LOCUS       BBa_K1392972            2551 bp    DNA     linear       22-Nov-2024
DEFINITION  Composite part fot TetLVA TetPromoter and T4
ACCESSION   BBa_K1392972
VERSION     BBa_K1392972.1  
FEATURES             Location/Qualifiers
     CDS             1..879
                     /label=AraC
     misc_feature    909..924
                     /label=AraO
     misc_feature    1066..1077
                     /label=AraO1
     promoter        1153..1181
                     /label=AraPromoter
     promoter        1..1209
                     /label=BBa_K808000
     misc_feature    1222..1225
                     /label=conserved
     RBS             1218..1229
                     /label=BBa_B0034
     CDS             1239..1855
                     /label=tetR
     misc_feature    1856..1889
                     /label=SsrA
     misc_feature    1896..1920
                     /label=Help:Barcodes
     CDS             1236..1920
                     /label=BBa_C0040
     misc_feature    1929..1967
                     /label=B1006
     stem_loop       1938..1959
                     /label=modified thr terminator
     polyA_site      1960..1967
                     /label=PolyA
     polyA_site      1929..1937
                     /label=PolyA
     terminator      1929..1967
                     /label=BBa_B1006
     misc_feature    1976..1994
                     /label=TetR 1
     misc_feature    1976..2029
                     /label=BBa_R0040
     promoter        1995..2000
                     /label=-35
     misc_feature    2001..2019
                     /label=TetR 2
     promoter        2018..2023
                     /label=-10
     promoter        1976..2029
                     /label=BBa_R0040
     CDS             2054..2548
                     /label=T4 Endolysin
     CDS             2038..2551
                     /label=BBa_K112806
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 tgggctaact actagagaaa gaggagaaat actagatgtc cagattagat aaaagtaaag
     1261 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta
     1321 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc
     1381 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc
     1441 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg
     1501 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac
     1561 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga
     1621 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc
     1681 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat
     1741 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg
     1801 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgctg
     1861 caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac
     1921 tactagagaa aaaaaaaccc cgcccctgac agggcggggt ttttttttac tagagtccct
     1981 atcagtgata gagattgaca tccctatcag tgatagagat actgagcact actagagata
     2041 cttaggaggt attatgaata tatttgaaat gttacgtata gatgaaggtc ttagacttaa
     2101 aatctataaa gacacagaag gctattacac tattggcatc ggtcatttgc ttacaaaaag
     2161 tccatcactt aatgctgcta aatctgaatt agataaagct attgggcgta attgcaatgg
     2221 tgtaattaca aaagatgagg ctgaaaaact ctttaatcag gatgttgatg ctgctgttcg
     2281 cggaatcctg agaaatgcta aattaaaacc ggtttatgat tctcttgatg cggttcgtcg
     2341 ctgtgcattg attaatatgg ttttccaaat gggagaaacc ggtgtggcag gatttactaa
     2401 ctctttacgt atgcttcaac aaaaacgctg ggatgaagca gcagttaact tagctaaaag
     2461 tagatggtat aatcaaacac ctaatcgcgc aaaacgagtc attacaacgt ttagaactgg
     2521 cacttgggac gcgtataaaa atctataaag c
//