BBa_B1006
1
BBa_B1006
Terminator (artificial, large, %T~>90)
2006-08-30T11:00:00Z
2015-08-31T04:07:21Z
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
Released HQ 2013
Artificial terminator, estimated %T~>90%
*8bp stem, 6nt loop
*Bidirectional, estimated reverse %T~>90%
false
true
_41_
0
745
41
In stock
false
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
true
Haiyao Huang
annotation1898429
1
modified thr terminator
range1898429
1
10
31
annotation1898431
1
PolyA
range1898431
1
1
9
annotation1898430
1
PolyA
range1898430
1
32
39
annotation1898428
1
B1006
range1898428
1
1
39
BBa_K124014
1
BBa_K124014
Bacteriophage Holin Gene pS105
2008-10-08T11:00:00Z
2015-05-08T01:09:44Z
This part was graciously donated to the Brown iGEM Team by Ry Young at Texas A&M.
The Cell Lysis Cassette consists of an S, R, and Rz protein. When paired with an activator, the cassette is expressed and cell lysis is induced. The S105 strain of the cassette has the initial 6 base pairs at the beginning of the wild type sequence removed. This causes cell lysis to be induced at a faster rate than the wild type.
false
false
_221_
0
3367
9
It's complicated
true
No design considerations.
true
Katherine Jacobs
BBa_K1392971
1
BBa_K1392971
Holin+Terminator
2014-10-09T11:00:00Z
2015-05-08T01:10:15Z
Brown iGEM Team by Ry Young at Texas A&M
Modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
This part comes from a Bacteriophage Holin Gene pS105
This is a variation of the S gene of the "Cell Lysis Cassette." When it is paired with an activator, the cassette is expressed and cell lysis is induced.
The S105 gene has the initial 6 base pairs at the beginning of the wild type S gene sequence removed. This causes cell lysis to be induced at a faster rate than the wild type.
The added Terminator is artificial and large.
false
false
_1769_
0
16902
9
It's complicated
false
No design considerations during Holin Gene
Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 9 residues.
false
Şeniz Y??ksel İlkem Kumru
component2415817
1
BBa_B1006
component2415812
1
BBa_K124014
annotation2415817
1
BBa_B1006
range2415817
1
326
364
annotation2415812
1
BBa_K124014
range2415812
1
1
317
BBa_K124014_sequence
1
gctgctaaaaaagccggagtagaagatggtagaaatcaataagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttctataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagtatgccagaaaaacatgacctgttggccgccattctcgcggcaaaggaacaaggcatcggggcaatccttgcgtttgcaatggcgtaccttcgcggcaga
BBa_B1006_sequence
1
aaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_K1392971_sequence
1
gctgctaaaaaagccggagtagaagatggtagaaatcaataagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttctataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagtatgccagaaaaacatgacctgttggccgccattctcgcggcaaaggaacaaggcatcggggcaatccttgcgtttgcaatggcgtaccttcgcggcagatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z