BBa_K1400004 1 BBa_K1400004 pGALtx Dual input promoter. Activation at gal4 binding sites, repression at tetO sites. 2014-10-05T11:00:00Z 2015-05-08T01:10:16Z This part was generated by modifying the native Gal promoter from Saccharomyces cerevisiae. This is a dual input promoter that can be activated and repressed by two different activating proteins (Tet and Gal4). Is based off the Gal promoter in Saccharomyces cerevisiae, which is a relatively strong promoter. It has four gal4 sites upstream of the TATA box, which can be bound by Gal4 binding proteins or phusion activators like GEV (Gal4 binding domain, human estrogen receptor, vp16). 10bp downstream of the TATA box two tetr binding sites are added. These can be bound by the tet repressor, or by activating variants using the Tetr binding domain like rtTA (tet binding domain, vp16 activating domain).The close proximity of these sites to the TATA box cause any binding protein (activating or repressing) to repress expression. The close proximity of these sites all seem to affect transcription rates of this promoter, as it has significantly less expression than the native Gal promoter. Thus this promoter can be used for complex control of expression false false _1778_ 0 18522 9 It's complicated false The major design consideration was the proximity of the repressing sites to the TATA box. 10bp seemed to cause strong repression while still not interfering too much with transcription when no proteins are bound. false Dylan Siriwardena annotation2427562 1 tetO range2427562 1 372 390 annotation2427561 1 tetO range2427561 1 351 369 annotation2427521 1 GAL4 binding site range2427521 1 108 124 annotation2427518 1 GAL4 binding site range2427518 1 7 23 annotation2427522 1 TATA box range2427522 1 335 340 annotation2427519 1 GAL4 binding site range2427519 1 26 42 annotation2427520 1 GAL4 binding site range2427520 1 44 60 BBa_K1400004_sequence 1 gaagtacggattagaagccgccgagcgggcgacagccctccgacggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggaaccccgacaataccgggaccttcaaattaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttactattgttatttagattcttaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaactgtccctatcagtgatagagatctccctatcagtgatagagatattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggagaaaaaac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z