BBa_K1394001
1
BBa_K1394001
Linear Magainin 1 peptide
2014-10-04T11:00:00Z
2016-02-03T10:54:34Z
The sequence for Magainin 1 was generated by reverse translating the amino acid sequence for the peptide and then codon optimising it. This gene was then synthesised. The SUMO protein fusion partner was derived from the SUMO biobrick introduced by TU Delft 2013.
This partcodes for their Magainin 1 antimicrobial peptide. It includes a SUMO fusion partner to allow soluble, non-toxic expression of the gene in E. coli.
false
false
_1553_1771_
4206
17889
9
In stock
false
Because Magainin 1 is normally cytotoxic to E. coli and other bacteria, the SUMO fusion partner is critical to successful expression of the construct. Without SUMO, the antimicrobial peptide would likely lyse the cell membrane.
false
Sean Lowe
annotation2427839
1
RBS
range2427839
1
24
27
annotation2427943
1
BBa_K731721
range2427943
1
420
467
annotation2427838
1
T7 promoter
range2427838
1
1
20
annotation2427946
1
T7 terminator
range2427946
1
420
467
annotation2427945
1
Magainin I Antimicrobial Peptide
range2427945
1
345
419
annotation2427944
1
HIS-tagged SUMO Protein
range2427944
1
33
344
annotation2427836
1
BBa_K525998
range2427836
1
1
32
annotation2427837
1
B0034
range2427837
1
21
32
BBa_K1394001_sequence
1
taatacgactcactatagggaaagaggagaaaatgcatcatcatcatcatcattcggactcagaagtcaatcaagaagctaagccagaggtcaagccagaagtcaagcctgagactcacatcaatttaaaggtgtccgatggatcttcagagatcttcttcaagatcaaaaagaccactcctttaagaaggctgatggaagcgttcgctaaacgtcagggtaaggaaatggactccttaagattcttgtacgacggtattaggattcaagctgatcagacccctgaagatttggacatggatgataacgatattattgaggctcacagagaacagaccggtggtggtattggtaaatttctgcatagcgcaggtaaatttggtaaagcctttgtgggcgaaatcatgaaaagctgataactagcataaccccttggggcctctaaacgggtcttgaggggttttttg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z