BBa_K1393002 1 BBa_K1393002 cyn operon in Escherichia coli BL21(DE3) (cynR+cynT+cynS) 2014-10-01T11:00:00Z 2015-05-08T01:10:15Z It come from the genome of E.coli. We constructed and improved standard BioBrick Parts about cyanide detoxification.This part conteins CynR,CynT,CynS three genes.The CynT and CynS belong to cynTSX operon. The transcriptional activation of the cynTSX polycistronic message is under the positive control of the CynR protein and cyanide. The highlight is that the cynTSX operon enables Escherichia coli K-12 to degrade and use cyanide as a sole nitrogen source. And the most important point is the promoter of this operon is positively regulated by cyanide and the CynR protein, which means this part can play a role only in the exist of cyanide. false false _1770_ 0 20256 9 It's complicated false The CynR gene is located upstream of the cynTSX operon and is transcribed in the opposite direction of the cyn operon. CynR negatively regulates its own synthesis independently of cyanide. What???s more,the cynR promoter was found to overlap the cynTSX promoter on the opposite strand. Moreover furthermore,cyanate degradation requires carbonic anhydrase encoded by cynT and cyanide hydrolase encoded by cynS.CynS catalyzes the bicarbonate-dependent hydrolysis of cyanide into ammonia and two molecules of carbon dioxide. CynT recycles the carbon dioxide into bicarbonate.The third gene of the cyn operon, cynX, encodes a hydrophobic protein of undetermined function.So we didn???t choose it. false Jiajun Tan annotation2392296 1 CynT_RBS range2392296 1 1168 1171 annotation2392294 1 CynS range2392294 1 4 471 annotation2392298 1 CynS_RBS range2392298 1 479 483 annotation2392297 1 CynR range2392297 1 1270 2169 annotation2392295 1 CynT range2392295 1 505 1161 BBa_K1393002_sequence 1 tcagaacggtttggtcggcagatatttaccatctaaggtgatgaccgcacgttcgccaccttccgggtccgccactttcttaacgtcgagtttgaagttaatcgcgctaataatgccatcgccaaatttctcatgaaccaacgctttcagggttgtaccgtacacctgcaacatttcatagaaacgatacatcgttgggtcagttggaatacggtcatcaatgcagccacgcagtggaatcatctgcaacagtagaatggagtcttcgtcgagatccagcttcgccccgaccaggcgggcggcgtcggcaggaagcgcctgctgccccagcaaagccgcggttacaaaggcttctgccagaccggtgccgtcggcaatctcggcaaatgagagatcttttttagctttgctgagcaaaatggcatcggcaagatcaagacgaatattgcggttaatttgtgactgaatcatggtggaactcctgatggtttaaaaataaggttacgctgcggtcggttggcgtagcggtatggcacaaacgcgaggattagcggccagtggcacaaactggcgggttgcgccgtcaaaagctgcgatgctgccgctttcaatgtcgtagacccagccgtgcagggcgatccgcccctcttcgagcgccaggcgcaccgatggatgagtttgcaaattagccaactgagcaatgacgttttcacgtaccatcgccgcagcttttgacggtaaatcggaatgcgggcgcgcctcattaacgacgcgggctgaatcggcataacgcagccagtgggagacggcaggcatatggtccatgcactgacagctggcaatggcggtcatcgcgccacagttggaatgaccacaaatcacaatgtcagatacccgaagcgcagcgacggcatactccaccgaagcagaaacgccaccgggttccggcccgtaggaagggacgatattgcccgcgttgcgaataacgaacagatcgccaggctcacgttgcgtcaccagctcagggaccagacggctgtcggagcaggagataaaaagtgtgcgcgggctttgctgtgtcgccagctgtttaaacaaggcttcccgcttcggaaatgcctcgcgctggaatttaaggaatccatcaataatctctttcaccgttaacctctgtctgtctctggaatgagaggcagactactcgccgattgtcataaggtaaaagtctcatttatgatgagttccattggatttacttataggttgcgaatgctctctcgacatatcaattattttcttgccgtggctgaacatggcagcttcacccgtgccgccagtgcgttgcacgtctcccagcctgcgctttcccagcagattcgccagttagaggagagtttaggcgtgccgctgtttgaccgtagcgggcgaacgattcgtctcactgatgcaggagaagtctggcgacagtacgccagccgggcgttacaggaactgggggcgggtaaacgggcgattcatgatgttgccgatctgacgcgaggatcgctgcgtatcgccgtcacccccacctttacgagctactttatcggccccttaatggcggatttctatgcgcgctatcccagcatcacgctccagctacaggaaatgtcgcaggagaaaatcgaggatatgctttgccgcgacgagttggacgttgggattgccttcgcgcctgtgcattcgccggagctggaggcaattcctttactgacagaaagtttagcgttagtcgtggcgcaacatcatccgctggccgtccatgaacaggtggcgttgagtcgcttgcatgatgaaaaactggtcctgctcagcgcggaatttgccaccagagagcaaattgaccactactgcgagaaagcggggctacatccacaggtggtcattgaggcgaactcaattagcgcggttctggagctgattcgccgcacttccctttccacattgttaccagcagcgattgccacacaacatgacgggcttaaagctatttctcttgccccgccactactggagagaacggcggttttgttgcggcggaaaaatagctggcagacagccgccgcgaaggcatttttgcacatggcgttggataaatgcgcggttgttggcggaaatgaatcacggtag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z