LOCUS       BBa_K1399014            1116 bp    DNA     linear       10-May-2024
DEFINITION  Plac-GFP(AAV)
ACCESSION   BBa_K1399014
VERSION     BBa_K1399014.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=start
     CDS             230..940
                     /label=GFP (mut3b)
     misc_feature    941..973
                     /label=AAV-ssrA tag
     misc_feature    974..976
                     /label=stop
     misc_feature    977..979
                     /label=stop
     CDS             227..979
                     /label=BBa_K1399005
     stem_loop       999..1042
                     /label=stem_loop
     misc_feature    988..1067
                     /label=BBa_B0010
     terminator      988..1067
                     /label=BBa_B0010
     stem_loop       1083..1102
                     /label=T7 TE
     misc_feature    1109..1109
                     /label=stop
     polyA_site      1103..1116
                     /label=polya
     misc_feature    1076..1116
                     /label=BBa_B0012
     terminator      1076..1116
                     /label=BBa_B0012
     terminator      988..1116
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc gtaaaggaga
      241 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      301 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      361 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      421 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      481 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      541 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      601 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      661 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      721 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      781 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      841 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      901 agctgctggg attacacatg gcatggatga actatacaaa gctgcaaacg acgaaaacta
      961 cgctgctgct gtttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1021 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1081 ctggctcacc ttcgggtggg cctttctgcg tttata
//