgagcggcttcgggggcggggtgatctactaccccacaggcacctcgctgaccttcggcgggatcgccatgtcgccggggtacacggccgacgccagttcgctggcgtggctcgggcggcggttggcatcgcacggcttcgtggtgctcgtcatcaacacgaactcgcgattcgactaccctgactcccgcgcaagccagctatcggcggcgctgaactacctgcggacgagcagcccctcagccgtacgcgcccggctcgatgcgaaccgcctggccgttgcggggcattcgatgggcggcggcgggaccctccgcatcgcagagcagaacccgtcgctgaaagcggccgtaccgctcacgccgtggcacaccgacaagacgttcaacacgtcggtaccggtgctcatcgtgggagcggaagcggacacggtcgcgcccgtgagccagcacgccattccgttctaccagaacttgccctcgaccacgccgaaggtgtacgtggagctcgacaatgcgtcgcacttcgcgcccaacagcaacaacgcggcgatctccgtgtacaccatctcgtggatgaagctgtgggtggataacgacacccgctaccggcagttcctctgcaacgtgaacgatccggcgctgagcgacttccggacgaacaaccgccactgccagcatcaccaccatcaccattagtaa
BBa_J23106_sequence
1
tttacggctagctcagtcctaggtatagtgctagc
BBa_K1387006_sequence
1
tttacggctagctcagtcctaggtatagtgctagctactagagcctatcagtgatagatactagagaaagaggagaaatactagagcatatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctctactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatggacggagttctctggcgagtgcgaaccgcggcgctcatggccgcgctgctcgccctcgcagcctgggcgctggtctgggcttcgcccagcgtcgaggctcaatccaacccgtaccagcgcggtcccaaccccacgcggagcgcgctcacggccgacgggccgttctcggtggcaacctacaccgtttcgcggctctcggtgagcggcttcgggggcggggtgatctactaccccacaggcacctcgctgaccttcggcgggatcgccatgtcgccggggtacacggccgacgccagttcgctggcgtggctcgggcggcggttggcatcgcacggcttcgtggtgctcgtcatcaacacgaactcgcgattcgactaccctgactcccgcgcaagccagctatcggcggcgctgaactacctgcggacgagcagcccctcagccgtacgcgcccggctcgatgcgaaccgcctggccgttgcggggcattcgatgggcggcggcgggaccctccgcatcgcagagcagaacccgtcgctgaaagcggccgtaccgctcacgccgtggcacaccgacaagacgttcaacacgtcggtaccggtgctcatcgtgggagcggaagcggacacggtcgcgcccgtgagccagcacgccattccgttctaccagaacttgccctcgaccacgccgaaggtgtacgtggagctcgacaatgcgtcgcacttcgcgcccaacagcaacaacgcggcgatctccgtgtacaccatctcgtggatgaagctgtgggtggataacgacacccgctaccggcagttcctctgcaacgtgaacgatccggcgctgagcgacttccggacgaacaaccgccactgccagcatcaccaccatcaccattagtaatactagagcgcaaaaaaccccgcttcggcggggttttttcgc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z