LOCUS BBa_K1401607 1110 bp DNA linear 22-Nov-2024 DEFINITION pLac - BCD2 - tetR ACCESSION BBa_K1401607 VERSION BBa_K1401607.1 FEATURES Location/Qualifiers misc_feature 1..200 /label=BBa_R0010 CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_K783046 RBS 213..296 /label=BCD2 misc_feature 209..212 /label=MoClo Fusion Site B misc_feature 297..300 /label=MoClo Fusion Site C RBS 209..300 /label=BBa_K1114107 CDS 309..973 /label=BBa_K1114202 stem_loop 993..1036 /label=stem_loop misc_feature 982..1061 /label=BBa_B0010 terminator 982..1061 /label=BBa_B0010 stem_loop 1077..1096 /label=T7 TE misc_feature 1103..1103 /label=stop polyA_site 1097..1110 /label=polya misc_feature 1070..1110 /label=BBa_B0012 terminator 1070..1110 /label=BBa_B0012 terminator 982..1110 /label=BBa_K783020 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagta ctgggcccaa gttcacttaa aaaggagatc 241 aacaatgaaa gcaattttcg tactgaaaca tcttaatcat gctaaggagg ttttctaatg 301 tactagagaa tgtccagatt agataaaagt aaagtgatta acagcgcatt agagctgctt 361 aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag 421 cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt 481 gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat 541 tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca 601 aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta 661 gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg 721 gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa 781 agggaaacac ctactactga tagtatgccg ccattattac gacaagctat cgaattattt 841 gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta 901 gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta cgctttagta 961 gcttaataaa ggttactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 1021 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 1081 caccttcggg tgggcctttc tgcgtttata //