BBa_K1399012 1 BBa_K1399012 Plac-RFP(LAA) 2014-09-18T11:00:00Z 2015-05-08T01:10:16Z other biobricks Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LAA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. false false _1777_ 0 22477 9 In stock true Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1] false Anna Stikane annotation2383995 1 start range2383995 1 227 229 annotation2384006 1 stop range2384006 1 1095 1095 annotation2383991 1 LacI binding site range2383991 1 166 200 annotation2384007 1 BBa_B0015 range2384007 1 974 1102 annotation2386312 1 barcode from BBa_E1010 range2386312 1 941 965 annotation2383996 1 RFP range2383996 1 230 901 annotation2383992 1 start range2383992 1 173 173 annotation2383990 1 -10 range2383990 1 161 166 annotation2384003 1 BBa_B0012 range2384003 1 1062 1102 annotation2383988 1 CAP binding site range2383988 1 89 126 annotation2384000 1 BBa_K1399002 range2384000 1 227 940 annotation2384002 1 stem_loop range2384002 1 985 1028 annotation2384004 1 T7 TE range2384004 1 1069 1088 annotation2383993 1 conserved range2383993 1 213 216 annotation2383994 1 BBa_B0034 range2383994 1 209 220 annotation2383997 1 tag range2383997 1 902 934 annotation2383986 1 BBa_R0010 range2383986 1 1 200 annotation2383989 1 -35 range2383989 1 137 142 annotation2384001 1 BBa_B0010 range2384001 1 974 1053 annotation2383987 1 end of LacI coding region (inactive) range2383987 1 1 88 annotation2383999 1 stop range2383999 1 938 940 annotation2383998 1 stop range2383998 1 935 937 annotation2384005 1 polya range2384005 1 1089 1102 BBa_K1399012_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctgctgcaaacgacgaaaactacgctctggctgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z