LOCUS BBa_K1390019 5403 bp DNA linear 25-Nov-2024 DEFINITION Complete sMMO of Methylococcus capsulatus ACCESSION BBa_K1390019 VERSION BBa_K1390019.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 RBS 64..76 /label=RBS-3\Weak RBS 70..73 /label=RBS misc_feature 64..76 /label=BBa_B0032 RBS 64..76 /label=BBa_B0032 misc_feature 83..1666 /label=BBa_K1390004 RBS 1675..1687 /label=RBS-3\Weak RBS 1681..1684 /label=RBS misc_feature 1675..1687 /label=BBa_B0032 RBS 1675..1687 /label=BBa_B0032 misc_feature 1694..2863 /label=BBa_K1390005 RBS 2872..2884 /label=RBS-3\Weak RBS 2878..2881 /label=RBS misc_feature 2872..2884 /label=BBa_B0032 RBS 2872..2884 /label=BBa_B0032 misc_feature 2891..3316 /label=BBa_K1390001 RBS 3325..3337 /label=RBS-3\Weak RBS 3331..3334 /label=RBS misc_feature 3325..3337 /label=BBa_B0032 RBS 3325..3337 /label=BBa_B0032 misc_feature 3344..3853 /label=BBa_K1390006 RBS 3862..3874 /label=RBS-3\Weak RBS 3868..3871 /label=RBS misc_feature 3862..3874 /label=BBa_B0032 RBS 3862..3874 /label=BBa_B0032 misc_feature 3881..4192 /label=BBa_K1390003 RBS 4201..4213 /label=RBS-3\Weak RBS 4207..4210 /label=RBS misc_feature 4201..4213 /label=BBa_B0032 RBS 4201..4213 /label=BBa_B0032 misc_feature 4220..5266 /label=BBa_K1390002 stem_loop 5286..5329 /label=stem_loop misc_feature 5275..5354 /label=BBa_B0010 terminator 5275..5354 /label=BBa_B0010 stem_loop 5370..5389 /label=T7 TE misc_feature 5396..5396 /label=stop polyA_site 5390..5403 /label=polya misc_feature 5363..5403 /label=BBa_B0012 terminator 5363..5403 /label=BBa_B0012 terminator 5275..5403 /label=BBa_B0015 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagtcacaca ggaaagtact agatggcact tagcaccgca accaaggccg cgacggacgc 121 gctggctgcc aatcgggcac ccaccagcgt gaatgcacag gaagtgcacc gttggctcca 181 gagcttcaac tgggatttca agaacaaccg gaccaagtac gccaccaagt acaagatggc 241 gaacgagacc aaggaacagt tcaagctgat cgccaaggaa tatgcgcgca tggaggcagt 301 caaggacgaa aggcagttcg gtagcctgca agatgcgctg acccgcctca acgccggtgt 361 tcgcgttcat ccgaagtgga acgagaccat gaaagtggtt tcgaacttcc tggaagtggg 421 cgaatacaac gccatcgccg ctaccgggat gctgtgggat tccgcccagg cggcggaaca 481 gaagaacggc tatctggccc aggtgttgga tgaaatccgc cacacccacc agtgtgccta 541 cgtcaactac tacttcgcga agaacggcca ggacccggcc ggtcacaacg atgctcgccg 601 cacccgtacc atcggtccgc tgtggaaggg catgaagcgc gtgttttccg acggcttcat 661 ttccggcgac gccgtggaat gctccctcaa cctgcaactg gtgggtgagg cctgcttcac 721 caatccgctg atcgtcgcag tgaccgaatg ggctgccgcc aacggcgatg aaatcacccc 781 gacggtgttc ctgtcgatcg agaccgacga actgcgccac atggccaacg gttaccagac 841 cgtcgtttcc atcgccaacg atccggcttc cgccaagtat ctcaacacgg acctgaacaa 901 cgccttctgg acccagcaga