LOCUS       BBa_K1399022             976 bp    DNA     linear       10-May-2024
DEFINITION  Ptet-GFP(DAS+2)
ACCESSION   BBa_K1399022
VERSION     BBa_K1399022.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..83
                     /label=start
     CDS             84..794
                     /label=GFP (mut3b)
     misc_feature    795..833
                     /label=NYADAS-ssrA
     misc_feature    834..836
                     /label=stop
     misc_feature    837..839
                     /label=stop
     CDS             81..839
                     /label=BBa_K1399007
     stem_loop       859..902
                     /label=stem_loop
     misc_feature    848..927
                     /label=BBa_B0010
     terminator      848..927
                     /label=BBa_B0010
     stem_loop       943..962
                     /label=T7 TE
     misc_feature    969..969
                     /label=stop
     polyA_site      963..976
                     /label=polya
     misc_feature    936..976
                     /label=BBa_B0012
     terminator      936..976
                     /label=BBa_B0012
     terminator      848..976
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      781 atgaactata caaagctgca aacgacgaaa actacaacta cgctgacgct tcttaataat
      841 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      901 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
      961 cctttctgcg tttata
//