BBa_K1399011 1 BBa_K1399011 Plac-RFP(LVA) 2014-09-18T11:00:00Z 2015-05-08T01:10:16Z other biobricks Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LVA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. This part can be considered as improved version of BBa_J04451 as it does not contain StuI restriction site coding for additional 'RP' residues between RFP and LVA tag. false false _1777_ 0 22477 9 In stock true Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1] false Anna Stikane annotation2386309 1 T7 TE range2386309 1 1069 1088 annotation2386307 1 stem_loop range2386307 1 985 1028 annotation2386289 1 BBa_R0010 range2386289 1 1 200 annotation2386306 1 BBa_B0010 range2386306 1 974 1053 annotation2386311 1 stop range2386311 1 1095 1095 annotation2386292 1 -35 range2386292 1 137 142 annotation2386296 1 BBa_B0034 range2386296 1 209 220 annotation2386298 1 start range2386298 1 227 229 annotation2386308 1 BBa_B0012 range2386308 1 1062 1102 annotation2386297 1 conserved range2386297 1 213 216 annotation2386294 1 LacI binding site range2386294 1 166 200 annotation2386301 1 LVA-ssrA tag range2386301 1 902 934 annotation2386303 1 stop range2386303 1 938 940 annotation2386295 1 start range2386295 1 173 173 annotation2386304 1 Barcode of BBa_E1010 range2386304 1 941 965 annotation2386290 1 end of LacI coding region (inactive) range2386290 1 1 88 annotation2386302 1 stop range2386302 1 935 937 annotation2386305 1 BBa_B0015 range2386305 1 974 1102 annotation2386310 1 polya range2386310 1 1089 1102 annotation2386299 1 BBa_K1399001 range2386299 1 227 940 annotation2386300 1 BBa_E1010 w/o stop range2386300 1 230 901 annotation2386291 1 CAP binding site range2386291 1 89 126 annotation2386293 1 -10 range2386293 1 161 166 BBa_K1399011_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z