LOCUS       BBa_K1401607            1110 bp    DNA     linear       14-Nov-2024
DEFINITION  pLac - BCD2 - tetR
ACCESSION   BBa_K1401607
VERSION     BBa_K1401607.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=BBa_R0010
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_K783046
     RBS             213..296
                     /label=BCD2
     misc_feature    209..212
                     /label=MoClo Fusion Site B
     misc_feature    297..300
                     /label=MoClo Fusion Site C
     RBS             209..300
                     /label=BBa_K1114107
     CDS             309..973
                     /label=BBa_K1114202
     stem_loop       993..1036
                     /label=stem_loop
     misc_feature    982..1061
                     /label=BBa_B0010
     terminator      982..1061
                     /label=BBa_B0010
     stem_loop       1077..1096
                     /label=T7 TE
     misc_feature    1103..1103
                     /label=stop
     polyA_site      1097..1110
                     /label=polya
     misc_feature    1070..1110
                     /label=BBa_B0012
     terminator      1070..1110
                     /label=BBa_B0012
     terminator      982..1110
                     /label=BBa_K783020
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagta ctgggcccaa gttcacttaa aaaggagatc
      241 aacaatgaaa gcaattttcg tactgaaaca tcttaatcat gctaaggagg ttttctaatg
      301 tactagagaa tgtccagatt agataaaagt aaagtgatta acagcgcatt agagctgctt
      361 aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag
      421 cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt
      481 gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat
      541 tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca
      601 aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta
      661 gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg
      721 gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa
      781 agggaaacac ctactactga tagtatgccg ccattattac gacaagctat cgaattattt
      841 gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta
      901 gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta cgctttagta
      961 gcttaataaa ggttactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1021 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1081 caccttcggg tgggcctttc tgcgtttata
//