BBa_K1398002 1 BBa_K1398002 NemA (N-ethylmaleimide reductase) 2014-08-10T11:00:00Z 2015-05-08T01:10:15Z Source of original sequence: Escherichia coli O104:H4 str. C227-11 N-ethylmaleimide reductase, created for use as a Trinitroltoluene and Nitroglycerine degrading protein. NemA is a flavoprotein that primarily catalyses the reduction of N-ethylmaleimide (NEM), which is toxic to cell growth. However, it is also involved in the degradation of other toxic compounds for their reuse in nitrogen metabolism. Some of these compounds include PETN, quinones and chromate. It increases the resistance of organisms to the toxic effects of these compounds. This sequence only encodes for the protein, an attached His (x6) Tag to allow for purification and a double-STOP codon. It therefore requires regulatory sequences (promoter/RBS/terminator) to be added for expression. The protein has been codon-optimised for expression in E. coli. false false _1776_ 0 19951 9 Not in stock false The original sequence of this part was found using the NCBI database and taken from E. coli. The sequence was then codon-optimised to ensure increased expression. This has resulted in a novel synthetic sequence. false Exeter iGEM 2014 BBa_K1398002_sequence 1 atgtctagcgaaaaactgtacagcccgctgaaagttggtgcaattaccgccgcaaatcgtatcttcatggcgcctctgacccgtctgcgctctatcgagccaggcgacatcccgacgccgctgatggcagagtattatcgtcagcgtgcatccgccggtctgatcatcagcgaagcaacccagattagcgcccaggcgaagggttacgcgggtgctccgggcattcatagcccggagcaaattgcggcgtggaagaaaatcaccgcaggcgtacacgcggagaatggccacatggctgtgcaactgtggcatacgggtcgtattagccatgcatcgctgcaaccgggtggccaagcaccggtggcacctagcgccttgagcgcgggtacgcgcacgtccctgcgcgatgagaacggtcaggcaatccgtgtcgagactagcatgccacgcgcgctggaattggaagaaatcccgggtatcgttaatgatttccgccaagcgattgcaaacgcgcgtgaggccggcttcgacctggtggaattgcacagcgcgcacggttacttgctgcatcagtttctgagcccgtcctcaaatcatcgtaccgatcagtatggcggcagcgtcgagaatcgtgcgcgtctggttctggaagttgtggatgcgggcattgaagagtggggtgcggaccgcattggcatccgcgtcagcccgatcggtacctttcagaacaccgacaacggtccgaacgaagaggcagacgctctgtacctgatcgagcaactgggtaagcgcggtattgcgtacttacacatgagcgagccggactgggccggtggcgagccgtataccgatgcgttccgtgaaaaagtgagagcgcgctttcacggtccgattattggtgcgggtgcctatacggttgagaaagctgaaacgcttatcggtaagggtctgattgacgctgtcgcgttcggccgtgattggattgcgaacccggatctcgttgcgcgtctgcaacgtaaagccgacctgaatccgcagcgtgctgagagcttttacggtggcggcgccgaaggctacaccgactacccgactctgcatcaccatcaccaccac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z