BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961225 1 -10 range1961225 1 161 166 annotation1961224 1 -35 range1961224 1 137 142 annotation1961227 1 start range1961227 1 173 173 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 BBa_K1398002 1 BBa_K1398002 NemA (N-ethylmaleimide reductase) 2014-08-10T11:00:00Z 2015-05-08T01:10:15Z Source of original sequence: Escherichia coli O104:H4 str. C227-11 N-ethylmaleimide reductase, created for use as a Trinitroltoluene and Nitroglycerine degrading protein. NemA is a flavoprotein that primarily catalyses the reduction of N-ethylmaleimide (NEM), which is toxic to cell growth. However, it is also involved in the degradation of other toxic compounds for their reuse in nitrogen metabolism. Some of these compounds include PETN, quinones and chromate. It increases the resistance of organisms to the toxic effects of these compounds. This sequence only encodes for the protein, an attached His (x6) Tag to allow for purification and a double-STOP codon. It therefore requires regulatory sequences (promoter/RBS/terminator) to be added for expression. The protein has been codon-optimised for expression in E. coli. false false _1776_ 0 19951 9 Not in stock false The original sequence of this part was found using the NCBI database and taken from E. coli. The sequence was then codon-optimised to ensure increased expression. This has resulted in a novel synthetic sequence. false Exeter iGEM 2014 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K1398003 1 NemA NemA (Inducible Construct) 2014-08-10T11:00:00Z 2015-05-08T01:10:15Z The original source of NemA was E. coli. The regulatory sequences were acquired from the iGEM repository. A construct created to degraded TNT and nitroglycerine. The construct contains the coding sequence for NemA (BBa_K1398002), an enzyme involved in the degradation of toxic compounds for their reuse in nitrogen metabolism. The construct also contains an inducible promoter (BBa_R0010), a strong RBS (BBa_B0034) and a double terminator (BBa_B0010). The protein has been codon-optimised for expression in E. coli. false false _1776_ 0 19951 9 It's complicated false This construct was created to allow for the over-expression of NemA, so it contains high expression regulatory sequences. A lactose inducible promoter has been used to give control over expression. false Exeter iGEM 2014 component2380772 1 BBa_K1398002 component2380779 1 BBa_B0015 component2380771 1 BBa_B0034 component2380763 1 BBa_R0010 annotation2380763 1 BBa_R0010 range2380763 1 1 200 annotation2380771 1 BBa_B0034 range2380771 1 209 220 annotation2380772 1 BBa_K1398002 range2380772 1 227 1339 annotation2380779 1 BBa_B0015 range2380779 1 1348 1476 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1398003_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgtctagcgaaaaactgtacagcccgctgaaagttggtgcaattaccgccgcaaatcgtatcttcatggcgcctctgacccgtctgcgctctatcgagccaggcgacatcccgacgccgctgatggcagagtattatcgtcagcgtgcatccgccggtctgatcatcagcgaagcaacccagattagcgcccaggcgaagggttacgcgggtgctccgggcattcatagcccggagcaaattgcggcgtggaagaaaatcaccgcaggcgtacacgcggagaatggccacatggctgtgcaactgtggcatacgggtcgtattagccatgcatcgctgcaaccgggtggccaagcaccggtggcacctagcgccttgagcgcgggtacgcgcacgtccctgcgcgatgagaacggtcaggcaatccgtgtcgagactagcatgccacgcgcgctggaattggaagaaatcccgggtatcgttaatgatttccgccaagcgattgcaaacgcgcgtgaggccggcttcgacctggtggaattgcacagcgcgcacggttacttgctgcatcagtttctgagcccgtcctcaaatcatcgtaccgatcagtatggcggcagcgtcgagaatcgtgcgcgtctggttctggaagttgtggatgcgggcattgaagagtggggtgcggaccgcattggcatccgcgtcagcccgatcggtacctttcagaacaccgacaacggtccgaacgaagaggcagacgctctgtacctgatcgagcaactgggtaagcgcggtattgcgtacttacacatgagcgagccggactgggccggtggcgagccgtataccgatgcgttccgtgaaaaagtgagagcgcgctttcacggtccgattattggtgcgggtgcctatacggttgagaaagctgaaacgcttatcggtaagggtctgattgacgctgtcgcgttcggccgtgattggattgcgaacccggatctcgttgcgcgtctgcaacgtaaagccgacctgaatccgcagcgtgctgagagcttttacggtggcggcgccgaaggctacaccgactacccgactctgcatcaccatcaccaccactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa BBa_K1398002_sequence 1 atgtctagcgaaaaactgtacagcccgctgaaagttggtgcaattaccgccgcaaatcgtatcttcatggcgcctctgacccgtctgcgctctatcgagccaggcgacatcccgacgccgctgatggcagagtattatcgtcagcgtgcatccgccggtctgatcatcagcgaagcaacccagattagcgcccaggcgaagggttacgcgggtgctccgggcattcatagcccggagcaaattgcggcgtggaagaaaatcaccgcaggcgtacacgcggagaatggccacatggctgtgcaactgtggcatacgggtcgtattagccatgcatcgctgcaaccgggtggccaagcaccggtggcacctagcgccttgagcgcgggtacgcgcacgtccctgcgcgatgagaacggtcaggcaatccgtgtcgagactagcatgccacgcgcgctggaattggaagaaatcccgggtatcgttaatgatttccgccaagcgattgcaaacgcgcgtgaggccggcttcgacctggtggaattgcacagcgcgcacggttacttgctgcatcagtttctgagcccgtcctcaaatcatcgtaccgatcagtatggcggcagcgtcgagaatcgtgcgcgtctggttctggaagttgtggatgcgggcattgaagagtggggtgcggaccgcattggcatccgcgtcagcccgatcggtacctttcagaacaccgacaacggtccgaacgaagaggcagacgctctgtacctgatcgagcaactgggtaagcgcggtattgcgtacttacacatgagcgagccggactgggccggtggcgagccgtataccgatgcgttccgtgaaaaagtgagagcgcgctttcacggtccgattattggtgcgggtgcctatacggttgagaaagctgaaacgcttatcggtaagggtctgattgacgctgtcgcgttcggccgtgattggattgcgaacccggatctcgttgcgcgtctgcaacgtaaagccgacctgaatccgcagcgtgctgagagcttttacggtggcggcgccgaaggctacaccgactacccgactctgcatcaccatcaccaccac BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z