LOCUS BBa_K1391113 10740 bp DNA linear 22-Nov-2024 DEFINITION pEXPR hEF1a:PirB_TCS_Gal4VP16 ACCESSION BBa_K1391113 VERSION BBa_K1391113.1 FEATURES Location/Qualifiers misc_feature 5515..8037 /label=BBa_K1391013 ORIGIN 1 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 61 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 121 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 181 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 241 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 301 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 361 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 421 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 481 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 541 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 601 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 661 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 721 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 781 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 841 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 901 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 961 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 1021 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 1081 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 1141 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 1201 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 1261 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 1321 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 1381 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 1441 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 1501 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 1561 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 1621 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 1681 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 1741 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 1801 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 1861 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 1921 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa 1981 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc 2041 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga 2101 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag 2161 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt 2221 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta 2281 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag 2341 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg 2401 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg 2461 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg 2521 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 2581 ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtacctgc tttctctgac 2641 cagcattctc tcccctgggc ctgtgccgct ttctgtctgc agcttgtggc ctgggtcacc 2701 tctacggctg gcccagatcc ttccctgccg cctccttcag gttccgtctt cctccactcc 2761 ctcttcccct tgctctctgc tgtgttgctg cccaaggatg ctctttccgg agcacttcct 2821 tctcggcgct gcaccacgtg atgtcctctg agcggatcct ccccgtgtct gggtcctctc 2881 cgggcatctc tcctccctca cccaacccca tgccgtcttc actcgctggg ttcccttttc 2941 cttctccttc tggggcctgt gccatctctc gtttcttagg atggccttct ccgacggatg 3001 tctcccttgc gtcccgcctc cccttcttgt aggcctgcat catcaccgtt tttctggaca 3061 accccaaagt accccgtctc cctggcttta gccacctctc catcctcttg ctttctttgc 3121 ctggacaccc cgttctcctg tggattcggg tcacctctca ctcctttcat ttgggcagct 3181 cccctacccc ccttacctct ctagtctgtg ctagctcttc cagccccctg tcatggcatc 3241 ttccaggggt ccgagagctc agctagtctt cttcctccaa cccgggcccc tatgtccact 3301 tcaggacagc atgtttgctg cctccaggga tcctgtgtcc ccgagctggg accaccttat 3361 attcccaggg ccggttaatg tggctctggt tctgggtact tttatctgtc ccctccaccc 3421 cacagtgggg caagcttatt accctgttat ccctaaccgg tttaccgagc tcttattggt 3481 tttcaaactt cattgactgt gccaagcttc tgacctcttc tcttcctccc acagggcctc 3541 gagacaattg catcgatggt accagtgaaa gtctgcaatg aattcgagtt ggcgcgcctg 3601 tcattctaaa tctctctttc agcctaaagc tttttccccg tatcccccca ggtgtctgca 3661 ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc 3721 ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg 3781 agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg 3841 gctttgggag ggggctgtcc ccgtgagctc ccaggcgcgc ctgtcattct aaatctctct 3901 ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga 3961 gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac 4021 gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc 4081 tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg 4141 tccccgtgag ctcccaggcg cgccaactcg gtaccgtatc gatgtcgacg ttaacgctag 4201 tgatatcaac tttgtataga aaagttggct ccgaattcgc ccttattaat tggctccggt 4261 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttgtg gggaggggtc 4321 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 4381 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 4441 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt 4501 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc 4561 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg 4621 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc 4681 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag 4741 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt 4801 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc 4861 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca 4921 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg 4981 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga 5041 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc 5101 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc 5161 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg 5221 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc 5281 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg 5341 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa 5401 agtttttttc ttccatttca ggtggatccc aagggcgaat tcgacccaag tttgtacaaa 5461 aaagcaggct ggatcccggt accctcgaca cctgctgaca ggtacgccgc caccatgtcc 5521 tgcaccttca cagccctgct