BBa_K1400001
1
BBa_K1400001
PTRE(2)GX Dual input promoter. Activation at tetO binding sites, repression at gal4 sites.
2014-10-05T11:00:00Z
2015-05-08T01:10:16Z
This part was generated by modifying the native Gal promoter from Saccharomyces cerevisiae.
This is a dual input promoter that can be activated and repressed by two different activating proteins (Tet and Gal4). Is based off the Gal promoter in Saccharomyces cerevisiae, which is a relatively strong promoter. It has two Tetr sites upstream of the TATA box. These can be bound by the tet repressor, or by activating variants using the Tetr binding domain like rtTA (tet binding domain, vp16 activating domain). 10bp downstream of the TATA box two Gal4 binding sites are added, which can be bound by Gal4 binding proteins or phusion activators like GEV (Gal4 binding domain, human estrogen receptor, vp16). The close proximity of these sites to the TATA box cause any binding protein (activating or repressing) to repress expression. The close proximity of these sites all seem to affect transcription rates of this promoter, as it has significantly less expression than the native Gal promoter. Thus this promoter can be used for complex control of expression
false
false
_1778_
0
18522
9
It's complicated
false
The major design consideration was the proximity of the repressing sites to the TATA box. 10bp seemed to cause strong repression while still not interfering too much with transcription when no proteins are bound.
false
Dylan Siriwardena
annotation2427458
1
GAL4 binding site
range2427458
1
312
328
annotation2427456
1
TATA box
range2427456
1
295
301
annotation2427460
1
GAL4 binding site
range2427460
1
330
346
annotation2427451
1
tetO
range2427451
1
66
84
annotation2427449
1
tetO
range2427449
1
1
18
BBa_K1400001_sequence
1
ccctatcagtgatagagatgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgccttccctatcagtgatagagaaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggaaccccgacaataccgggaccttcaaattaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttactattgttatttagattcttaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcgggcgacagccctccgacggaagactctcctccgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggagaaaaaac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z