LOCUS       BBa_K1398003            1476 bp    DNA     linear       29-Jun-2024
DEFINITION  NemA (Inducible Construct)
ACCESSION   BBa_K1398003
VERSION     BBa_K1398003.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1339
                     /label=BBa_K1398002
     stem_loop       1359..1402
                     /label=stem_loop
     misc_feature    1348..1427
                     /label=BBa_B0010
     terminator      1348..1427
                     /label=BBa_B0010
     stem_loop       1443..1462
                     /label=T7 TE
     misc_feature    1469..1469
                     /label=stop
     polyA_site      1463..1476
                     /label=polya
     misc_feature    1436..1476
                     /label=BBa_B0012
     terminator      1436..1476
                     /label=BBa_B0012
     terminator      1348..1476
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ctagcgaaaa
      241 actgtacagc ccgctgaaag ttggtgcaat taccgccgca aatcgtatct tcatggcgcc
      301 tctgacccgt ctgcgctcta tcgagccagg cgacatcccg acgccgctga tggcagagta
      361 ttatcgtcag cgtgcatccg ccggtctgat catcagcgaa gcaacccaga ttagcgccca
      421 ggcgaagggt tacgcgggtg ctccgggcat tcatagcccg gagcaaattg cggcgtggaa
      481 gaaaatcacc gcaggcgtac acgcggagaa tggccacatg gctgtgcaac tgtggcatac
      541 gggtcgtatt agccatgcat cgctgcaacc gggtggccaa gcaccggtgg cacctagcgc
      601 cttgagcgcg ggtacgcgca cgtccctgcg cgatgagaac ggtcaggcaa tccgtgtcga
      661 gactagcatg ccacgcgcgc tggaattgga agaaatcccg ggtatcgtta atgatttccg
      721 ccaagcgatt gcaaacgcgc gtgaggccgg cttcgacctg gtggaattgc acagcgcgca
      781 cggttacttg ctgcatcagt ttctgagccc gtcctcaaat catcgtaccg atcagtatgg
      841 cggcagcgtc gagaatcgtg cgcgtctggt tctggaagtt gtggatgcgg gcattgaaga
      901 gtggggtgcg gaccgcattg gcatccgcgt cagcccgatc ggtacctttc agaacaccga
      961 caacggtccg aacgaagagg cagacgctct gtacctgatc gagcaactgg gtaagcgcgg
     1021 tattgcgtac ttacacatga gcgagccgga ctgggccggt ggcgagccgt ataccgatgc
     1081 gttccgtgaa aaagtgagag cgcgctttca cggtccgatt attggtgcgg gtgcctatac
     1141 ggttgagaaa gctgaaacgc ttatcggtaa gggtctgatt gacgctgtcg cgttcggccg
     1201 tgattggatt gcgaacccgg atctcgttgc gcgtctgcaa cgtaaagccg acctgaatcc
     1261 gcagcgtgct gagagctttt acggtggcgg cgccgaaggc tacaccgact acccgactct
     1321 gcatcaccat caccaccact actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1381 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1441 ctggctcacc ttcgggtggg cctttctgcg tttata
//