LOCUS       BBa_K1392973            3243 bp    DNA     linear       18-May-2024
DEFINITION  AracC+Antiholin+RBS+TetLVA+Term+TetR Promoter+ T4 
            Endolysin+Holin+Terminator
ACCESSION   BBa_K1392973
VERSION     BBa_K1392973.1  
FEATURES             Location/Qualifiers
     CDS             1..879
                     /label=AraC
     misc_feature    909..924
                     /label=AraO
     misc_feature    1066..1077
                     /label=AraO1
     promoter        1153..1181
                     /label=AraPromoter
     promoter        1..1209
                     /label=BBa_K808000
     misc_feature    1218..1232
                     /label=insulator
     RBS             1233..1252
                     /label=rbs
     CDS             1253..1549
                     /label=antiholin
     misc_feature    1218..1549
                     /label=BBa_K515004
     CDS             1559..2175
                     /label=tetR
     misc_feature    2176..2209
                     /label=SsrA
     misc_feature    2216..2240
                     /label=Help:Barcodes
     CDS             1556..2240
                     /label=BBa_C0040
     misc_feature    2249..2287
                     /label=B1006
     stem_loop       2258..2279
                     /label=modified thr terminator
     polyA_site      2280..2287
                     /label=PolyA
     polyA_site      2249..2257
                     /label=PolyA
     terminator      2249..2287
                     /label=BBa_B1006
     misc_feature    2296..2314
                     /label=TetR 1
     misc_feature    2296..2349
                     /label=BBa_R0040
     promoter        2315..2320
                     /label=-35
     misc_feature    2321..2339
                     /label=TetR 2
     promoter        2338..2343
                     /label=-10
     promoter        2296..2349
                     /label=BBa_R0040
     CDS             2374..2868
                     /label=T4 Endolysin
     CDS             2358..2871
                     /label=BBa_K112806
     CDS             2880..3196
                     /label=BBa_K124014
     misc_feature    3205..3243
                     /label=B1006
     stem_loop       3214..3235
                     /label=modified thr terminator
     polyA_site      3236..3243
                     /label=PolyA
     polyA_site      3205..3213
                     /label=PolyA
     terminator      3205..3243
                     /label=BBa_B1006
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 tgggctaact actagagcag cctgcggtcc gggcgataat aaggaggtac taatggcctt
     1261 aaaagcaaca gcactttttg ccatgctagg attgtcattt gttttatctc catcgattga
     1321 agcgaatgtc gatcctcatt ttgataaatt tatggaatct ggtattaggc acgtttatat
     1381 gctttttgaa aataaaagcg tagaatcgtc tgaacaattc tatagtttta tgagaacgac
     1441 ctataaaaat gacccgtgct cttctgattt tgaatgtata gagcgaggcg cggagatggc
     1501 acaatcatac gctagaatta tgaacattaa attggagact gaataataat actagatgtc
     1561 cagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat
     1621 cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta
     1681 ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag
     1741 gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa
     1801 cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg
     1861 tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca
     1921 acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt
     1981 aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac
     2041 tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc
     2101 agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa
     2161 atgtgaaagt gggtccgctg caaacgacga aaactacgct ttagtagctt aataacactg
     2221 atagtgctag tgtagatcac tactagagaa aaaaaaaccc cgcccctgac agggcggggt
     2281 ttttttttac tagagtccct atcagtgata gagattgaca tccctatcag tgatagagat
     2341 actgagcact actagagata cttaggaggt attatgaata tatttgaaat gttacgtata
     2401 gatgaaggtc ttagacttaa aatctataaa gacacagaag gctattacac tattggcatc
     2461 ggtcatttgc ttacaaaaag tccatcactt aatgctgcta aatctgaatt agataaagct
     2521 attgggcgta attgcaatgg tgtaattaca aaagatgagg ctgaaaaact ctttaatcag
     2581 gatgttgatg ctgctgttcg cggaatcctg agaaatgcta aattaaaacc ggtttatgat
     2641 tctcttgatg cggttcgtcg ctgtgcattg attaatatgg ttttccaaat gggagaaacc
     2701 ggtgtggcag gatttactaa ctctttacgt atgcttcaac aaaaacgctg ggatgaagca
     2761 gcagttaact tagctaaaag tagatggtat aatcaaacac ctaatcgcgc aaaacgagtc
     2821 attacaacgt ttagaactgg cacttgggac gcgtataaaa atctataaag ctactagagg
     2881 ctgctaaaaa agccggagta gaagatggta gaaatcaata agcaatctcg cttatataac
     2941 gagcgtgttt atcggctaca tcggtactga ctcgattggt tcgcttatca aacgcttcta
     3001 taatggcggt gcgtttacaa aaacagtaat cgacgcaacg atgtgcgcca ttatcgcctg
     3061 gttcattcgt gaccttctcg acttcgccgg actaagtatg ccagaaaaac atgacctgtt
     3121 ggccgccatt ctcgcggcaa aggaacaagg catcggggca atccttgcgt ttgcaatggc
     3181 gtaccttcgc ggcagatact agagaaaaaa aaaccccgcc cctgacaggg cggggttttt
     3241 ttt
//