BBa_K1396000 1 BBa_K1396000 pSB1C3: 2XStrep_Flagtag_LL-37_Mussel Foot Protein_sfGFP 2014-10-09T11:00:00Z 2016-02-03T10:06:09Z Synthesized in lab The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action. false false _1773_ 4206 22908 9 It's complicated false We capped the LL-37 Domain which is otherwise toxic to cells with a removable strep tag that can be used for column chromatography. Additionally there are linker units between individual components false Ariel Hernandez-Leyva annotation2414636 1 sfGFP range2414636 1 1034 1701 annotation2414630 1 Mfp 1-5-1 range2414630 1 260 591 annotation2414624 1 2XStrepFlag Tag range2414624 1 2 121 annotation2414627 1 LL-37 range2414627 1 122 239 BBa_K1396000_sequence 1 atgtcagcctggagtcatccgcagttcgagaagggcgggggcagtggcgggggctcaggcggtagtgcttggtcgcacccacagtttgaaaaaagtgctgattataaagacgatgatgacaaactgctgggtgacttcttccgtaaatctaaagaaaaaatcggtaaagaatttaaacgtatcgttcagcgtatcaaagacttcctgcgtaacctggttccgcgtaccgaactcgagagcggtggcagcgcaggcggttccggttctggctctagcggcgggtcttccggagcgtcgggtactggaaccgcgggtggtactggtagcgggtctggcactggctccggtaccgcgaaaccgagctatccgccgacctagaaagcgaagcctagctatccgcctacctagaaagccaaaccgagttacccacctacttagaaagctaaaccttcgtatcctccgacctagaaagcgaaaccgagttaccctccgacgtagaaagctaagccgagctacccaccaacgtagaagtcttctgaagaatagaaaggtggttagtagccgggtaacacctagcactagcactctggtggttcttagcacggttctggttagcacggtggttagaaaggtaaatagtagggtaaagctaaaaaatagtagtagaaatagaaaaactctggtaaatagaaatagctgaaaaaagctcgtaaatagcaccgtaaaggttagaaaaaatagtagggtggtggttcttctatggccaaaccatcatacccgccgacttagaaagcgaaacccagttacccgccgacgtagaaagcaaaaccgtcctacccaccaacctagaaagccaaaccgagctatccacctacgtagaaagccaaacctagctacccaccgacctagaaagcaaaaccgtcctacccgcccacgtagaaagggcccggtggttctgggggaggtagcggcggtggatctggtgcggccgcacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z