##gff-version 3
##sequence-region BBa_K1401010 1 1141
BBa_K1401010	.	promoter	139	192	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1401010	.	promoter	181	186	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1401010	.	promoter	158	163	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1401010	.	engineered_region	139	192	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1401010	.	non_covalent_binding_site	164	182	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1401010	.	non_covalent_binding_site	139	157	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1401010	.	CDS	299	1004	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K1401010	.	CDS	299	973	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1401010	.	engineered_tag	980	1004	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1401010	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_K1401010	.	terminator	1013	1141	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1401010	.	terminator	1101	1141	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1401010	.	engineered_region	1101	1141	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1401010	.	stem_loop	1108	1127	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1401010	.	stop_codon	1134	1134	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1401010	.	polyA_site	1128	1141	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1401010	.	terminator	1013	1092	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1401010	.	engineered_region	1013	1092	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1401010	.	stem_loop	1024	1067	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1401010	.	ribosome_entry_site	201	292	.	+	0	ID=BBa_K1114107;Name=BBa_K1114107
BBa_K1401010	.	sequence_feature	201	204	.	+	0	ID=annotation2361727;Name=MoClo Fusion Site B;Parent=BBa_K1114107
BBa_K1401010	.	ribosome_entry_site	205	288	.	+	0	ID=annotation2361724;Name=BCD2;Parent=BBa_K1114107
BBa_K1401010	.	sequence_feature	289	292	.	+	0	ID=annotation2361728;Name=MoClo Fusion Site C;Parent=BBa_K1114107
>BBa_K1401010
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagtccctatcagtgatagagat
tgacatccctatcagtgatagagatactgagcactactagagtactgggcccaagttcacttaaaaaggagatcaacaa
tgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgtactagatggcttcctccgaagac
gttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaag
gtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttggga
catcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactg
tccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcct
ccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaa
aaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgt
ctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgg
gtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgc
tgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttata