BBa_K1114107
1
BBa_K1114107
Bicistronic Design RBS 2, MoClo Format with BC fusion sites
2013-09-06T11:00:00Z
2015-05-08T01:09:11Z
This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by <a href= ???http://www.nature.com/nmeth/journal/v10/n4/full/nmeth.2404.html????>Mutalik, et al.</a>
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_K783055???>BBa_K783055</a>.
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false
false
_1425_
0
8248
9
In stock
false
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites.
This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_K783055???>BBa_K783055</a>.
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false
Traci Haddock
annotation2361728
1
MoClo Fusion Site C
range2361728
1
89
92
annotation2361724
1
BCD2
range2361724
1
5
88
annotation2361727
1
MoClo Fusion Site B
range2361727
1
1
4
BBa_K1401606
1
BBa_K1401606
pBad- BCD2 - RFP
2014-10-10T11:00:00Z
2015-05-08T01:10:16Z
E. Coli
The parts contain the BioBricks - I13453_FB, BCD2_BC, E1010_CD and B0015_DG. All of these are MoClo variants i.e. they have MoClo flanking regions attached to each basic part.
false
false
_1779_
0
23227
9
Not in stock
false
All basic BioBricks used were transformed into MoClo variants by adding the following fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. These were then put together using the digestion-ligation MoClo reaction.
false
Yash Agarwal
component2417170
1
BBa_K1114210
component2417169
1
BBa_K1114107
component2417174
1
BBa_K1114301
component2417165
1
BBa_K1114024
annotation2417170
1
BBa_K1114210
range2417170
1
247
935
annotation2417169
1
BBa_K1114107
range2417169
1
147
238
annotation2417174
1
BBa_K1114301
range2417174
1
944
1080
annotation2417165
1
BBa_K1114024
range2417165
1
1
138
BBa_K1114210
1
BBa_K1114210
E1010_CD
2013-09-06T11:00:00Z
2015-05-08T01:09:12Z
igem kit
RFP Reporter E1010 in Moclo form
false
false
_1425_
0
13936
9
In stock
false
n'a
false
Shawn Jin
BBa_K1114024
1
BBa_K1114024
MoClo version of I13453 with FB fusion sites
2013-09-11T11:00:00Z
2015-05-08T01:09:11Z
iGEM Distribution Kit
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This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_I13453 with FB fusion sites. See the <a href="http://parts.igem.org/Part:BBa_I13453">BBa_I13453</a> page for full information on the part.
This is a MoClo part fusion sites F on the 5' side and site B on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_K1114412???></a>.
</html>
false
false
_1425_
0
8248
9
In stock
false
<html>
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_I13453 with FB fusion sites. See the <a href="http://parts.igem.org/Part:BBa_I13453">BBa_I13453</a> page for full information on the part.
This is a MoClo part fusion sites F on the 5' side and site B on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_K1114412???></a>.
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false
Traci Haddock
BBa_K1114301
1
BBa_K1114301
This is MoClo formatted part of BBa_B0015
2013-09-06T11:00:00Z
2015-05-08T01:09:12Z
iGEM kit
This is a Level 0 MoClo part with flanking sites D on the 5' side and site G on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_ K1114409 ???>BBa_</a>.
false
false
_1425_
0
17246
9
In stock
false
This is a Level 0 MoClo part with flanking sites D on the 5' side and site G on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_ K1114409 ???>BBa_</a>.
false
Devina Desai
annotation2362001
1
MoClo Fusion Site G
range2362001
1
134
137
annotation2362002
1
BBa_B0015
range2362002
1
5
133
annotation2362000
1
MoClo Fusion Site D
range2362000
1
1
4
BBa_K1114210_sequence
1
aatgatggcttcctccgaggatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataaaggt
BBa_K1114107_sequence
1
tactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatg
BBa_K1114301_sequence
1
aggtccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatgcc
BBa_K1114024_sequence
1
cgctacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctact
BBa_K1401606_sequence
1
cgctacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctacttactagagtactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgtactagagaatgatggcttcctccgaggatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataaaggttactagagaggtccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatgcc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z