LOCUS       BBa_K1401010            1141 bp    DNA     linear       26-Nov-2024
DEFINITION  Transcriptional Unit with pBad-pTet tandem promoter
ACCESSION   BBa_K1401010
VERSION     BBa_K1401010.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    139..157
                     /label=TetR 1
     misc_feature    139..192
                     /label=BBa_R0040
     promoter        158..163
                     /label=-35
     misc_feature    164..182
                     /label=TetR 2
     promoter        181..186
                     /label=-10
     promoter        139..192
                     /label=BBa_R0040
     RBS             205..288
                     /label=BCD2
     misc_feature    201..204
                     /label=MoClo Fusion Site B
     misc_feature    289..292
                     /label=MoClo Fusion Site C
     RBS             201..292
                     /label=BBa_K1114107
     CDS             299..973
                     /label=mrfp1
     misc_feature    980..1004
                     /label=Help:Barcodes
     CDS             299..1004
                     /label=BBa_E1010
     stem_loop       1024..1067
                     /label=stem_loop
     misc_feature    1013..1092
                     /label=BBa_B0010
     terminator      1013..1092
                     /label=BBa_B0010
     stem_loop       1108..1127
                     /label=T7 TE
     misc_feature    1134..1134
                     /label=stop
     polyA_site      1128..1141
                     /label=polya
     misc_feature    1101..1141
                     /label=BBa_B0012
     terminator      1101..1141
                     /label=BBa_B0012
     terminator      1013..1141
                     /label=BBa_B0015
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagtc cctatcagtg atagagattg acatccctat cagtgataga
      181 gatactgagc actactagag tactgggccc aagttcactt aaaaaggaga tcaacaatga
      241 aagcaatttt cgtactgaaa catcttaatc atgctaagga ggttttctaa tgtactagat
      301 ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc
      361 cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac
      421 ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct
      481 gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga
      541 ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga
      601 cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa
      661 agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat
      721 gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat
      781 caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta
      841 catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat
      901 cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca
      961 ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat
     1021 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1081 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1141 a
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