LOCUS       BBa_K1399019             970 bp    DNA     linear       10-May-2024
DEFINITION  Ptet-GFP(AAV)
ACCESSION   BBa_K1399019
VERSION     BBa_K1399019.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..83
                     /label=start
     CDS             84..794
                     /label=GFP (mut3b)
     misc_feature    795..827
                     /label=AAV-ssrA tag
     misc_feature    828..830
                     /label=stop
     misc_feature    831..833
                     /label=stop
     CDS             81..833
                     /label=BBa_K1399005
     stem_loop       853..896
                     /label=stem_loop
     misc_feature    842..921
                     /label=BBa_B0010
     terminator      842..921
                     /label=BBa_B0010
     stem_loop       937..956
                     /label=T7 TE
     misc_feature    963..963
                     /label=stop
     polyA_site      957..970
                     /label=polya
     misc_feature    930..970
                     /label=BBa_B0012
     terminator      930..970
                     /label=BBa_B0012
     terminator      842..970
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      781 atgaactata caaagctgca aacgacgaaa actacgctgc tgctgtttaa taatactaga
      841 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      901 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
      961 tgcgtttata
//