BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K660004 1 BBa_K660004 iLOV 2011-09-15T11:00:00Z 2015-05-08T01:13:03Z iLOV is a version of LOV2 that has been altered through site directed mutagenesis and DNA shuffling. Released HQ 2013 iLOV has the same function and uses as LOV2. As a reporter, it is advantageous over GFP derived fluorescent proteins due to its small size (useful if you are tagging proteins), ability to recover quickly from photobleaching and use in anoxic conditions. false false _837_ 0 9421 9 In stock true illegal restriction sites. The part was synthesized to remove them. false Glasgow Uni 2011 annotation2135615 1 iLOV Domain range2135615 1 1 336 BBa_K1398008 1 NemR (RP) NemR Recognition Promoter 2014-08-11T11:00:00Z 2015-05-08T01:10:15Z The origin organism of the NemR box is Escherichia coli JD22799. The high level constitutive promoter originates from the iGEM repository. This sequences combines a high level constitutive promoter with the NemR binding box, which allows NemR to bind to DNA when no TNT is present in the cell. It was created for use as a TNT-detection mechanism. It combines BBa_J231000 with the NemR box to create a promoter that will theoretically have high levels of transcription when TNT is not present. false false _1776_ 0 19951 9 Not in stock false This a completely original part, created from two distinct regulatory sequences. It was created using standard sequence design software. false Exeter iGEM 2014 BBa_K1398007 1 NemR PRep NemR Promoter Reporter 2014-08-11T11:00:00Z 2015-05-08T01:10:15Z The origin of the NemR box is Escherichia coli JD22799. The regulatory sequences were acquired from the iGEM repository. A construct created to test the effectiveness of the NemR recognizing promoter (BBa_1398008). The construct begins with the synthetic promoter NemR, which combines a high-expression promoter with the NemR recognition box (also found in BBa_1398005). It is followed by a strong RBS (BBa_B0034), the fluorescent reporter iLOV (listed in the repository as BBa_K660004), a double STOP codon and a double terminator (BBa_B0015). false false _1776_ 0 19951 9 Not in stock false The promoter used in this construct was synthetically designed and does not exist it nature. iLOV was used as a reporter in this construct as it ???outperforms??? GFP. false Exeter iGEM 2014 component2380801 1 BBa_B0015 component2380792 1 BBa_B0034 component2380794 1 BBa_K660004 component2380790 1 BBa_K1398008 annotation2380790 1 BBa_K1398008 range2380790 1 1 34 annotation2380794 1 BBa_K660004 range2380794 1 61 396 annotation2380792 1 BBa_B0034 range2380792 1 43 54 annotation2380801 1 BBa_B0015 range2380801 1 405 533 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K1398008_sequence 1 ttgacgtagacctcagggtctactataatgctag BBa_K660004_sequence 1 atgattgaaaaaaactttgtgattaccgatccgcgtctgccggataacccgattatttttgcgagcgatggctttctggaactgaccgaatatagccgtgaagaaattctgggccgtaacgcgcgttttttgcagggcccggaaaccgatcaggcgaccgtgcagaaaattcgtgatgcgattcgtgatcagcgtgaaaccaccgtgcagctgattaactataccaaaagcggcaaaaaattttggaacctgctgcatttgcagccggtgcgtgatcagaaaggcgaattgcagtattttattggcgtgcagctggatggcagcgatcatgtgtaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1398007_sequence 1 ttgacgtagacctcagggtctactataatgctagtactagagaaagaggagaaatactagatgattgaaaaaaactttgtgattaccgatccgcgtctgccggataacccgattatttttgcgagcgatggctttctggaactgaccgaatatagccgtgaagaaattctgggccgtaacgcgcgttttttgcagggcccggaaaccgatcaggcgaccgtgcagaaaattcgtgatgcgattcgtgatcagcgtgaaaccaccgtgcagctgattaactataccaaaagcggcaaaaaattttggaacctgctgcatttgcagccggtgcgtgatcagaaaggcgaattgcagtattttattggcgtgcagctggatggcagcgatcatgtgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z