LOCUS       BBa_K1398001            1428 bp    DNA     linear       22-Nov-2024
DEFINITION  XenB (Inducible Construct)
ACCESSION   BBa_K1398001
VERSION     BBa_K1398001.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1291
                     /label=BBa_K1398000
     stem_loop       1311..1354
                     /label=stem_loop
     misc_feature    1300..1379
                     /label=BBa_B0010
     terminator      1300..1379
                     /label=BBa_B0010
     stem_loop       1395..1414
                     /label=T7 TE
     misc_feature    1421..1421
                     /label=stop
     polyA_site      1415..1428
                     /label=polya
     misc_feature    1388..1428
                     /label=BBa_B0012
     terminator      1388..1428
                     /label=BBa_B0012
     terminator      1300..1428
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cgacgatctt
      241 tgatccgatc aaactgggtg acctggaact gagcaaccgt atcattatgg cgccgttaac
      301 gcgttgccgt gcggacgagg gtcgtgtgcc gaacgcgctg atggccgagt actacgttca
      361 gcgtgcgagc gccggtctga tcctgtccga ggcgaccagc gttaccccga tgggcgtggg
      421 ctacccggac accccgggta tttggagcaa tgaccaagtg cgcggttgga ctaacatcac
      481 gaaagctgtc catgcggctg gcggcaagat tgtgctgcaa ctgtggcatg tcggtcgcat
      541 cagccacccg ctgtacttga acggcgaggc gccagtcgcc ccgagcgcga tccagccgaa
      601 gggtcacgtg agcctggtcc gtccgttggc ggattacccg accccgcgtg cattggaaac
      661 ggcagaaatc gccgagattg tcgaagcata ccgcacgggc gcagagaatg ctaaagccgc
      721 aggctttgac ggtgttgaga tccacggcgc gaatggttat ctgctggatc agttccttca
      781 atcgtctacc aaccaacgca ccgacaatta tggtggtagc ctggagaatc gtgcgcgtct
      841 gctgctggaa gtgaccgacg cagcgattga tgtttggggt gccggtcgcg tcggtgttca
      901 cctggcccct cgtgcagata gccacgacat gggcgacgat aacctggcgg aaactttcac
      961 gtatgtagcg cgtgagctgg gcaagcgcgg tattgcgttc atttgtagcc gcgagaaaga
     1021 gggtgcagat tctctgggtc cgcagctcaa agaggcattt ggtggcgcgt acattgcgaa
     1081 tgaacgcttc accaaagatt ccgcaaacgc ttggctggcg gaaggcaaag ccgatgctgt
     1141 ggcgttcggc gttccgttta tcgcaaaccc ggacttgcca gcgcgtctga aagctgatgc
     1201 cccgttgaat gaaccgcgtc cggaactgtt ctatggtaag ggtcctgttg gctacattga
     1261 ctatccgacc ctgcaccatc accatcatca ctactagagc caggcatcaa ataaaacgaa
     1321 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1381 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//