agtacttcac gccggtgttg ggcatgctgt tcgagtatgg 961 ctccaagttc aaggtcgagc cgtgggtcaa gacgtggaac cgctgggtgt acgaggactg 1021 gggcggcatc tggatcggcc gtctgggcaa gtacggggtg gagtcgccgc gcagcctcaa 1081 ggacgccaag caggacgctt actgggctca ccacgacctg tatctgctgg cttatgcgct 1141 gtggccgacc ggcttcttcc gtctggcgct gccggatcag gaagaaatgg agtggttcga 1201 ggccaactac cccggctggt acgaccacta cggcaagatc tacgaggaat ggcgcgcccg 1261 cggttgcgag gatccgtcct cgggcttcat cccgctgatg tggttcatcg aaaacaacca 1321 tcccatctac atcgatcgcg tgtcgcaagt gccgttctgc ccgagcttgg ccaagggcgc 1381 cagcaccctg cgcgtgcacg agtacaacgg ccagatgcac accttcagcg accagtgggg 1441 cgagcgcatg tggctggccg agccggagcg ctacgagtgc cagaacatct tcgaacagta 1501 cgaaggacgc gaactgtcgg aagtgatcgc cgaactgcac gggctgcgca gtgatggcaa 1561 gaccctgatc gcccagccgc atgtccgtgg cgacaagctg tggacgttgg acgatatcaa 1621 acgcctgaac tgcgtcttca agaacccggt gaaggcattc aattgatact agagtcacac 1681 aggaaagtac tagatgagca tgttaggaga aagacgccgc ggtctgaccg atccggaaat 1741 ggcggccgtc attttgaagg cgcttcctga agctccgctg gacggcaaca acaagatggg 1801 ttatttcgtc accccccgct ggaaacgctt gacggaatat gaagccctga ccgtttatgc 1861 gcagcccaac gccgactgga tcgccggcgg cctggactgg ggcgactgga cccagaaatt 1921 ccacggcggc cgcccttcct ggggcaacga gaccacggag ctgcgcaccg tcgactggtt 1981 caagcaccgt gacccgctcc gccgttggca tgcgccgtac gtcaaggaca aggccgagga 2041 atggcgctac accgaccgct tcctgcaagg ttactccgcc gacggtcaga tccgggcgat 2101 gaacccgacc tggcgggacg agttcatcaa ccggtattgg ggcgccttcc tgttcaacga 2161 atacggattg ttcaacgctc attcgcaggg cgcccgggag gcgctgtcgg acgtaacccg 2221 cgtcagcctg gctttctggg gcttcgacaa gatcgacatc gcccagatga tccaactcga 2281 acggggtttc ctcgccaaga tcgtacccgg tttcgacgag tccacagcgg tgccgaaggc 2341 cgaatggacg aacggggagg tctacaagag cgcccgtctg gccgtggaag ggctgtggca 2401 ggaggtgttc gactggaacg agagcgcttt ctcggtgcac gccgtctatg acgcgctgtt 2461 cggtcagttc gtccgccgcg agttctttca gcggctggct ccccgcttcg gcgacaatct 2521 gacgccattc ttcatcaacc aggcccagac atacttccag atcgccaagc agggcgtaca 2581 ggatctgtat tacaactgtc tgggtgacga tccggagttc agcgattaca accgtaccgt 2641 gatgcgcaac tggaccggca agtggctgga gcccacgatc gccgctctgc gcgacttcat 2701 ggggctgttt gcgaagctgc cggcgggcac cactgacaag gaagaaatca ccgcgtccct 2761 gtaccgggtg gtcgacgact ggatcgagga ctacgccagc aggatcgact tcaaggcgga 2821 ccgcgatcag atcgttaaag cggttctggc aggattgaaa tgatactaga gtcacacagg 2881 aaagtactag atgagcgtaa acagcaacgc atacgacgcc ggcatcatgg gcctgaaagg 2941 caaggacttc gccgatcagt tctttgccga cgaaaaccaa gtggtccatg aaagcgacac 3001 ggtcgttctg gtcctcaaga agtcggacga gatcaatacc tttatcgagg agatccttct 3061 gacggactac aagaagaacg tcaatccgac ggtaaacgtg gaagaccgcg cgggttactg 3121 gtggatcaag gccaacggca agatcgaggt cgattgcgac