BBa_K1396001 1 BBa_K1396001 pSB1C3: LL-37_MFP 2014-10-09T11:00:00Z 2015-05-08T01:10:15Z Synthesized in lab. he part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action. false false _1773_ 0 22908 9 It's complicated false We capped the LL-37 Domain which is otherwise toxic to cells with a removable strep tag that can be used for column chromatography. Additionally there are linker units between individual components false Ariel Hernandez-Leyva annotation2414644 1 Strep-flag Tag range2414644 1 1 123 annotation2414643 1 LL-37 range2414643 1 124 231 annotation2414649 1 mfp 1-5-1 range2414649 1 351 942 BBa_K1396001_sequence 1 atgtcagcctggagtcatccgcagttcgagaagggcgggggcagtggcgggggctcaggcggtagtgcttggtcgcacccacagtttgaaaaaagtgctgattataaagacgatgatgacaaactgctgggtgacttcttccgtaaatctaaagaaaaaatcggtaaagaatttaaacgtatcgttcagcgtatcaaagacttcctgcgtaacctggttccgcgtaccgaactcgagagcggtggcagcgcaggcggttccggttctggctctagcggcgggtcttccggagcgtcgggtactggaaccgcgggtggtactggtagcgggtctggcactggctccggtaccgcgaaaccgagctatccgccgacctagaaagcgaagcctagctatccgcctacctagaaagccaaaccgagttacccacctacttagaaagctaaaccttcgtatcctccgacctagaaagcgaaaccgagttaccctccgacgtagaaagctaagccgagctacccaccaacgtagaagtcttctgaagaatagaaaggtggttagtagccgggtaacacctagcactagcactctggtggttcttagcacggttctggttagcacggtggttagaaaggtaaatagtagggtaaagctaaaaaatagtagtagaaatagaaaaactctggtaaatagaaatagctgaaaaaagctcgtaaatagcaccgtaaaggttagaaaaaatagtagggtggtggttcttctatggccaaaccatcatacccgccgacttagaaagcgaaacccagttacccgccgacgtagaaagcaaaaccgtcctacccaccaacctagaaagccaaaccgagctatccacctacgtagaaagccaaacctagctacccaccgacctagaaagcaaaaccgtcctacccgcccacgtagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z