BBa_K1391000 1 BBa_K1391000 Gantenerumab Variable Human Ig-M Heavy Chain 2014-10-09T11:00:00Z 2015-05-08T01:10:14Z Fusion protein of 3 human peptide sequences This is a fusion of a beta-amyloid specific variable region on a human IgM heavy chain with a membrane localization tag that causes it to be sent to the ER and eventually the membrane with other parts. The heavy and light chain stick to and extracellular antigen (beta-amyloid) and cause the B-Cell Receptor (BCR) complex to dimerize. The IgM Heavy chain, IgM light chain, CD79A subunit, and CD79B subunit require processing in the ER and must all be expressed in order to membrane localize instead of being retained in the ER by quality checking mechanisms. Binding to the antigen (beta-amyloid) causes two BCR complexes to dimerize. This allows Lyn to phosphorylate the CD79 heterodimer which causes the recruitment of Syk. As a warning, recruited Syk can cause cross talk with native cell signaling pathways as can other proteins. Care must be taken to check protein interactions and point mutate Syk if needed. We attach a protease (TEV protease) to Syk and a transcriptional activator (Gal4-VP16) to CD79 using a cleavage site (TEV cleavage site). When The BCR is activated by our antigen, Syk-TEVp cleaves the cleavage sit and releases Gal4-VP16 which can activate a synthetic system. The variable regions of the heavy and light chains can be switched for the variable regions of another monoclonal antibody with known cDNA or peptide sequence to change which antigen causes activation. It is important to make sure an appropriate localization tag is attached to the new heavy and light chains. The tag must be cleaved correctly, leaving no residue and removing no variable region peptides. false false _1768_ 0 22906 9 It's complicated false Gantenerumab has shown therapeutic potential in recent clinical trials; Human Ig-M can be anchored to the membrane to interact with the b-cell receptor (BCR) complex; Human antibody membrane localization tag is natively found in human, membrane-bound antibodies false Alexa Garcia BBa_K1391000_sequence 1 atggagttcgggctgtcctggctgtttctggtcgcgatcctgaaaggcgtccagtgccaagtggagctcgtggaatctggcgggggtcttgtgcagcccggcggctcacttcgactgagttgtgcagctagtggctttaccttctcatcttatgctatgagctgggtacggcaggccccaggaaaaggtttggagtgggtgtccgccatcaacgccagcggtacccgcacttattacgcggattctgtgaagggccgctttaccatctcacgggacaacagtaagaacactctctacctccagatgaattcattgcgcgctgaggacacggcagtttattattgtgcgcgcggaaagggtaatacccacaaaccttatggctatgtgcgctactttgacgtgtggggacagggcaccctggtgactgtatctagtggctctgcctccgccccaacactgtttcctctggttagctgtgagaattccccttccgatacatcttctgtcgcagtcgggtgcttggcacaggacttcctgccagactcaataacgcttagttggaagtataagaataatagcgatatttcctcaacacgcggatttccgtctgtactgcgggggggaaagtacgcggcaaccagccaagtactcctcccatctaaggatgttatgcagggaacagacgaacatgtcgtatgtaaggttcagcatcccaacggcaacaaggagaaaaacgttcctttgcccgtgatcgccgagctgccccccaaagtgtcagtgttcgtgccgccacgagacggctttttcgggaatccaagaaaatccaagctcatctgccaggccactggattttctccaaggcagatccaggtgagctggctcagagaggggaagcaggttggctccggggtgacgactgaccaggtccaggcagaggctaaggaaagtggacctacgacatacaaggtgacatctacgctcactattaaagagagcgactggctgggacaaagtatgtttacctgtcgggtggatcacaggggtcttacatttcaacagaacgctagtagtatgtgcgtgcctgaccaggatacagcaataagggtttttgccattcccccttcatttgcatccattttcctgaccaagtcaactaaactgacttgtctggttacagatctgacaacatacgattctgtcacgatttcatggacccggcagaacggggaggccgtgaagacccatacaaatataagcgaaagtcaccccaatgccaccttctcagccgtgggagaggcttccatctgtgaggatgactggaactccggcgagcgattcacttgcacagtgacacacaccgacctcccgtcacccttgaaacaaaccattagtagacctaaaggcgttgctctgcatcgacccgatgtttaccttcttccacccgcacgcgagcagctgaatctcagggagtcagccaccattacgtgtcttgtaacaggattttctccagctgacgtgttcgtgcaatggatgcaacggggccagcccctgagtccagagaaatatgtcacaagcgctccaatgcctgaaccacaagccccgggaagatactttgcacactcaattctgaccgtctctgaggaagaatggaacacgggtgagacatacacgtgtgtggcacacgaagctctgcccaacagagtaacggagcggactgttgataaaagcaccgagggcgaggtgtcagctgacgaggaggggtttgagaacctgtgggcaacagcaagcacatttattgttctctttttgctttccctgttctacagcacgaccgtgaccctctttaaggtgaagtagtag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z