LOCUS       BBa_K1399013            1102 bp    DNA     linear       10-May-2024
DEFINITION  Plac-RFP(DAS)
ACCESSION   BBa_K1399013
VERSION     BBa_K1399013.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=BBa_R0010
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     misc_feature    213..216
                     /label=conserved
     misc_feature    209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=start
     CDS             230..901
                     /label=RFP
     misc_feature    902..934
                     /label=DAS-ssrA tag
     misc_feature    935..937
                     /label=stop
     misc_feature    938..940
                     /label=stop
     misc_feature    227..940
                     /label=BBa_K1399003
     misc_feature    974..1053
                     /label=BBa_B0010
     stem_loop       985..1028
                     /label=stem_loop
     misc_feature    1062..1102
                     /label=BBa_B0012
     stem_loop       1069..1088
                     /label=T7 TE
     polyA_site      1089..1102
                     /label=polya
     misc_feature    1095..1095
                     /label=stop
     misc_feature    974..1102
                     /label=BBa_B0015
     misc_feature    941..965
                     /label=barcode from BBa_E1010
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 tgctgcaaac gacgaaaact acgctgacgc ttcttaataa cgctgatagt gctagtgtag
      961 atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1021 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     1081 ggtgggcctt tctgcgttta ta
//