LOCUS       BBa_K1388001            1298 bp    DNA     linear       29-Nov-2024
DEFINITION  aeBlue-aacC1 gene cassette for integron testing
ACCESSION   BBa_K1388001
VERSION     BBa_K1388001.1  
FEATURES             Location/Qualifiers
     primer_bind     1..27
                     /label=Circularisation spacer region
     misc_feature    34..93
                     /label=attC recombination site
     RBS             95..102
                     /label=rbs (aeBlue)
     CDS             110..808
                     /label=aeBlue
     RBS             817..823
                     /label=rbs (aacC1)
     CDS             831..1298
                     /label=aacC1 (GmR)
ORIGIN
        1 taccgaggga cagactacca actcacagct agcgcctaac aattcgtcca agccgacgcc
       61 gcttcgcggc gcggcttaac tcaggtgtta ggcaaaggag gttattgata tggcttcact
      121 ggttaaaaaa gacatgtgca tcaaaatgac gatggaagga acagtaaacg gtcaccattt
      181 caagtgtgta ggagaaggcg aaggcaaacc atttgaaggg acccaggtgg aaaagatacg
      241 catcactgaa ggtgggccct taccatttgc gtatgatatt ttggcccctt gttgcatgta
      301 tggcagtaaa accttcatta agcatgtgtc gggtattccg gattacttta aggagtcttt
      361 tcctgagggc tttacctggg aaagaacaca aatcttcgag gatggcggct atctcaccat
      421 acaccaggac acgagccttc agggtaataa ttttattttc aaagttaatg tcatcggtgc
      481 caacttccct gcaaacggtc ccgtgatgca gaaaaaaaca gctggatggg aaccgtgcgt
      541 tgagatgctt tatccgcggg acggcgtcct gtgtggtcag agcctgatgg ccctgaaatg
      601 cactgatggc aatcatctga cgtcccacct gcgcactacc tatcgttctc gcaagccatc
      661 caatgcagtt aacatgccgg aatttcattt tggggatcat cgcattgaga ttttgaaagc
      721 tgaacaaggt aaattttatg aacaatacga gtcagcggtg gcccgttact gtgaggcggc
      781 accgagtaaa ttagggcatc actaataaag gatccaaagg aggctcaagt atgggcatca
      841 ttcgcacatg taggctcggc cctgaccaag tcaaatccat gcgggctgct cttgatcttt
      901 tcggtcgtga gttcggagac gtagccacct actcccaaca tcagccggac tccgattacc
      961 tcgggaactt gctccgtagt aagacattca tcgcgcttgc tgccttcgac caagaagcgg
     1021 ttgttggcgc tctcgcggct tacgttctgc ccaggtttga gcagccgcgt agtgagatct
     1081 atatctatga tctcgcagtc tccggcgagc accggaggca gggcattgcc accgcgctca
     1141 tcaatctcct caagcatgag gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag
     1201 attacggtga cgatcccgca gtggctctct atacaaagtt gggcatacgg gaagaagtga
     1261 tgcactttga tatcgaccca agtaccgcca cctaataa
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