##gff-version 3
##sequence-region BBa_K1399019 1 970
BBa_K1399019	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1399019	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1399019	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1399019	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1399019	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1399019	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1399019	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1399019	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1399019	.	terminator	842	970	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1399019	.	terminator	842	921	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1399019	.	engineered_region	842	921	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1399019	.	stem_loop	853	896	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1399019	.	terminator	930	970	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1399019	.	engineered_region	930	970	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1399019	.	stop_codon	963	963	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1399019	.	stem_loop	937	956	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1399019	.	polyA_site	957	970	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1399019	.	CDS	81	833	.	+	0	ID=BBa_K1399005;Name=BBa_K1399005
BBa_K1399019	.	stop_codon	828	830	.	+	0	ID=annotation2383911;Name=stop;Parent=BBa_K1399005
BBa_K1399019	.	stop_codon	831	833	.	+	0	ID=annotation2383912;Name=stop;Parent=BBa_K1399005
BBa_K1399019	.	CDS	84	794	.	+	0	ID=annotation2383909;Name=GFP (mut3b);Parent=BBa_K1399005
BBa_K1399019	.	tag	795	827	.	+	0	ID=annotation2383910;Name=AAV-ssrA tag;Parent=BBa_K1399005
BBa_K1399019	.	start_codon	81	83	.	+	0	ID=annotation2383908;Name=start;Parent=BBa_K1399005
>BBa_K1399019
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaa
ttttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaac
tacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaa
acagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaac
tacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaag
aagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaa
gaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaa
aatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatc
ccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactata
caaagctgcaaacgacgaaaactacgctgctgctgtttaataatactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttata