BBa_K1399013 1 BBa_K1399013 Plac-RFP(DAS) 2014-09-18T11:00:00Z 2015-05-08T01:10:16Z other biobricks Lactose/IPTG inducible promoter with RFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged RFP is degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation. false false _1777_ 0 22477 9 In stock true Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1] false Anna Stikane annotation2386313 1 barcode from BBa_E1010 range2386313 1 941 965 annotation2384033 1 BBa_B0015 range2384033 1 974 1102 annotation2384028 1 stem_loop range2384028 1 985 1028 annotation2384032 1 stop range2384032 1 1095 1095 annotation2384012 1 BBa_R0010 range2384012 1 1 200 annotation2384024 1 stop range2384024 1 935 937 annotation2384029 1 BBa_B0012 range2384029 1 1062 1102 annotation2384013 1 end of LacI coding region (inactive) range2384013 1 1 88 annotation2384016 1 -10 range2384016 1 161 166 annotation2384030 1 T7 TE range2384030 1 1069 1088 annotation2384023 1 DAS-ssrA tag range2384023 1 902 934 annotation2384021 1 start range2384021 1 227 229 annotation2384026 1 BBa_K1399003 range2384026 1 227 940 annotation2384019 1 conserved range2384019 1 213 216 annotation2384022 1 RFP range2384022 1 230 901 annotation2384018 1 start range2384018 1 173 173 annotation2384027 1 BBa_B0010 range2384027 1 974 1053 annotation2384017 1 LacI binding site range2384017 1 166 200 annotation2384015 1 -35 range2384015 1 137 142 annotation2384025 1 stop range2384025 1 938 940 annotation2384014 1 CAP binding site range2384014 1 89 126 annotation2384020 1 BBa_B0034 range2384020 1 209 220 annotation2384031 1 polya range2384031 1 1089 1102 BBa_K1399013_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctgctgcaaacgacgaaaactacgctgacgcttcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z