##gff-version 3
##sequence-region BBa_K1399012 1 1102
BBa_K1399012	.	start_codon	173	173	.	+	0	ID=annotation2383992;Name=start
BBa_K1399012	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation2383991;Name=LacI binding site
BBa_K1399012	.	engineered_region	209	220	.	+	0	ID=annotation2383994;Name=BBa_B0034
BBa_K1399012	.	stop_codon	935	937	.	+	0	ID=annotation2383998;Name=stop
BBa_K1399012	.	polyA_site	1089	1102	.	+	0	ID=annotation2384005;Name=polya
BBa_K1399012	.	start_codon	227	229	.	+	0	ID=annotation2383995;Name=start
BBa_K1399012	.	promoter	161	166	.	+	0	ID=annotation2383990;Name=-10
BBa_K1399012	.	engineered_region	974	1102	.	+	0	ID=annotation2384007;Name=BBa_B0015
BBa_K1399012	.	conserved_region	213	216	.	+	0	ID=annotation2383993;Name=conserved
BBa_K1399012	.	engineered_region	1062	1102	.	+	0	ID=annotation2384003;Name=BBa_B0012
BBa_K1399012	.	tag	902	934	.	+	0	ID=annotation2383997;Name=tag
BBa_K1399012	.	engineered_region	1	200	.	+	0	ID=annotation2383986;Name=BBa_R0010
BBa_K1399012	.	promoter	137	142	.	+	0	ID=annotation2383989;Name=-35
BBa_K1399012	.	engineered_region	227	940	.	+	0	ID=annotation2384000;Name=BBa_K1399002
BBa_K1399012	.	stem_loop	1069	1088	.	+	0	ID=annotation2384004;Name=T7 TE
BBa_K1399012	.	stop_codon	1095	1095	.	+	0	ID=annotation2384006;Name=stop
BBa_K1399012	.	sequence_feature	941	965	.	+	0	ID=annotation2386312;Name=barcode from BBa_E1010
BBa_K1399012	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation2383988;Name=CAP binding site
BBa_K1399012	.	engineered_region	974	1053	.	+	0	ID=annotation2384001;Name=BBa_B0010
BBa_K1399012	.	stem_loop	985	1028	.	+	0	ID=annotation2384002;Name=stem_loop
BBa_K1399012	.	CDS	1	88	.	+	0	ID=annotation2383987;Name=end of LacI coding region (inactive)
BBa_K1399012	.	stop_codon	938	940	.	+	0	ID=annotation2383999;Name=stop
BBa_K1399012	.	CDS	230	901	.	+	0	ID=annotation2383996;Name=RFP
>BBa_K1399012
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctc
cgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaa
ggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcg
cttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacct
gaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggtta
tgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaa
aatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcag
ctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacg
aacgtgctgaaggtcgtcactccaccggtgctgctgcaaacgacgaaaactacgctctggctgcttaataacgctgata
gtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata