BBa_K896000 1 BBa_K896000 SQR(sulfide quinone reductase),from Synechococcus sp. PCC 7002 plasmid pAQ7 2012-09-08T11:00:00Z 2015-05-08T01:13:41Z Synechococcus sp. PCC 7002 (strain: PCC 7002; ATCC 27264) Location : plasmid: pAQ7 Sequence : NC_010474.1 (85060..86340) AAAAABBBBBCCCCCC false true _1161_ 0 12739 9 In stock true DDDDDDEEEEEFFFFFF false Hsun-I Chiu annotation2182604 1 start range2182604 1 1 3 annotation2182658 1 K896000 range2182658 1 1 1281 annotation2182605 1 stop range2182605 1 1279 1281 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_K1395002 1 BBa_K1395002 sqr gene (sulfide quinone reductase) under constitutive promoter 2014-10-09T11:00:00Z 2015-05-08T01:10:15Z Biobrick Bba_K896000 and Constitutive Promoter (BBA_J23119) The sqr gene encodes a protein of 427 amino acid residues with a theoretical molecular weight of 47 kDa. The sqr gene is expressed under a constitutive promoter and this enzyme converts the sulphide (S-2) to elemental Sulfur. Its expression is based on the availability of RNA polymerase holoenzyme and the expression is not affected by any transcription factors and is 3A assembly (RFC10) compatible. The Constitutive Promoter (BBA_J23119) is the "consensus" promoter sequence and the strongest member of the constitutive promoter family developed by John Christopher Anderson of UC Berkeley. This promoter can be used to tunes the expression level of constitutively expressed parts. This part codes for the protein sulfide quinone reductase which is a FAD dependent oxidoreductase. Sulfide-quinone reductase (SQR),an ancient flavoprotein, is obligatory for growth on sulfide as hydrogen donor in photo and chemolithoautotrophic bacteria. It is a unique enzyme which is responsible for transfer of electrons from sulfide into the quinone pool. This enzyme converts the sulphide to Sulfur by the reaction. false false _1772_ 0 20258 9 It's complicated false During designing we used RFC10 assembly to create this biobrick by the use of previously existing biobricks. As per the protocol of RFC 10 we were constrained to use pSB1K3 as our backbone to eliminate the chances of false clone as our Part A and Part B were present in pSB1C3. Once cloning was done we digested our clone with EcoR1 and Pst1, and ligated it with pSB1C3 to standardise our part. false Gaurav Sinsinbar,Nikhil Patidar ,Chandel Angad Singh component2414756 1 BBa_K896000 component2414752 1 BBa_J23119 annotation2414756 1 BBa_K896000 range2414756 1 42 1322 annotation2414752 1 BBa_J23119 range2414752 1 1 35 BBa_K1395002_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagatggctcatattgttgtaatcggtgctggtattggtggtctgccgacggcctatgaactgaggcacctgttgactaccgaccacactgttaccctcattgctgatacaccccactttacgtttatcccttctttgccctgggtggcccttggtctgaaaagattggatcaggtgcaattacccttgccaacgttggcccgtcgccatggtctgcattgggtacagggggctgtgcagcagattaatccccaggaaaggcatgttttggttgggtcagacgcaaaacgaattgactatgactatgtggtgatcgcaccgggcgcagctttaaatctcgatgctttaccgggtctcggtccagagacaggctttacccagtcagtttgtaatccccaccatgcactgcttgcccatgaagcttgggaaaaatttatccagaatccaggcccgttggtcgtgggggcggcaccgggggcgagttgttttggcccggcctatgagtttgcgttgttggcagattggcaactgcgacggttgggtttgcgggagcaggtaccgatcacattggtcaccccagaaccctatttgggtcatttggggattgggggcatggcccactcgcaggaattggttgagcaggttctccagcaacaggatattgcgacccgtgccaatgctgagattaccgccattaaaccggatatgattggtttggcggatggggaacagctaccctttgcctacagtatggttttgccgtcgttccaggggcctgcttttttgcgtgactgtccggcgatctccaattcccaggggtttattcccgtcttgcccacctatcaacatccagcgttcgattccgtgtatgcggcgggggtgattgttgaactaacgccccatgaagccacaccaatccccacgggtctaccgaaaacaggacaaatgacggaggcgatggggatggccgccgcccacaatattgcccgacagcttaactccaatctgggtgctccggtcacggcgaccctcgccgcaatttgtatgagtgattttggcgatcgcggcattatcttcatcgccgatccggtacagcgggagccaggcatggtcaaacgtcgccgctgtgtcgccctcgaaggacggtgggtgagttggagcaaaaccctttttgaactgtttttcctgacgaaaatgcgttggggtctaacgataccttggtttgagaaactgggcttaaagacattgggattgcagcttgtgcgtccccttcctccagactaa BBa_K896000_sequence 1 atggctcatattgttgtaatcggtgctggtattggtggtctgccgacggcctatgaactgaggcacctgttgactaccgaccacactgttaccctcattgctgatacaccccactttacgtttatcccttctttgccctgggtggcccttggtctgaaaagattggatcaggtgcaattacccttgccaacgttggcccgtcgccatggtctgcattgggtacagggggctgtgcagcagattaatccccaggaaaggcatgttttggttgggtcagacgcaaaacgaattgactatgactatgtggtgatcgcaccgggcgcagctttaaatctcgatgctttaccgggtctcggtccagagacaggctttacccagtcagtttgtaatccccaccatgcactgcttgcccatgaagcttgggaaaaatttatccagaatccaggcccgttggtcgtgggggcggcaccgggggcgagttgttttggcccggcctatgagtttgcgttgttggcagattggcaactgcgacggttgggtttgcgggagcaggtaccgatcacattggtcaccccagaaccctatttgggtcatttggggattgggggcatggcccactcgcaggaattggttgagcaggttctccagcaacaggatattgcgacccgtgccaatgctgagattaccgccattaaaccggatatgattggtttggcggatggggaacagctaccctttgcctacagtatggttttgccgtcgttccaggggcctgcttttttgcgtgactgtccggcgatctccaattcccaggggtttattcccgtcttgcccacctatcaacatccagcgttcgattccgtgtatgcggcgggggtgattgttgaactaacgccccatgaagccacaccaatccccacgggtctaccgaaaacaggacaaatgacggaggcgatggggatggccgccgcccacaatattgcccgacagcttaactccaatctgggtgctccggtcacggcgaccctcgccgcaatttgtatgagtgattttggcgatcgcggcattatcttcatcgccgatccggtacagcgggagccaggcatggtcaaacgtcgccgctgtgtcgccctcgaaggacggtgggtgagttggagcaaaaccctttttgaactgtttttcctgacgaaaatgcgttggggtctaacgataccttggtttgagaaactgggcttaaagacattgggattgcagcttgtgcgtccccttcctccagactaa BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z