BBa_K1400001 1 BBa_K1400001 PTRE(2)GX Dual input promoter. Activation at tetO binding sites, repression at gal4 sites. 2014-10-05T11:00:00Z 2015-05-08T01:10:16Z This part was generated by modifying the native Gal promoter from Saccharomyces cerevisiae. This is a dual input promoter that can be activated and repressed by two different activating proteins (Tet and Gal4). Is based off the Gal promoter in Saccharomyces cerevisiae, which is a relatively strong promoter. It has two Tetr sites upstream of the TATA box. These can be bound by the tet repressor, or by activating variants using the Tetr binding domain like rtTA (tet binding domain, vp16 activating domain). 10bp downstream of the TATA box two Gal4 binding sites are added, which can be bound by Gal4 binding proteins or phusion activators like GEV (Gal4 binding domain, human estrogen receptor, vp16). The close proximity of these sites to the TATA box cause any binding protein (activating or repressing) to repress expression. The close proximity of these sites all seem to affect transcription rates of this promoter, as it has significantly less expression than the native Gal promoter. Thus this promoter can be used for complex control of expression false false _1778_ 0 18522 9 It's complicated false The major design consideration was the proximity of the repressing sites to the TATA box. 10bp seemed to cause strong repression while still not interfering too much with transcription when no proteins are bound. false Dylan Siriwardena annotation2427458 1 GAL4 binding site range2427458 1 312 328 annotation2427460 1 GAL4 binding site range2427460 1 330 346 annotation2427451 1 tetO range2427451 1 66 84 annotation2427456 1 TATA box range2427456 1 295 301 annotation2427449 1 tetO range2427449 1 1 18 BBa_K1400001_sequence 1 ccctatcagtgatagagatgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgccttccctatcagtgatagagaaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggaaccccgacaataccgggaccttcaaattaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttactattgttatttagattcttaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcgggcgacagccctccgacggaagactctcctccgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggagaaaaaac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z