gagatttccg agctgttggg 3181 gcggcagttc aacgtctacg acttcctcgt cgacgtttcc tccaccatcg gccgggccta 3241 taccctgggc aacaagttca ccattaccag tgagctgatg ggcctggacc gcaagctcga 3301 agactatcac gcttaatact agagtcacac aggaaagtac tagatggcga aactgggtat 3361 acacagcaac gacacccgcg acgcctgggt gaacaagatc gcgcagctca acaccctgga 3421 aaaagcggcc gagatgctga agcagttccg gatggaccac accacgccgt tccgcaacag 3481 ctacgaactg gacaacgact acctctggat cgaggccaag ctcgaagaga aggtcgccgt 3541 cctcaaggca cgcgccttca acgaggtgga cttccgtcat aagaccgctt tcggcgagga 3601 tgccaagtcc gttctggacg gcaccgtcgc gaagatgaac gcggccaagg acaagtggga 3661 ggcggagaag atccatatcg gtttccgcca ggcctacaag ccgccgatca tgccggtgaa 3721 ctatttcctg gacggcgagc gtcagttggg gacccggctg atggaactgc gcaacctcaa 3781 ctactacgac acgccgctgg aagaactgcg caaacagcgc ggtgtgcggg tggtgcatct 3841 gcaatcgccg tgatactaga gtcacacagg aaagtactag atggtcgaat cggcatttca 3901 gccattttcg ggcgacgcag acgaatggtt cgaggaacca cggccccagg ccggtttctt 3961 cccttccgcg gactggcatc tgctcaaacg ggacgagacc tacgcagcct atgccaagga 4021 tctcgatttc atgtggcggt gggtcatcgt ccgggaagaa aggatcgtcc aggagggttg 4081 ctcgatcagc ctggagtcgt cgatccgcgc cgtgacgcac gtactgaatt attttggtat 4141 gaccgaacaa cgcgccccgg cagaggaccg gaccggcgga gttcaacatt gatactagag 4201 tcacacagga aagtactaga tgcagcgagt tcacactatc acggcggtga cggaggatgg 4261 cgaatcgctc cgcttcgaat gccgttcgga cgaggacgtc atcaccgccg ccctgcgcca 4321 gaacatcttt ctgatgtcgt cctgccggga gggcggctgt gcgacctgca aggccttgtg 4381 cagcgaaggg gactacgacc tcaagggctg tagcgttcag gcgctgccgc cggaagagga 4441 ggaggaaggg ttggtgttgt tgtgccggac ctacccgaag accgacctgg aaatcgaact 4501 gccctatacc cattgccgca tcagttttgg tgaggtcggc agtttcgagg cggaggtcgt 4561 cggcctcaac tgggtttcga gcaacaccgt ccagtttctt ttgcagaagc ggcccgacga 4621 gtgcggcaac cgtggcgtga aattcgaacc cggtcagttc atggacctga ccatccccgg 4681 caccgatgtc tcccgctcct actcgccggc gaaccttcct aatcccgaag gccgcctgga 4741 gttcctgatc cgcgtgttac cggagggacg gttttcggac tacctgcgca atgacgcgcg 4801 tgtcggacag gtcctctcgg tcaaagggcc actgggcgtg ttcggtctca aggagcgggg 4861 catggcgccg cgctatttcg tggccggcgg caccgggttg gcgccggtgg tctcgatggt 4921 gcggcagatg caggagtgga ccgcgccgaa cgagacccgc atctatttcg gtgtgaacac 4981 cgagccggaa ttgttctaca tcgacgagct caaatccctg gaacgatcga tgcgcaatct 5041 caccgtgaag gcctgtgtct ggcacccgag cggggactgg gaaggcgagc agggctcgcc 5101 catcgatgcg ttgcgggaag acctggagtc ctccgacgcc aacccggaca tttatttgtg 5161 cggtccgccg ggcatgatcg atgccgcctg cgagctggta cgcagccgcg gtatccccgg 5221 cgaacaggtc ttcttcgaaa aattcctgcc gtccggggcg gcctgatact agagccaggc 5281 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 5341 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 5401 ata //