ccgtcttgga ctgactctga gcctctggat cccagtgctg 5581 acagggtccc tccctaagcc tatcctcaga gtacagccag actctgtggt gtccaggagg 5641 actaaggtga ccttcttgtg tgaagagaca attggagcca atgagtaccg cctctataaa 5701 gatggaaagc tatataaaac cgtaacaaag aacaaacaga agccagaaaa caaggctgaa 5761 ttctcattct caaatgtaga cctgagtaat gcaggtcaat atcgatgttc ctacagcacc 5821 cagtataaat catcaggcta cagtgacctc ctggagctgg tggtgacagg acactactgg 5881 acacccagcc ttttagccca agccagccct gtggtaactt caggagggta tgtcaccctc 5941 cagtgtgagt cctggcacaa cgatcacaag ttcattctga ctgtagaagg accacagaag 6001 ctctcgtgga cacaagactc acagtataat tactctacaa ggaagtacca cgccctgttc 6061 tctgtgggcc ctgtgacccc caaccagaga tggatatgca gatgttacag ttatgacagg 6121 aacagaccat atgtgtggtc acctccaagt gaatccgtgg agctcctggt gtcaggtaat 6181 ctccaaaaac caaccatcaa ggctgaacca ggatctgtga tcacctccaa aagagcaatg 6241 accatctggt gtcaggggaa cctggatgca gaagtatatt ttctgcataa tgagaaaagc 6301 caaaaaacac agagcacaca gaccctacag gagcctggga acaagggcaa gttcttcatc 6361 ccttctgtga cactacaaca tgcagggcaa tatcgctgtt attgttacgg ctcagctggt 6421 tggtcacagc ccagtgacac cctggagctg gtggtgacag gaatctatga atactatgaa 6481 cccaggctgt cagtactgcc cagccctgtg gtgacagctg gagggaacat gacactccac 6541 tgtgcctcag actttcccta cgataaattc attctcacca aggaagataa gaaattcggc 6601 aactcactgg acacagagca tatatcttct agtggacagt accgagccct gtttattata 6661 ggacccacaa ccccaaccca tacaggggca ttcagatgtt acggttacta caagaatgcc 6721 ccacagctgt ggtcagtacc tagtgctctc caacaaatac tcatctcagg gctgtccaag 6781 aagccctctc tgctgactca ccaaggccat atcctggacc ctggaatgac cctcaccctg 6841 cagtgtttct ctgacatcaa ctatgacaga tttgctctgc acaaggtggg gggagctgac 6901 atcatgcagc actctagcca gcagactgac actggcttct ctgtggccaa cttcacactg 6961 ggctatgtga gtagctccac tggaggccaa tacagatgct atggtgcaca caacctctcc 7021 tctgagtggt cagcctccag tgagcccctg gacatcctga tcacaggaca gctccctctc 7081 actccttccc tctcagtgca gcctaaccac acagtgcact caggagaaac cgtgagcctg 7141 ctgtgttggt caatggactc tgtggatact ttcattctgt ccaaggaggg atcagcccag 7201 caacccctgc gactaaaatc aaagtcccat gatcagcagt cccaggcaga attctccatg 7261 agtgctgtga cctcccatct ctcaggcacc tacaggtgct atggagctca agactcatct 7321 ttctacctct tgtcatctgc cagtgcccct gtggagctca cagtctcagg acccatcgaa 7381 acctctaccc cgccacccac aatgtccatg ccactaggtg gactgcatat gtacctgaag 7441 gctctcattg gagtgtctgt ggccttcatc ctgttcctct tcatcttcat cttcattctt 7501 cttcgacgaa gacatcgggg aaaattcagg aaagatgtcc agaaagagaa agacttgcaa 7561 ctttcttcag gagctgaaga gcccataacc aggaaaggag aactccagaa gaggcccaac 7621 ccagctgctg ccacccagga agaaagccta tatgcttcag tggaggacat gcaaactgag 7681 gatggagtgg agctgaacag ctggacacca cctgaggaag atccccaggg agagacttat 7741 gcccaggtga aaccctccag gctcaggaag gcaggacatg tctcaccttc tgtcatgtca 7801 agggaacaac tgaacacaga atatgaacaa gcagaagagg gccaaggagc aaacaatcag 7861 gctgccgaat ctggggagtc ccaggatgtg acctatgccc agctgtgcag caggacactc 7921 agacaggggg cagctgcatc tcctctctcc caggcagggg aagccccaga ggagcccagt 7981 gtatatgcta ctctggcggc tgctcgtcca gaggctgttc ccaaggacat ggagcaagag 8041 aacctgtact tccagctgat ggcccccccg accgatgtca gcctggggga cgagctccac 8101 ttagacggcg aggacgtggc gatggcgcat gccgacgcgc tagacgattt cgatctggac 8161 atgttggggg acggggattc cccgggtccg ggatttaccc cccacgactc cgccccctac 8221 ggcgctctgg atatggccga cttcgagttt gagcagatgt ttaccgatgc ccttggaatt 8281 gacgagtacg gtgggacgcg tatgaagcta ctgtcttcta tcgaacaagc atgcgatatt 8341 tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag 8401 aacaactggg agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat 8461 ctgacagaag tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct 8521 cgagaagacc ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta 8581 acaggattat ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca 8641 gtggagactg atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg 8701 gaagagagta gtaacaaagg tcaaagacag ttgactgtat aaaagcttgc ttcagtgcag 8761 gtgaattcta ccacccagct ttcttgtaca aagtggtacg cgtgaattca ctcctcaggt 8821 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca 8881 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct 8941 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg 9001 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt 9061 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata 9121 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc 9181 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc 9241 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc 9301 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt 9361 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 9421 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 9481 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcg 9541 acagtactaa gcttggtgcg tttttatgct tgtagtattg tataatgttt ttaagatcct 9601 taattaatag ggataacagg gtaatctcga ggtttaaacg tcgacgcggc cgctactagg 9661 gacaggattg gtgacagaaa agccccatcc ttaggcctcc tccttcctag tctcctgata 9721 ttgggtctaa cccccacctc ctgttaggca gattccttat ctggtgacac acccccattt 9781 cctggagcca tctctctcct tgccagaacc tctaaggttt gcttacgatg gagccagaga 9841 ggatcctggg agggagagct tggcaggggg tgggagggaa gggggggatg cgtgacctgc 9901 ccggttctca gtggccaccc tgcgctaccc tctcccagaa cctgagctgc tctgacgcgg 9961 ctgtctggtg cgtttcactg atcctggtgc tgcagcttcc ttacacttcc caagaggaga 10021 agcagtttgg aaaaacaaaa tcagaataag ttggtcctga gttctaactt tggctcttca 10081 cctttctagt ccccaattta tattgttcct ccgtgcgtca gttttacctg tgagataagg 10141 ccagtagcca gccccgtcct ggcagggctg tggtgaggag gggggtgtcc gtgtggaaaa 10201 ctccctttgt gagaatggtg cgtcctaggt gttcaccagg tcgtggccgc ctctactccc 10261 tttctctttc tccatccttc tttccttaaa gagtccccag tgctatctgg gacatattcc 10321 tccgcccaga gcagggtccc gcttccctaa ggccctgctc tgggcttctg ggtttgagtc 10381 cttggcaagc ccaggagagg cgctcaggct tccctgtccc ccttcctcgt ccaccatctc 10441 atgcccctgg ctctcctgcc ccttccctac aggggttcct ggctctgctc tccaccgcgg 10501 tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg 10561 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 10621 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 10681 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc //